Enhancement of Gingival Wound Healing by Local Application of Silver Nanoparticles Periodontal Dressing Following Surgery: A Histological Assessment in Animal Model


Background: The value of periodontal dressing and its effects on periodontal wound healing have been questioned. Besides, the early healing of the gingival and periodontal wounds in oral cavity should be emphasized to promote the treatment outcome. Several studies pointed out a potential positive effect of silver nanoparticles (NPs) on the healing process of skin wounds. The present work aimed to design silver NPs periodontal dressing and then, evaluated its effects on gingival wound healing following operation. Methods: Thirty healthy adolescent female rabbits were used in this study. They had intact teeth and the clinical and radiographic examination revealed no periodontal disease. Silver NPs were added to carbonate methyl cellulose as base with two different concentrations of 25% v/v (Group A) and 50% v/v (Group B). On the basis of simple randomized allocation, gingivectomy was performed at the buccal gingiva of the left and right mandibular incisors and subsequently, untreated wounds in the control area were covered with periodontal pack and periodontal dressings A and B were applied in the test regions. Histological changes were monitored in day 4 and 7 post operation to evaluate the inflammatory and repair stage of healing process. Results: We observed significant difference in the inflammatory and repair parameters of the healing process between surgical sites treated with this periodontal dressing and control group. Besides, wounds treated with silver NPs dressing in general terms had all the hallmarks of relatively complete healing one week post operatively. Conclusion: The results indicated positive potential therapeutic effects for this new formulation of periodontal dressing on acceleration of surgical wound healing that led to improvement of periodontal treatment consequences following operation.

Share and Cite:

Habiboallah, G. , Mahdi, Z. , Majid, Z. , Nasroallah, S. , Taghavi, A. , Forouzanfar, A. and Arjmand, N. (2014) Enhancement of Gingival Wound Healing by Local Application of Silver Nanoparticles Periodontal Dressing Following Surgery: A Histological Assessment in Animal Model. Modern Research in Inflammation, 3, 128-138. doi: 10.4236/mri.2014.33016.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Cheng, D., Yang, J. and Zhao, Y. (2004) Antibacterial Materials of Silver Nanoparticles Application in Medical Appliances and Appliances for Daily Use. Chinese Medical Equipment Journal, 4, 26-32.
[2] Zhang, Z., Yang, M., Huang, M., Hu, Y. and Xie, J. (2007) Study on Germicidal Efficacy and Toxicity of Compound Disinfectant Gel of Nanometer Silver and Chlorhexidineacelate. Chinese Journal of Health Laboratory Technology, 17, 1403-1406.
[3] Cohen, M.S., Stern, J.M., Vanni, A.J., Kelley, R.S., Baumgart, E., Field, D., Libertino, J.A. and Summerhayes, I.C. (2007) In Vitro Analysis of a Nanocrystalline Silver-Coated Surgical Mesh. Surgical Infections, 8, 397-403. http://dx.doi.org/10.1089/sur.2006.032
[4] Chaloupka, K., Malam, Y. and Seifalian, A.M. (2010) Nanosilver as a New Generation of Nanoproduct in Biomedical Applications. Trends in Biotechnology, 28, 580-588.
[5] Sharma, V.K., Yngard, R.A. and Lin, Y. (2009) Silver Nanoparticles: Green Synthesis and Their Antimicrobial Activities. Advances in Colloid and Interface Science, 145, 83-96.
[6] Newman, M. and Carranza, F. (2006) Carranza’s Clinical Periodontology. 10th Edition, Saunders Co., Lindo.
[7] Fong, J. and Wood, F. (2006) Nanocrystalline Dressings in Wound Management, a Review. International Journal of Nanomedicine, 1, 441-449. http://dx.doi.org/10.2147/nano.2006.1.4.441
[8] Alt, V., Bechert, T., Steinrücke, P., Wagener, M., Seidel, P., Dingeldein, E., Domann, E. and Schnettler, R. (2004) An in Vitro Assessment of the Antibacterial Properties and Cytotoxicity of Nanoparticulate Silver Bone Cement. Biomaterials, 25, 4383-4391.
[9] Fuj, J.I.J., Fan, D. and Shen, J. (2007) Construction of Antibacterial Multilayer Films Containing Nanosilvervia Layer-by-Layer Assembly of Heparin and Chitosen-Silver Ions Complex. Journal of Biomedical Materials Research, 790, 665-674.
[10] Castellano, J.J., Shafii, S.M., Ko, F., Donat, G., Wright, T.E., Mannavi, R.J., Payne, W.G., Smith, D.J. and Robson, M.C. (2007) Comparative Evaluation of Silver-Containing Antimicrobial Dressings and Drugs. International Wound Journal, 4, 114-122. http://dx.doi.org/10.1111/j.1742-481X.2007.00316.x
[11] Min, S., Gao, X., Han, C., Chen, Y., Yang, M., Zhu, L., Zhang, H., Liu, L. and Yao, J. (2012) Preparation of a Silk Fibroin Spongy Wound Dressing and Its Therapeutic Efficiency in Skin Defects. Journal of Biomaterials Science, Polymer Edition, 23, 97-110.http://dx.doi.org/10.1163/092050610X543609
[12] Zhao, L., Zhang, W.X., Chen, D.L., Qiu, H. and Li, M. (2013) Function Model Construction on the Question of Defining Optimal Ag-Concentration in Silver-Containing Biological Wound Dressing. Composite Interfaces, 20, 139-153. http://dx.doi.org/10.1080/15685543.2013.764510
[13] Singh, R. and Singh, D. (2012) Radiation Synthesis of PVP/Alginate Hydrogel Containing Nanosilver as Wound Dressing. Journal of Materials Science: Materials in Medicine, 23, 2649-2658.
[14] Sachs, H.A., Farnoush, A., Checchi, L. and Joseph, C.E. (1984) Current Status of Periodontal Dressing. Journal of Periodontology, 55, 689-696. http://dx.doi.org/10.1902/jop.1984.55.12.689
[15] Smeekens, J.P., Maltha, J.C. and Renggli, H.H. (1992) Histological Evaluation of Surgically Treated Oral Tissues after Application of Photocuring Periodontal Dressing Material: An Animal Study. Journal of Clinical Periodontology, 19, 641-645. http://dx.doi.org/10.1111/j.1600-051X.1992.tb01712.x
[16] Wampole, H.S., Allen, A.L. and Gross, A. (1978) The Incidence of Transient Bacteremia during Periodontal Dressing Change. Journal of Periodontology, 49, 462-468.
[17] Nezwek, R.A., Caffesse, R.G., Bergenholtz, A. and Nasjleti, C.E. (1980) Connective Tissue Response to Periodontal Pack. Journal of Periodontology, 51, 521-529.
[18] Scardino, M.E., Swaim, S.F., Strain, E.A., Hoffman, C.E., Oligive, G.K., Hanson, R.A., et al. (1999) The Effect of Omega-3 Fatty Acid Diet Enrichment on Wound Healing. Veterinary Dermatology, 10, 283-290. http://dx.doi.org/10.1046/j.1365-3164.1999.00148.x
[19] Martin, P., Hopkinson-Wooley, J. and McClusky, J. (1992) Growth Factor and Cutanous Wound Repair. Progress in Growth Factor Research, 4, 25-44. http://dx.doi.org/10.1016/0955-2235(92)90003-Z
[20] Wilkinson, L.J., White, J.K. and Chipman, J.K. (2011) Silver and Nanoparticles of Silver in Wound Dressings: A Review of Efficacy and Safety. Journal of Wound Care, 20, 543-549.
[21] Pesson, G. and Thilander, H. (1968) Experimental Studies of Surgical Packs. In Vitro Experiments on Antimicrobial Effect. Odontol Tidskr, 76, 147-155.
[22] Heaney, T.G. and Appleton, J. (1976) The Effect of Periodontal Dressings on the Healthy Periodontium. Journal of Clinical Periodontology, 3, 66-76.
[23] Trop, M., Novak, M., Rodl, S., Hellbom, B., Kroell, W. and Goessler, W. (2006) Silver Coated Dressing Acticoat Caused Raised Liver Enzymes and Argyria-Like Symptoms in Burn Patient. Journal of Trauma-Injury Infection & Critical Care, 60, 648-652. http://dx.doi.org/10.1097/01.ta.0000208126.22089.b6
[24] Woodward, M. (2005) Silver Dressings in Wound Healing: What Is the Evidence? Primary Intention, 13, 153-160.
[25] Johnston, H.J., Hutchison, G., Christensen, F.M., Peters, S., Hankin, S. and Stone, V. (2010) A Review of the in Vivo and in Vitro Toxicity of Silver and Gold Particulates: Particle Attributes and Biological Mechanisms Responsible for the Observed Toxicity. Critical Reviews in Toxicology, 40, 328-346. http://dx.doi.org/10.3109/10408440903453074
[26] Tredget, E.E., Shankowsky, R., Groenveld, A. and Burrell, R. (1998) A Matched-Pair, Randomized Study Evaluating the Efficacy and Safety of Acticoat Silver-Coated Dressing for the Treatment of Burn Wounds. Journal of Burn Care & Rehabilitation, 19, 531-537. http://dx.doi.org/10.1097/00004630-199811000-00013
[27] Voight, D. and Paul, C. (2001) The Use of Acticoat as Silver Impregnated Telfa Dressings in a Regional Burn and Wound Care Center: The Clinicians View. Wounds, 13, 11-20.
[28] Tian, J., Wong, K.K., Ho, C.M., Lok, C.N., Yu, W.Y., Che, C.M., Chiu, J.F. and Tam, P.K. (2007) Topical Delivery of Silver Nanoparticles Promotes Wound Healing. ChemMedChem, 2, 129-136.
[29] Hollinger, M.A. (1996) Toxicological Aspects of Topical Silver Pharmaceuticals. Critical Reviews in Toxicology, 26, 255-260. http://dx.doi.org/10.3109/10408449609012524
[30] Hidalgo, E. and Dominguez, C. (1998) Study of Cytotoxicity Mechanisms of Silver Nitrate in Human Dermal Fibroblasts. Toxicology Letters, 98, 169-179.
[31] Ip, M., Lui, S.L., Poon, V.K., Lung, I. and Burd, A. (2006) Antimicrobial Activities of Silver Dressings: An in Vitro Comparison. Journal of Medical Microbiology, 55, 59-63.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.