Determining the Prevalence of Bacterial Vaginosis & Vulvovaginal Candidiasis among Married and Unmarried Women & Evaluating the Association Socio-Demographic Risk Factors & Symptoms-Related Variables in Women Attending Gynecology Clinic in Hargeisa Group Hospital, Hargeisa City, Somaliland

Background: Vaginitis refers to any inflammation or infection of the vagina. This is a common gynecological problem found in women of all ages, with one-third of women having at least one form of vaginitis at some time during their lives. The vagina is the muscular passageway between the uterus and the external genital area. When the walls of the vagina become in-flamed, because some irritant has disturbed the balance of the vaginal area, vaginitis can occur. The most common types of vaginitis are: Candida or “yeast” infection, Bacterial vaginosis, Trichomoniasis vaginitis. Objectives of Study: Therefore, the present study was carried out to determine the prevalence of bacterial vaginosis (BV), vulvovaginal candidiasis (VVC) among married and unmarried women and evaluate the association so-cio-demographic risk factors and symptoms-related variables in women attending gynecology clinic in Hargeisa group hospital. Methodology: A total of 150 married and unmarried women were investigated & diagnosed by a researcher in Hargeisa Group Hospital, Hargeisa City, Somaliland for determining of VVI prevalence in the present study. Vaginal swabs from these variation in the prevalence rates of bacterial vaginosis & vagina candidasis. Conclusion: VVC was the most prevalent VVI followed by BV in Hargeisa City. C. albicans was the most prevalent species in VVC while among BV species, Gardnerella vaginalis was found to occur at highest frequency. However, further studies are needed to assess specific diagnosis and role of clinical risk factors. Urgent action is required to improve vagina infection control measures to reduce the prevalence and make new policies for treatment of vagina infection in HGH.


Introduction
Vaginitis refers to any inflammation or infection of the vagina. This is a common gynecological problem found in women of all ages, with one-third of women having at least one form of vaginitis at some time during their lives [1].
The vagina is the muscular passageway between the uterus and the external ge-  [3]. Candida genital infection is the leading cause of fungal vulvovaginitis. Pregnancy, broad-spectrum antibiotic use, diabetes mellitus, and immunodeficiency have been described as important risk factors for Candida genital infection; however, asymptomatic microorganism colonization can occur in 25% to 50% of the cases [4] [5] [6].
Bacterial vaginosis is described as a shift in the balance of the vaginal microflora characterized by an increase in the vaginal pH, a reduction in lactobacilli, predominantly hydrogen peroxide producing species, and an increase in facultative and anaerobic bacteria in number and/or type [7]. Although the prevalence of bacterial vaginosis differs widely from country to country within the same region and even within similar population groups, it has been estimated to be in the range of 8% to 75% [2]. Bacterial vaginosis can occur in any age group, but globally it is more prevalent in females of reproductive age [8]. Furthermore, bacterial vaginosis & vagina candidiasis have been associated with increased susceptibility to HIV-AIDS and other STD [9].
Symptoms and signs of vaginitis may include the change in color, odor, or amount of vaginal secretions, pain during intercourse, painful urination, and light vaginal bleeding or spotting. The characteristics of vaginal discharge may indicate the type of vaginitis [10]. Previous data from epidemiological surveys show that within countries and between countries in the same region, the prevalence and incidence of genital tract infections may vary widely even in similar population groups [3]. In developing countries, patients with genital tract infections often seek care through formal healthcare services and in the informal sector [2] [11]. Diverse studies performed, with the objective of establishing the frequency of the most common infectious agents for vaginitis, have shown varying results. The prevalence found for BV has varied, ranging between 8% and 75%; VVC has presented 2.2% -30% and trichomoniasis between 0% and 34% [12]. VVC is an extremely common infection in women of childbearing age of all strata of society. Since it has now been excluded from the ranks of sexually transmitted diseases and is also not a notifiable disease, not much information regarding its incidence and epidemiology is available [9]. VVC is the second most common cause of vaginitis in the United States and the most common cause in Europe [6] Infection of the female genital tract can result in vaginitis, cervicitis, and urethritis, and trichomoniasis has been associated with adverse pregnancy outcomes [13]. Each slide was then graded as per the standardized quantitative morphological classification method developed by Nugent et al. [14].

Results Analysis
In the current study a total of one hundred fifty (150) high vaginal swabs samples were investigated from women the results in Table 1  The results in present study indicated in the ( Table 3) that BV to be the most common cause are Gardnerella vaginalis 22 (19.8%) followed by Lactobacillus spp. 11 (9.9%) and Streptococcus agalactiae 7 (6.3%). While the distribution of VVC species the results indicated that Candida albicans species most common with 47 (42.3%) followed by C. tropicalis with 9 (8.1%) and C. glabrata 7 (6.3%).
All species were further confirmed by germ tube test and morphological characteristics such as Saboraud Dextrose Agar (SDA) culture, rice meal agar test, CHROM agar medium and sugar fermentation test as showed also in ( Figures   1-3 and Table 4).      Figure 4 shows the socio-demographic characteristics of patients. The age range of the married & unmarried women was between 18 years and 60 years, with a mean age of 30.55 ± 6.171. The prevalence of BV decreased with VVC age from 18 years up to the age of 20 while, the prevalence of BV & VVC it was increasing with age from 21 years up to 35 years. The results also showed more married women aged 21 -35 years had BV & VVC than those in other age groups. On the other hand, the results showed the residency were increasing with BV in the urban area it was 32 (74.4%) & VVC it was with rural 47 (69.1%). The study also showed statistical significant difference (P < 0.5) between patients which belonged to the low monthly income class, housewife's occupation and illiterates educational level. The results in the present study as showed in (  The results in (

Discussion
Diagnosis & analysis of vaginal swabs had indicated the presence of BV and VVC while no case of trichomoniasis was detected. VVC was found to be the  1). This finding is in consonance with various earlier studies which have indicated VVC to be the most prevalent cause of BV. However, percentage prevalence of BV in the present study was found to be comparatively very similar with other reported in earlier studies whereas bacterial vaginosis was found in 38(38%) of study participants [15]. BV percentage in our study also compared with that (1.4%) reported by [4] among women attending antenatal and gynaecological clinic in Accra. However, it was comparable to a prevalence of (28.0%) reported among non-pregnant women in the coastal area of Ghana [2]. Another study reported a prevalence of 47.0% among women with incomplete abortion in Accra [16]. This high rate was probably due to the complication of the abortion which could alter the normal flora of the vagina. Other reported an aggregate rate of 54% on women presenting with vaginal discharge in five West African states including Ghana [17]. In other West African states, the prevalence of BV of 64.3, 17.3 and 6.4% were reported in Southwestern-Nigeria, Northeastern-Nigeria and Burkina Faso respectively in pregnant women [18]. Higher prevalence rates of BV than those in the present study were also reported by [19] in Egypt (33%). The variation in the findings might be due to population size, methods of analysis, geographic distribution, and socioeconomic and behavioral differences in the studied population. On the other hand, prevalence of VVC in our study 68 (45%) found to be comparatively highly similar 39 (48%) with other studies from India and some other countries [20] [21]. The VVC also reported rate among women compares well with (34.2%) in Accra, (36.0%) in Southwestern Nigeria, (37.4%) in Turkey [4] [22] [23]. No case of trichomoniasis was found in our study groups. This finding is in agreement with some earlier reports which have documented very low prevalence (2%) of trichomoniasis [17] [20]. However, the prevalence of trichomoniasis appears to be relatively lower than the other vaginal infections and seems to have declined when compared with earlier reports [24]. These differences in the percentage of prevalence of among types of VVI can be attributed to environmental, behavioral, socioeconomic status and stressor differences in the geographical variation [25]. Recall, this disparity in the prevalence also might be due lack of proper sanitary conditions in many rural communities such as Hargiesa compared to other developing countries. Risk factors for the infection and local population also play dynamics accounts for the huge disparities in the prevalence rate across countries. In addition to, geographical distribution, vaginal hygiene practices and systematic difference in the various populations sampled could account for the variation in the prevalence values. All of the Candidiasis morphotypes were indicated by creamy white smooth colonies on SDA culture (Figure 1) For identification of Candida species in the positive patients for VVC including were further examined by positive germ tube test, green stain in CHROMagar Candida medium (Figure 2), carbohydrates fermentation test ( Figure 3) and rice meal agar test (Table 4). C. albicans found in our study as showed (Table 3) to be the single most prevalent species with 47 (42.3%) followed by C. tropicalis 9 (8.1%) in VVC. This finding Open Journal of Medical Microbiology of the present study was similar to the previous studies Int. J. Curr. Microbiol. [20] conducted in Nigeria, USA, Kenya, India, UAE, Iran [17]. However, this is in contrast to a single study reported in India where C. tropicalis was found to be more prevalent than C. albicans [26]. The high Candida spp colonization/infection of current study patients' vagina could have been due to their pregnancy & marital status which plays a major role in colonization and infection. Study demonstrates frequent colonization of the VVC of married women with Candida spp compared to unmarried women (Table 3). This is as a result of the high concentration of estrogen during married which provides a favorable environment for the growth of Candida spp [27]. VVC could also be an indication of an underlying infection such as diabetes mellitus. On the other hand, Gardnerella vaginalis was 22 (19.8%) as hazardous in BV pathogenesis and symptomatology followed by Lactobacillus spp., recorded also high 11 (9.9%) among BV positive married and unmarried women, was found to be one of most prevalent natural micro biota of the lower genital tract in women. While, Streptococcus agalactiae was 7 (6.3%). Elevation alters of pH in vagina women above age of 45 years has been identified as a cause of a decline in the level of estrogen, which in turn creates an optimal condition for the growth of bacteria other than lactobacilli & depletion of lactic acid also producing lactobacillus. On the other hand, the presence of lactobacilli together with other opportunistic pathogens may be due to several factors like effects of antibiotics, type of incubation (as some Lactobacillus spp. are unable to produce some defense factors under anaerobic incubation), and antagonism among lactobacilli species to maintain dominance [28]. According to our study the results found a high prevalence of vaginal infections (BV) among married women 32 (74.4%) while, vulvovaginal candidiasis VVC) was higher among unmarried women 47 (69.1%) ( Table 5). These findings among married women and unmarried women are in consonance with previous studies [4] [22]. The finding of the study also was contradicted by [19] in Egypt. However, several studies have documented the occurrence of BV in sexually inactive females or virgins [14] [29]. This provides evidence that sexual activity is not a prerequisite for BV. The change in lifestyle, improper perineal care, food habits, tight clothing, lack of attention towards menstrual hygiene, and sedentary factor might be the reasons for the acquisition of BV in unmarried women. The incidence of vagina infections has greatly increased more especially with the married women aged 21 -35 years among both BV & VVC patients than those were least for 18 -20 and 60 years' age groups 7 (16.3%) 15 (34.8%) respectively. The result also similar with [21] in Nigeria who found BV to be most prevalent among 26 -30 age group (35.8%).The highest prevalence in the age group 21 -35 years might be due to the age being the most reproductively active age group and high sexual exposure at this age. According to, the results showed the residency were increasing with BV in the urban area it was 32 (74.4%) & VVC it was increasing among rural patients 47 (69.1%). The study also showed statistical significant difference between patients which belonged to the low A. Al-Mamari Open Journal of Medical Microbiology monthly income class, housewife's, illiterates educational level and menstrual hygienic pads (Figure 4). However, sociodemographic characteristics, sexual activity, reproductive health information, and behavioral and genital hygiene have been identified as causes of variation in the prevalence rates of BV& VVC [30] [31]. In this investigation, the most common clinical sign and symptoms such as itching, odor, and amount of vaginal discharge were found to be statistically significant relationship (P value < 0.05) with BV & VVC ( Figure 5). This finding corresponds with previous study conducted by [21] in Nigeria and other studies done in different countries [22] [26] [32].

Conclusion
The overall prevalence of bacterial vaginosis and vulvovaginal candidiasis among married and unmarried women in this study indicated that VVC was more prevalent among married women while, BV was more prevalence among unmarried women. Therefore, further studies for investigating bacterial vaginosis and vul-

Conflicts of Interest
The author declares no conflicts of interest regarding the publication of this paper.