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Kuci, S., Wessels, J.T., Buhring, H.J., Schilbach, K., Schumm, M., Seitz, G., Loffler, J., Bader, P., Schlegel, P.G., Niethammer, D. and Handgretinger, R. (2003) Identification of a Novel Class of Human Adherent CD34-Stem Cells That Give Rise to SCID-Repopulating Cells. Blood, 101, 869-876.

has been cited by the following article:

  • TITLE: Percoll Gradient Optimization for Blood CD133+ Stem Cell Recovery

    AUTHORS: Rosa María López-Franco, Jorge E. Moreno-Cuevas, María Teresa González-Garza

    KEYWORDS: Stem Cells Recovery, CD133+, Percoll, Density Gradients

    JOURNAL NAME: Stem Cell Discovery, Vol.4 No.3, July 3, 2014

    ABSTRACT: Circulating CD133+ stem cells from the peripheral blood have been shown to be able to differentiate into numerous cell lineages. However, adults have only a small number of these circulating stem cells. The aim of the present study was to assess a new isolation and enrichment technique for CD133+ stem cells from peripheral blood with the use of Percoll density gradients. Our results demonstrated the presence of two large mononuclear bands when whole blood was centrifuged with 48% and 50% Percoll concentrations. Flow cytometric analysis (FACS) revealed a major CD133+ enrichment at the 48% Percoll concentration in one of the two bands. Further culture of these cells resulted in the formation of multiple colony-forming units. Our results suggest an advantage from using a simple Percoll gradient for successful CD133+ cell recovery, which could aid in differentiation and transplantation protocols.