GC-MS Analysis of the Essential Oil and Methanol Extract of the Seeds of Steganotaenia araliacea Hochst

Mussie Sium Demoz^1*, Kareru Patrick Gachoki¹, Keriko Joseph Mungai¹, Berhane Girmay Negusse²

¹Department of Chemistry, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
²Department of Chemistry, College of Health Sciences, Asmara, Eritrea.

DOI: 10.4236/ajps.2014.526392                

Abstract

Background: Steganotaenia araliacea is a multipurpose plant and has wider applications in the folklore for the treatment of various ailments. Previously the presence of antileukemic lignan lactones and saponins was detected in the stem bark, root and leaf of Steganotaenia araliacea. Besides, the diuretic and antibacterial activities of the plant were reported. However, there has been no attempt to examine the constituents of the seeds of Steganotaenia araliacea. This paper reports the first such study of both the essential oil and methanol extract of the seeds of the plant. Methods: The seeds of the plant were shade dried, pounded and thus extracted using methanol. Besides, the essential oil of the seeds was collected using steam distillation. The components of the methanol extract were studied both by GC-MS and preliminary phytochemical studies; the essential oil was running on GC-MS for analysis. Results: The GC-MS analysis of the essential oil of the seeds identified the presence of α-linalool, α-pinene, m-cresol, p-menth-1-en-4-ol, p-menth-1-en-8-ol, myristicin and others. Besides, the methanol extract of the seeds showed the presence of falcarinol, apiol, scoparone, stigmasterol, myristicin etc. The preliminary phytochemical analysis of the methanol extract of the seeds confirms the presence of alkaloids, flavonoids, tannins, coumarines, steroids, and phenols. Conclusion: This plant contains bioactive metabolites and thus can be used as an alternative and complementary medicine in treatment of different ailments. However, further studies on the bioactivity and toxicity of the plant should be done.

Keywords

GC-MS, Steganotaenia araliacea, Essential Oil, Phytochemicals

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Mabberley, D.J. (1997) The Plant-Book. 2nd Edition, Cambridge University Press, Cambridge, 680.
[2]	Bein, E., Habte, B., Jaber, A., Ann, B. and Tengnas, B. (1996) Useful Trees and Shrubs in Eritrea. Identification, Propagation and Management for Agricultural and Pastoral Communities. Regal Press Limited, Nairobi, 364.
[3]	Senai, W.A. (2010) Legislative Regulation of Traditional Medicinal Knowledge in Eritrea vis-à-vis Eritrea’s Commitments under the Convention on Biological Diversity: Issues and Alternatives. Law, Environment and Development Journal, 6/2, 130.
[4]	Addy, M.E. (2005) Western Africa Network of Natural Products Research Scientific Meeting. African Journal of Traditional, Complementary and Alternative Medicine, 2, 177-205.
[5]	Kupchan, S.M., Britton, R.W., Ziegler, M.F., Gilmore, C.J., Restivo, R.J. and Bryan, R.F. (1973) Tumor Inhibitors. Steganacin and Steganangin, Novel Antileukemic Lignan Lactones from Steganotaenia araliacea. Journal of American Chemical Society, 95, 1335-1336. http://dx.doi.org/10.1021/ja00785a054
[6]	Taafrout, M., Rouessac, F. and Robin, J.-P. (1983) Araliangine, a New Bisbenzocyclo-Octadienolactonic Lignan from Steganotaenia araliacea, Hochst. Tetrahedron Letter, 24, 197-200. http://dx.doi.org/10.1016/S0040-4039(00)81364-7
[7]	Taafrout, M., Rouessac, F. and Robin, J.-P. (1983) Neoisostegane, a New Bisbenzocyclo-Octadienolactonic Lignan from Steganotaenia araliacea, Hochst. Tetrahedron Letter, 24. 2983-2986. http://dx.doi.org/10.1016/S0040-4039(00)88075-2
[8]	Taafrout, M., Rouessac, F. and Robin, J.-P. (1983) Prestegane A. from Steganotaenia araliacea Hochst.: The First Natural Dibenzylbutanolide Lignan with a Meta-Phenol—A Short Synthesis of Cis- and Trans-(±)-Arctigenin. Tetrahe- dron Letter, 24, 3237-3238. http://dx.doi.org/10.1016/S0040-4039(00)88144-7
[9]	Hicks, R.P. and Sneden, A.T. (1983) Neoisostegane, a New Bis-Benzocyclooctadiene Lignan Lactone from Steganotaenia araliacea Hochst. Tetrahedron Letter, 24, 2987-2990.
[10]	Taafrout, M., Rouessac, F., Robin, J.-P., Hicks, R.P., Slillady, D.D. and Sneden, A.T. (1984) Neoisostegane, a New Bisbenzocyclooctadiene Lignan Lactone from Steganotaenia araliacea. Journal of Natural Products, 47, 600-606. http://dx.doi.org/10.1021/np50034a005
[11]	Robin, J.P., Davoust, D. and Taafrout, M. (1986) Steganolides B and C, New Lignans Analogous to Episteganacin and Isolated from Steganotaenia araliacea Hochst. Carbon-13-Proton Correlation of Staganacin Using Two-Dimensional NMR. Tetrahedron Letters, 27, 2871-2874. http://dx.doi.org/10.1016/S0040-4039(00)84666-3
[12]	Kapundu, M., Kakera, L.K., Graftieaux, A. and Delaude, C. (1987) Chemical Study of the Saponin from Steganotaenia araliacea Hoechst. Bulletin of Society Royal Science Liege, 56, 125-128.
[13]	Lavaud, C., Massiot, G., Le Men-Olivier, L., Viari, A., Vigny, P. and Delaude, C. (1992) Saponins from Steganotaenia araliacea. Phytochemistry, 31, 3177-3181. http://dx.doi.org/10.1016/0031-9422(92)83470-J
[14]	Wickramaratne, D.B.M., Pengsuparp, T., Mar, W., Chai, H.B., Chagwedera, T.E., Beecher, C.W.W., Farnsworth, N.R., Kinghorn, A.D., Pezzuto, J.M. and Cordell, G.A. (1993) Novel Antimitotic Dibenzocyclo-Octadiene Lignan Constituents of the Stem Bark of Steganotaenia araliacea. Journal of Natural Products, 56, 2083-2090. http://dx.doi.org/10.1021/np50102a009
[15]	Meragelman, K.M., McKee, T.C. and Boyd, M.R. (2001) 10-Demethoxystegane, a New Lignan from Steganotaenia araliacea. Journal of Natural Products, 64, 1480-1482. http://dx.doi.org/10.1021/np010248h
[16]	Hughes, L.R. and Raphael, R.A. (1976) Synthesis of the Antileukemic Lignan Precursor (±) Steganone. Tetrahedron Letters, 17, 1543-1546. http://dx.doi.org/10.1016/S0040-4039(00)71306-2
[17]	Robin, J.P., Gringore, O. and Brown, E. (1980) Asymmetric Total Synthesis of the Antileukemic Lignan Precursor (-)Steganone and Revision of Its Absolute Configuration. Tetrahedron Letters, 21, 2709-2712. http://dx.doi.org/10.1016/S0040-4039(00)78586-8
[18]	Taafrout, M., Rouessac, F., Robin, J.P. and Davoust, D. (1984) Isolation of Prestegane B, the First Bis-(m-hydroxy-denzyl)butanelide Lignan of Plant Origin, from Steganotaenia araliacea Hochst. Isolation and One-Step Synthesis of Dimethyl Matairesinol. Tetrahedron Letters, 25, 4127-4128. http://dx.doi.org/10.1016/S0040-4039(01)90199-6
[19]	Taafrout, M., Landais, Y., Robin, J.P. and Davoust, D. (1986) Isolation, Stereochemical Study, and Biomimetic Synthesis of Steganolide A, a New Dibenzocyclooctadieno-Lactone Lignan of Steganotaenia araliacea. Tetrahedron Letters, 27, 1781-1784. http://dx.doi.org/10.1016/S0040-4039(00)84373-7
[20]	Ojerinde, S.O., Adepoju, B.A., Edache, J.J., Okundaye, E.M. and Alemika, T.E. (2001) Antioxidant and Antibacterial Constituents of Steganotaenia araliacea Stem Bark. Journal of Pharmacy & Bioresources, 10, 25-32.
[21]	Lino, A. and Deogracious, O. (2006) The in Vitro Antibacterial Activity of Annona senegalensis, Securidacca longi- pendiculata and Steganotaenia araliacea. African Journal of Health Sciences, 6, 31-35.
[22]	Agunu, A., Abdurahman, E.M., Andrew, G.O. and Muhammed, Z. (2005) Diuretic Activity of the Stem-Bark Extracts of Steganotaenia araliacea Hochst [Apiaceae]. Journal of Ethnopharmacology, 96, 471-475. http://dx.doi.org/10.1016/j.jep.2004.09.045
[23]	Wang, R.W., Rebhum, L.I. and Kupchan, S.M. (1977) Antimitotic and Antitubulin Activity of the Tumor Inhibitor Steganacin. Cancer Research, 37, 3071-3079.
[24]	NjanNloga, A.M., Ngo Yebga, J. and Ngo Bum, E. (2014) Antiplasmodial Effect of Commelina benghalensis/Stegano-taenia araliacea Plants Extract on the Human Population in Ngaoundere (Cameroon). Journal of Medical Sciences, 14, 68-74. http://dx.doi.org/10.3923/jms.2014.68.74
[25]	Abubakar, M.S., Abdurahman, E.M., Nock, I.H., Haruna, A.K. and Garba, M. (2001) The Evaluation of Pest Control Properties of Steganotaenia araliacea. Journal of Herbs, Spices and Medicinal Plants, 8, 51-57. http://dx.doi.org/10.1300/J044v08n01_07
[26]	Ndjonka, D., Agyare, C., Luersen, K., Hensel, A. and Liebau, E. (2010) In Vitro Anti-Leishmanial Activity of Traditional Medicinal Plants from Cameroon and Ghana. International Journal of Pharmacology, 6, 863-871. http://dx.doi.org/10.3923/ijp.2010.863.871
[27]	Trease, G.E. and Evans, W.C. (1989) Pharmacognosy. 11th Edition, Bailliere Tindall, London, 45-50.
[28]	Harbone, J.B. (1998) Methods of Extraction and Isolation. In: Phytochemical Methods, Chapman and Hall, London, 60-66.
[29]	Noudjou, F., Ngassoum, M.B., Mapongmetsem, P.M., Marlier, M., Verscheure, M. and Lognay, G.C. (2006) Analysis by GC/FID and GC/MS of Essential Oil of Leaflets of Steganotaenia araliacea Hochst from Cameroon. Journal of Essential Oil Research, 18, 305-307. http://dx.doi.org/10.1080/10412905.2006.9699097
[30]	Bakkali, F., Averbeck, A., Averbeck, D. and Idaomer, M. (2008) Biological Effects of Essential Oils—A Review. Food and Chemical Toxicology, 46, 446-475. http://dx.doi.org/10.1016/j.fct.2007.09.106
[31]	Ciganda, C. and Laborde, A. (2003) Herbal Infusions Used for Induced Abortion. Clinical Toxicology, 41, 235-239. http://dx.doi.org/10.1081/CLT-120021104
[32]	Fang, Y., Li, Z. and Watanabe, Y. (2003) Pharmacokinetics of a Novel Anti-Asthmatic Scoparone, in the Rabbit Serum Assessed by a Simple HPLC Method. Journal of Ethnopharmacology, 86, 127-130. http://dx.doi.org/10.1016/S0378-8741(03)00039-4
[33]	Purup, S., Larsen, E. and Christensen, L.P. (2009) Differential Effects of Falcarinol and Related Aliphatic C17-Poly-acetylenes on Intestinal Cell Proliferation. Journal of Agricultural and Food Chemistry, 57, 8290-8296. http://dx.doi.org/10.1021/jf901503a
[34]	Kobaek-Larsen, M., Christensen, L.P., Vach, W., Ritskes-Hoitinga, J. and Brandt, K. (2005) Inhibitory Effects of Feeding with Carrots or (-)-Falcarinol on Development of Azoxymethane-Induced Preneoplastic Lesions in the Rat Colon. Journal of Agricultural and Food Chemistry, 53, 1823-1827. http://dx.doi.org/10.1021/jf048519s
[35]	Ray, A. (2005) Review Article: Cancer Preventive Role of Selected Dietary Factors. Indian Journal of Cancer, 42, 15- 24. http://dx.doi.org/10.4103/0019-509X.15095