Trehalose and Sucrose Osmolytes Accumulated by Algae as Potential Raw Material for Bioethanol


Currently, obtaining sustainable fuels, such as biodiesel and bioethanol, from cheap and renewable materials is a challenge. In recent years, a new approach being developed consists of producing, sugars from algae by photosynthesis. Sugar accumulation can be increased under osmotic stress (osmoregulation). The aim of this study is to show the pro-duction of sugars from algae, isolated from natural sources, and the effect of osmotic stress on fermentable sugars ac-cumulation. Strain isolation, production of sugars from each alga and the effect of osmotic stress on growth and sugar production are described. Twelve algal strains were isolated, showing growths between 0.6 and 1.8 g of biomass dry weight /L, all with production of intracellular and extracellular sugars. The strain identified as Chlorella sp. showed an increase in sugar production from 23.64 to 421 mg of sugars/g of biomass dry weight after 24 h of osmotic stress with 0.4 M NaCl. Sucrose and trehalose, both fermentable sugars, were the compatible osmolytes accumulated in response to the osmotic stress. The isolated strains are potential producers of fermentable sugars, using the photosynthetic pathway and osmotic stress.

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M. Bremauntz, L. Torres-Bustillos, R. Cañizares-Villanueva, E. Duran-Paramo and L. Fernández-Linares, "Trehalose and Sucrose Osmolytes Accumulated by Algae as Potential Raw Material for Bioethanol," Natural Resources, Vol. 2 No. 3, 2011, pp. 173-179. doi: 10.4236/nr.2011.23023.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] E. Blumwald, R. Mehlhorn and L. Packer, “Studies of Osmoregulation in Salt Adaptation of Cyanobacteria with ESR Spin-Probe Techniques,” Proceedings of National Academy Sciences, Vol. 80, 1983, pp. 2599-2602. doi:10.1073/pnas.80.9.2599
[2] E. Elsheikh and W. Wood, “Rhizobia and Bradyrhizobiz under Salt Stress: Possible Role of Trehalose in Osmoregulation,” Letters Applied Microbiology, Vol. 10, 1990, pp. 127-129. doi:10.1111/j.1472-765X.1990.tb00098.x
[3] D. Los and N. Murata, “Membrane Fluidity and Its Role in the Perception of Environmental Signals,” Biochimica et Biophysica Acta, Vol. 1666, 2004, pp 142-157. doi:10.1016/j.bbamem.2004.08.002
[4] M. A. Luna-Velasco, F. Esparza-García, R. O. Ca?izares- Villanueva and R. Rodríguez-Vázquez, “Production and Properties of a Bioemulsifier Synthetsized by Phenanthrene-Degrading Penicillium sp,” Process Biochemisty, Vol. 42, 2007, pp. 310-314.
[5] M. C. Santiago-Santos, T. Ponce-Noyola, R. Olvera- Ramirez, J. Ortega-López and R. O. Ca?izares-Villanueva, “Extraction and Purification Phycocyanin from Calothrix sp.,” Process Biochemisty, Vol. 39, 2004, pp. 2047-2052.
[6] J. Batterton, C. Van Baalen, “Growth Responses of Blue-Green Algae to Sodium Chloride Concentration,” Archives Microbiology, Vol. 76, 1971, pp. 151-155. doi:10.1007/BF00411789
[7] R. Joset, M. Jeanjean, “Dynamics of the Response of Cyanobacteria to Salt-Stress: Deciphering the Molecular Events,” Physiol Plant, Vol. 96, 1996, pp. 738-744. doi:10.1111/j.1399-3054.1996.tb00251.x
[8] M. Page-Sharp, C. Behm and G. Smith, “Involvement of Compatible Solutes Trehalose and Sucrose in the Response to SALT Stress of Cyanobaterial Scytonema Species Isolated from Desert Solils,” Biochimica et Biophysica Acta, Vol. 1472, 1999, pp. 519-528.
[9] G. Stacey, C. Van Baalen and F. Tabita, “Isolation and Characterization of a Marine Anabaena sp. Capable of Rapid Growth on Molecular Nitrogen,” Archives of Microbiology, Vol. 144, 1977, pp. 197-201. doi:10.1007/BF00446862
[10] A. Porchia and G. Salerno, “Sucrose Biosynthesis in a Prokaryotic Organism: Presence of Two Sucrose-Pho- sphate Synthases in Anabaena with Remarkable Differences Compared with the Plant Enzymes,” Proceedings of National Academy Sciences, Vol. 93, 1996, pp. 13600-13604. doi:10.1073/pnas.93.24.13600
[11] National Water Commission Report, “Informe SU-6-C03 -3-390,” 1996.
[12] R. Rippka, J. Deruelles, J. Waterbury, M. Herdman and R. Stanier, “Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria,” Journal General Microbiology, Vol. 111, 1979, pp. 1-61.
[13] P. Bourrely, “Les Algues d′eau douce,” Editions N. Boubeé & Cie., Paris, 1966.
[14] J. Müller, C. Staehelin, R. Mellor, T. Boller and A. Wiemken, “Trehalose and Trehalase in Root Nodules from Various Legumes,” Physiologia Plantarum, Vol. 90, No. 1, 1994, pp. 86-92.
[15] L. Brown, “Photosynthetic and Growth Responses to Salinity in a Marine Isolated Nannochloris bacillaris (Chlorophyceae),” Journal of Phicology, Vol. 18, No. 4, 1982, pp. 483-488.
[16] R. Abed, K. Kohl and D. Beer, “Effect of Salinity Changes on the Bacterial Diversity Photosynthesis and Oxygen Consumption of Cyanobacterial Mats from an Intertidal of Arabian Gulf,” Environmental Microbiology, Vol. 9, No. 6, 2007, pp. 1384-1392. doi:10.1111/j.1462-2920.2007.01254.x
[17] L. Gustavs, A. Eggert, D. Michalik and U. Karten, “Physiological and Biochemical Responses of Green Microalgae from Different Habitats to Osmotic and Matric Stress,” Protoplasma, Vol. 243, No. 1-4, 2010, pp. 3-14.
[18] C. Colaco, C. Smith, S. Sen, D. Roser, Y. Newman, S. Ring, (eds.), “Formulation and Delivery of Proteins and Peptides. IV Series,” American Chemical Society, Washington, D. C., 1993, pp. 220-240.
[19] A. Oren,” Diversity of Organic Osmotic Compounds and Osmotic Adaptation in Cyanobacteria and Algae,” In: Seckback Ed., Algae and cyanobacteria in extreme environment, Springer, Berlin, 2007, pp. 639-655.
[20] A. Ben Amotz and M. Avron, “Accumulation of Metabo- lities by Halotolerant Algae and Its Industrial Potencial,” Annual Review Microbiology Vol. 37, 1983, pp. 95-119. doi:10.1146/annurev.mi.37.100183.000523
[21] A. Ben-Amotz and T. Grunwald, “Osmoregulation in the Halotolerant Algae Asteromonas gracilis,” Plant Physiolpgy, Vol. 67, 1981, pp. 613-616.
[22] Y. Chisti, “Biodiesel from Microalgae,” Biotechnology Advances, Vol. 25, No. 3, 2007, pp. 294-306. doi:10.1016/j.biotechadv.2007.02.001
[23] J. Zaldivar, J. Nielsen and L. Olsson, “Fuel Ethanol from Lignocelluloses: A Challenge for Methabolic Engineering and Process Integration,” Applied Microbiology Biotechnology, Vol. 56, No. 1-2, 2001, pp. 17-34. doi:10.1007/s002530100624
[24] C. Posten and G. Schaub, “Microalgae and Terrestrial Biomass as Source for Fuels—A Process View,” Journal of Biotechology, Vol. 142, No. 1, 2009, pp. 142: 64-69.
[25] R. Vazquez-Duhalt and B. Arredondo, “Haloadaptation of a Green Alga Botryococcus braunii (race A),” Phytochemistry, Vol. 30, No. 9, 1991, pp. 2919-2915.
[26] E. Visser, D. Filhi, M. Martins and B. Steward, “Bioethanol Production Potential from Brazilian Biodiesel Co-Products,” Biomass and Bioenergy, Vol. 35, 2011, pp. 489-494. doi:10.1016/j.biombioe.2010.09.009
[27] L. P. F. Carvalho, A. R. J. Cabrita, R. J. Dewhurst, T. E. J. Vicente, Z. M. C. Lopes and A. J. M. Fonseca, “Evaluation of Palm Kernel Meal and Corn Distillers Grains in Corn Silage-Based Diets for Lactating Dairy Cows,” Journal of Dairy Science, Vol. 89, No. 7, 2006, pp. 2705-2715.
[28] S. Yusoff, “Renewable Energy from Palm Oil-Innovation on Effective Utilization of Waste,” Journal of Cleaner Production, Vol. 14, No.1, 2006, pp. 87-93. doi:10.1016/j.jclepro.2004.07.005
[29] A. Córdova–Izquierdo, J. A. Lara Torres, R. Amaro Gutiérrez, S. D. Pe?a Betancourt and V. M. Xolalpa Campos, “Composition of Milk from Holstein Cows Supplemented with Sugar Cane Enriched Comprehensive Paperback Saccharina,” Revista veterinria, Vol. 1, No. 1, 2010, pp. 66-68.

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