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Pertoft, H. (2000) Fractionation of Cells and Subcellular Particles with Percoll. Journal of Biochemical and Biophysical Methods, 44, 1-30. http://dx.doi.org/10.1016/S0165-022X(00)00066-X

has been cited by the following article:

  • TITLE: Percoll Gradient Optimization for Blood CD133+ Stem Cell Recovery

    AUTHORS: Rosa María López-Franco, Jorge E. Moreno-Cuevas, María Teresa González-Garza

    KEYWORDS: Stem Cells Recovery, CD133+, Percoll, Density Gradients

    JOURNAL NAME: Stem Cell Discovery, Vol.4 No.3, July 3, 2014

    ABSTRACT: Circulating CD133+ stem cells from the peripheral blood have been shown to be able to differentiate into numerous cell lineages. However, adults have only a small number of these circulating stem cells. The aim of the present study was to assess a new isolation and enrichment technique for CD133+ stem cells from peripheral blood with the use of Percoll density gradients. Our results demonstrated the presence of two large mononuclear bands when whole blood was centrifuged with 48% and 50% Percoll concentrations. Flow cytometric analysis (FACS) revealed a major CD133+ enrichment at the 48% Percoll concentration in one of the two bands. Further culture of these cells resulted in the formation of multiple colony-forming units. Our results suggest an advantage from using a simple Percoll gradient for successful CD133+ cell recovery, which could aid in differentiation and transplantation protocols.