TITLE:
Vasodilator response to Psidium guajave leaves of mesenteric bed vessels from diabetic and non-diabetic rats
AUTHORS:
Habeib Zaheri, Golam Reza Farshidfar, Nepton Soltani, Fatemah Kharazmi, Mohammad Kamalinajad
KEYWORDS:
Psidium guajave Linn Extract; Diabetes Mellitus; Mesenteric Bed; Vasorelaxation; Nitric Oxide
JOURNAL NAME:
Journal of Diabetes Mellitus,
Vol.4 No.1,
January
8,
2014
ABSTRACT:
Mortality from
cardiovascular abnormalities is almost three times more prevalent in the diabetic
population than that in the general population. Psidium guajave has been
used traditionally for a long time as a medicinal herb to cure diabetes.
Endothelial dependent vasorelaxation effect of Psidium guajave extract
on normal aortic rings was observed. This study was designed to investigate the endothelium and nitric oxide
roles inPGE-induced vasorelaxation
in diabetic rat vessel. Diabetes was induced by a single intraperitoneal injection of streptozotocin. Eight
weeks later, superior mesenteric arteries of non-diabetic and diabetic
groups were isolated and perfused according to the McGregor method. Prepared
vascular beds were constricted with phenylephrine
to induce 70% - 75% of maximal constriction. Psidium guajave extract at concentrations of 0.1 to 4 mg/100ml added to
the medium and perfusion pressure was recorded. Baseline perfusion pressure of
diabetic group was significantly higher than non-diabetic rats in both intact
and denuded endothelium. Psidium guajave extract caused a significantly
dose depended decrease in perfusion pressure in non-diabetic and diabetic
groups in both intact and denuded endothelium. In the presence of N (ω)-nitro-L-arginine methyl ester, Psidium
guajave ex-tractinduced relaxation in intact mesenteric beds of non-diabetic
and diabetic animals was not suppressed. From the results of this study, it may
be concluded that vasorelaxatory effect of Psidium guajave extract is
not mediated by endothelium and nitric oxide in both diabetic and non-diabetic
vessel and supports the use of the plant’s leaves as a natural, adjunct
phytomedicine in the management of diabetes vessel complications.