TITLE:
Development of a Genetic Transformation Method for Seabuckthorn (Hippophae rhamnoides L.)
AUTHORS:
Sridevy Sriskandarajah, David Clapham, Per-Olof Lundquist
KEYWORDS:
Hippophae rhamnoides; Particle Bombardment; Transformation
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.5,
March
7,
2014
ABSTRACT:
Seabuckthorn (Hippophae
rhamnoides L.) is a dioecious plant with berries containing high amounts of
several bioactive compounds with nutritional and medicinal traits. It is also planted to control soil erosion. A genetic transformation
procedure will facilitate studies of the control of plant development and
interactions with symbionts and pathogens, and will provide a tool for plant
breeding. Here, we present a particle bombardment
method for transforming seabuckthorn. The early stages of induced adventitious
shoots from roots were chosen as a novel target tissue for the transformation
procedure. The root system was bombarded with gold particles coated with
plasmid pRT99gus containing genes for plant kanamycin resistance and for β-glucuronidase expression,
and shoots were regenerated under kanamycin selection. PCR analysis of the
regenerated transformed lines confirmed the presence of a 603 bp gus (uidA)
gene fragment and a 1.5 kb fragment from the 35S promoter in three shoots from
independent transformation events.