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Mukhopadhya, I., Thomson, J.M., Hansen, R., Berry, S.H., El-Omar, E.M. and Hold, G.L. (2011) Detection of Campylobacter concisus and Other Campylobacter Species in Colonic Biopsies from Adults with Ulcerative Colitis. PLoS One, 6, e21490.

has been cited by the following article:

  • TITLE: Development and Evaluation of a Multiplex PCR for the Detection of Campylobacter concisus and Other Campylobacter spp. from Gastroenteritis Cases

    AUTHORS: Mohsina Huq, Gena Gonis, Taghrid Istivan

    KEYWORDS: Campylobacter concisus; Campylobacter spp.; Gastroenteritis; Faecal Spiking; Multiplex PCR

    JOURNAL NAME: Open Journal of Medical Microbiology, Vol.4 No.1, February 19, 2014

    ABSTRACT: We developed and evaluated a multiplex PCR (m-PCR) for application in routine diagnostic laboratories to detect Campylobacter spp. in stool samples including C. concisus, C. jejuni, and C. coli. When this m-PCR was applied on spiked faecal samples, C. concisus, C. jejuni, and C. coli were specifically identified at 105 cells/gm of faeces. To compare the sensitivity of the m-PCR with conventional culture techniques, the same spiked stool samples were cultured on an antibiotic free Columbia blood agar using the filtration technique. The detection limit of conventional culture method was 105 cells/gm of stool for C. concisus and 106 cells/gm of stool for C. jejuni and C. coli. The m-PCR was applied to test 127 faecal samples from children with gastroenteritis and the results were compared with the conventional bacterial cultures data. By this m-PCR technique, C. jejuni was detected in 7 samples, C. coli in 2 samples, and C. concisus in 7 samples. However, the conventional culture results for these samples were 6 for C. jejuni, 2 for C. coli and only one sample was positive for C. concisus. In total, 19 samples were positive for Campylobacter spp. by m-PCR while only 9 samples were positive for Campylobacter spp. by culture. In conclusion, m-PCR is more sensitive than the culture technique to detect C. concisus and other fastidious campylobacters in faeces.