TITLE:
Antibacterial Activity of Polyethylenimine/Carrageenan Multilayer against Pathogenic Bacteria
AUTHORS:
Annabelle V. Briones, Toshinori Sato, Ursela G. Bigol
KEYWORDS:
Carrageenan, Polyethylenimine, Bacterial Growth Inhibition, Anti-Bacterial
JOURNAL NAME:
Advances in Chemical Engineering and Science,
Vol.4 No.2,
April
18,
2014
ABSTRACT:
The multilayer
of polyethylenimine (PEI) and carrageenan (k, i, l) formed by layer-by-layer assembly
was investigated for its antibacterial activity against Enterobacter cloaceae, Staphylococcus aureus and Enterococcus faecalis 29505 for potential use as coating
on biomaterial surface. All the multilayers exhibited growth inhibition. PEI/Iota carrageenan multilayer was effective
in inhibiting the growth of the E. cloaceae, S. aureus and E. faecalis while PEI/Lambda carrageenan was effective
in inhibiting the growth of E. cloaceae. Results of the paper strip test
for combined action of carrageenan and PEI showed synergism with regards to bacterial
growth inhibition. The multilayers had also contact-killing effect with the test
organisms. The multilayer was also characterized by atomic force microscopy (AFM),
X-ray photoelectron spectroscopy (XPS) and biomolecular interaction analysis.