DNA Sequence-Based Markers for Verification of Ramet-to-Ortet Relationship in Oil Palm (Elaeis guineensis Jacq.)


Since oil palm (Elaeis guineensis Jacq.) does not breed true, tissue culture-derived material is the resource of choice for achieving oil palm homogeneity in terms of growth and yield. Currently, no genomic tagging method is available with which one could verify the ortet (tissue donor oil palm) from which the clonal planting material (ramets) origi-nated, particularly in cases of unsatisfactory performance. Thus, Sime Darby Plantation used 10 genomic microsatellite markers to genotype 8 sets of 5 ramets +1 ortet and 2 single ortets. The average genetic distance (D) among oil palms from the same set of ramets and ortet was 0.0000 with the exception of sets containing off-type ramets (D = [0.0834 - 0.1505]). The dendrogram showed that the ramets and their ortet of origin formed a sub-cluster, confirming their simi-larity. The 10 microsatellite markers were valuable to Sime Darby as tools for verification of ramet-to-ortet relation-ships and for the identification of off-types. Furthermore, the set of 10 markers revealed a high expected heterozygosity (He = 0.634) that is a high expected heterosis effect on which yield depends.

Share and Cite:

C. Bakoumé, M. Aziah, T. Praveena, C. Teh, Y. Suzaini, M. Hamidah, M. Jangi, M. Basiran, H. Khairudin and K. Harikrishna, "DNA Sequence-Based Markers for Verification of Ramet-to-Ortet Relationship in Oil Palm (Elaeis guineensis Jacq.)," American Journal of Plant Sciences, Vol. 2 No. 4, 2011, pp. 539-548. doi: 10.4236/ajps.2011.24064.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] N. Rajanaidu and A. Kushairi, “Strategies for the Devel- opment of Future Oil Palm Planting Materials,” Paper Presented at ISOPB Seminar on the Progress of Oil Palm Breeding and Selection, Medan, 6-9 October 2003.
[2] S. Mayes, F. Hafeez, Z. Price, D. MacDonald, N. Billotte and J. Roberts, “Molecular Research in Oil Palm, the Key Oil Crop for the Future,” In: P. H. Moore and R. Ming, Eds., Genomics in Tropical Crop Plants, Nottingham University Press, Nottingham, 2008, pp. 371-404. doi:10.1007/978-0-387-71219-2_15
[3] G. Van Der Linden, S. A. Sharifah, G. Angenent, S. C. Cheah and R. Smulders, “Molecular Characterization of Flower Development in Oil Palm in Relation to the Mantling Abnormality,” Proceedings MPOB 2001 International Palm Oil Congress―Agriculture, Kuala Lumpur, 2005, pp. 531-549.
[4] V. Le Guen, G. Samaritaan, Z. Othman, C. W. Chin, K. E. Konan and T. Durand-Gasselin, “Oil Production in Young Oil Palm Clones,” Oleagineux, Vol. 46, 1991, pp. 347-359.
[5] Y. Duval, P. Amblard, A. Rival, E. Konan, S. Gogor and T. Durand-Gasselin, “Progress in Oil Palm Tissue Culture and Clonal Performance in Indonesia and C?te d’Ivoire,” Proceedings ISP Conference Plantation Management for 21st Century, Kuala Lumpur, 1997, pp. 291-307.
[6] A. B. Maheran and O. Abu Zarin, “Felda’s Experience in Clonal Oil Palm Planting on Inland Soils,” Proceedings of the PORIM Oil Palm Congress, Kuala Lumpur, 1999, pp. 330-343.
[7] A. C. Soh, Y. Y. Yong, Y. W. Ho and N. Rajanaidu, “Commercial Potential of Oil Palm Clones: Early Results of Their Performances,” In: V. Rao, I. E. Henson and N. Rajanaidu, Eds., Recent Developments in Oil Palm Tissue Culture and Technology, Palm Oil Research Institute of Malaysia, Kuala Lumpur, 1995, pp. 134-144.
[8] A. C. Soh, G. Wong, C. C. Tan, P. S. Chew, T. Y. Hor, S. P. Chong and K. Gopal, “Recent Advances towards Com- Mercial Production of Elite Oil Palm Clones,” Proceedings of the MPOB 2001 International Palm Oil Congress―Agriculture, Kuala Lumpur, 2001, pp. 33-44.
[9] C. J. Eeuwens, S. Lord, C. R. Donough, V. Rao, G. Vallejo and S. Nelson, “Effects of Tissue Culture Condi- Tions during Embryoid Multiplication on the Incidence of ‘Mantled’ Flowering in Clonal Propagated Oil Palm,” Cell Plant, Tissue Organ Culture, Vol. 70, 2002, pp. 311-323. doi:10.1023/A:1016543921508
[10] S. Jouannic, X. Argout, F. Lechauve, C. Fizames, A. Borgel, F. Morcillo, F. Aberlec-Bertossi, Y. Duval and J. W. Tregear, “Analysis of Expressed Sequence Tags from Oil Palm (Elaeis guineensis),” FEBS Letters, Vol. 579, 2005, pp. 2709-2714. doi:10.1016/j.febslet.2005.03.093
[11] A. C. Soh, G. Wong, C. C. Tan, T. Y. Hor and C. K. Wong, “Revisited: Cloning Seedling of Reproduced Best Dxp Cross Strategy,” Paper Presented at ISOPB Seminar on the Progress of Oil Palm Breeding and Selection, Medan, 6-9 October 2003.
[12] S. Rajinder, N. Jayanthi, T. Soon-Guan, M. P. Jothi and S. C. Cheah, “Development of Simple Sequence Repeat (SSR) Markers for Oil Palm and Their Application in Genetic Mapping and Fingerprinting of Tissue Culture Clones,” Asia Pacific Journal of Molecular Biology and Biotechnology, Vol. 15, 2007, pp. 121-131.
[13] M. Delseny, M. Laroche and P. Penon, “Detection of Sequences with Z-DNA Forming Potential in High Plants,” Biochemical and Biosphysical Research Commununications, Vol. 116, 1983, pp. 113-120. doi:10.1016/0006-291X(83)90388-1
[14] W. Amos, S. J. Sawcer, R. W. Feakes and D. C. Rubinsztein, “Microsatellites Show Mutational Bias and Heterozygote Instability,” Nature Genetics, Vol. 13, 1996, pp. 390-391. doi:10.1038/ng0896-390
[15] J. S. C. Smith, E. C. L. Chin, H. Shu, O. S. Smith, S. J. Wall, M. L. Senior, S. E. Mitchel, S. Kresovich and J. Ziegle, “An Evaluation of the Utility of SSR Loci as Mo- Lecular Markers in Maize (Zea mays L.), Comparisons with Data from RFLPs and Pedigree,” Theoretical and Applied Genetics, Vol. 95, 1997, pp. 163-173. doi:10.1007/s001220050544
[16] J. Doyle and L. Doyle, “Isolation of Plant DNA from Fresh Tissue,” Focus, Vol. 12, 1990, pp. 13-15.
[17] N. Billotte, N. A. M. Risterucci, E. Barcelos, L. Noyer, P. Amblard and F. C. Baurens, “Development, Characterisa- tion, and Across-Taxa Utility of Oil Palm (Elaeis guineensis Jacq.) Microsatellite Markers,” Genome, Vol. 44, 2001, pp. 413-425.
[18] M. Nei and N. Takezaki, “Estimation of Genetic Distances and Phylogenetic Trees from DNA Analysis,” Proceedings of the 5th World Congress on Genetics. Applied to Livestock Production, Vol. 21, 1983, pp. 405- 412.
[19] P. H. A. Sneath and R. R. Sokal, “Numerical Taxonomy,” W. H. Freeman Press, San Francisco, 1973.
[20] B. S. Weir, “Genetic Data Analysis II,” MA: Sinauer As- sociates, Inc., Sunderland, 1996.
[21] M. Nei, “Analysis of Gene Diversity in Subdivided Populations,” Proceedings of the National Academy of Science of the USA, Vol. 70, 1973, pp. 3321-3323. doi:10.1073/pnas.70.12.3321
[22] K. Liu and S. V. Muse, “PowerMarker: Intergrated Analysis Environment for Genetic Marker Data,” Bioinformatics, Vol. 21, 2005, pp. 2128-2129. doi:10.1093/bioinformatics/bti282
[23] B. Cochard, “Etude de la Diversité Génétique et du Déséquilibre de Liaison au Sein de Populations Améliorées de Palmier à Huile (Elaeis guineensis Jacq.),” Ph.D. Dissertation, SUPAGRO, Montpellier, 2008.
[24] F. C. Yeh, “Population Genetics,” In: A. Young, D. Bosh- ier and T. Boyle, Eds., Forest Conservation Genetics: Principles and Practice, CSIRO Publishing, Collingwood, 2000, pp. 21-37.
[25] R. V. H. Corley, “Illegitimacy in Oil Palm Breeding,” Journal of Oil Palm Research, Vol. 17, 2005, pp. 64-69.
[26] N. Billotte, P. J. L. Lagoda, A. M. Risterucci and F. P. Baurens, “Microstellite-Enriched Libraries: Applied Methodology for the Development of SSR Markers in Tropical Crops,” Fruits, Vol. 54, 1999, pp. 277-288.
[27] C. Bakoumé, R. Wickneswari, N. Rajanaidu, A. Kushairi, P. Amblard and N Billotte, “Allelic Diversity of Natural Oil Palm (Elaeis guineensis Jacq.) Populations Detected by Microsatellite Markers: Implication in Conservation,” Plant Genetic Resources Characterization and Utilization, Vol. 5, 2007, pp. 104-107. doi:10.1017/S1479262107710870
[28] C. W. S. Hartley, “The Oil Palm (Elaeis guineensis Jacq.),” Longman Scientific and Technical, New York, 1988.
[29] C. Bakoumé, “Genetic Diversity of Natural Oil Palm (Elaeis guineensis Jacq.) Populations Using Microsatellite Markers,” unpublished Ph.D. dissertation, Universiti Kebangsaan Malaysia, Bangi, 2006.
[30] S. Diabate, H. De Franqueville, D. Allou, K. E. Konan, O. A. Coulibaly and W. P. N’Guessan, “Phenolic Diversity in the Defense Reaction of the Oil Palm against Vascular Wilt Disease,” Proceedings of the 2009 International Palm Oil Congress on Palm Oil: Balancing Ecologics with Economics, Kuala Lumpur, 2009, pp. 1027-1034.

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.