The Function of miR-31 Expression in Differentiation of Neural Stem Cells

Xiao Li^1,2, Yuantao Gao³, Pengfei Li^1,2, Feng Tian^1,2, Jing Wang^1,2, Chunfang Wang^1,2

¹Experimental Animal Center, Shanxi Medical University, Shanxi, China.
²Shanxi Key Laboratory of Laboratory Animal and Animal Model of Human Diseases, Shanxi Medical University, Shanxi, China.
³Nanchang University Queen Mary School, Nanchang, China.

DOI: 10.4236/jbm.2016.43010                

Abstract

Spinal cord injury (SCI) is a severe complication of acute spinal injury, it can lead to axonal degeneration and necrosis of neurons, causing damage cross section below the movement, then loss of sensory, reflex and autonomous control function below the damage section. Nowadays, there is increasing evidence that transplantation of neural stem cells can help to repair spinal cord injury after spinal cord injury. The aim of this study was to research the function of miR-31 expression in differentiation of neural stem cells. Mir-31 was transfected the mimics and inhibitor into neural stem cells. By the morphological observation, the cell over expression of miR-31 was closer to NSCs. With the RT-PCR, Hb9 and STMN1 were up-regulate when miR-31 was inhibited, and Nestin was down-regulate. When miR-31 was over expression, the expression of Nestin and Hb9 was up-regulate. These findings suggest that miR-31 may play a significant role in proliferation and differentiation of neural stem cell and are potential targets for therapeutic interventions following spinal cord injury.

Keywords

Spinal Cord Injury, microRNA, Neural Stem Cells, Transplantation, Mouse

Conflicts of Interest

The authors declare no conflicts of interest.

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