Inquiry of Water-Soluble Polysaccharide Extraction Conditions from Grapefruit Skin
Ziping Luo, Zilin Liu, Shiyu Tan, Qiliang Xu, Haiyan Yang, Lingli Yang
DOI: 10.4236/eng.2011.311135   PDF    HTML     5,728 Downloads   10,150 Views   Citations


Water-soluble polysaccharide was isolated from grapefruit with the method of water-dissolving and ethanol-precipitating. By single factor test and orthogonal experimental method, the extraction conditions of the water-soluble polysaccharide were investigated, which contained liquid-solid ratio, temperature, time and pH. The results showed that pH was the most important factor of polysaccharide extraction, followed by temperature. The optimized extraction technology conditions were obtained. The liquid-solid ratio was 1:40, temperature was 90?C, time was 2.0 h, and pH was 2. The highest extraction rate of crude polysaccharide was 8.30%.

Share and Cite:

Z. Luo, Z. Liu, S. Tan, Q. Xu, H. Yang and L. Yang, "Inquiry of Water-Soluble Polysaccharide Extraction Conditions from Grapefruit Skin," Engineering, Vol. 3 No. 11, 2011, pp. 1090-1094. doi: 10.4236/eng.2011.311135.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] H. L. Jin and Z. R. Xu, “Antiviral and Immune Research Progress of Polysaccharide,” Feed Expo, Vol. 1, 2002, pp. 28-30.
[2] C. G. Qin, K. X. Huang and H. B. Xu, “Isolation and Characterization of Novel Polysaccharides from the Mucus of the Loach, Misgurnus Anguillicaudatus,” Carbohydrate Polymer, Vol. 49, No. 3, 2002, pp. 367-371. doi:10.1016/S0144-8617(01)00335-6
[3] F. Yamaguchif, Y. Ota and C. Hatanaka, “Extraction and Purification of Pectic Polysaccharides from Soybean Okara and Enzymaticanalysis of Their Structures,” Carbohydrate Polymer, Vol. 30, No. 4, 1996, pp. 265-273. doi:10.1016/S0144-8617(96)00046-X
[4] D. Y. Jia, K. Yao and M. Tan, “The Physiological Activity Component in Shaddock Are Reviewed,” Food and Fermentation Industry, Vol. 27, No. 11, 2001, pp. 74-78.
[5] X. Li, “The Extraction and Test of Polysaccharide in Shaddock Ped,” Value Engineering, Vol. 29, No. 18, 2010, pp. 255-256.
[6] Y. J. Fan, Q. Zhang and B. Zhu, “Study on Extraction of Soluble Soybean Polysaccharides,” Food Science, Vol. 28, No. 9, 2007, pp. 295-298.
[7] Q. Dong, L. Y. Zheng and J. N. Fang, “Modified Phenol Sulfuric Acid Method for Determination of Content of Oligo and Polysaccharides,” Chinese Pharmaceutical, Vol. 31, No. 9, 2001, pp. 550-553.
[8] X. H. Yue, “Research of Edible Chitosan and Starch Composite Membrane,” Food Science, 2004, pp. 7-10.
[9] H. Q. Xu and Z. H. Wei, “Production Process of Water Insoluble Edible Film of Chitosan and Starch,” Food Research and Development, Vol. 22, 2001, pp. 20-22.

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.