Diversity of Secondary Metabolites from Two Antarctic Microbes Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011

Cheng Wang, Xiaoqing Tian, Qiao Yang, Yanan Lu, Liyan Ma, Hongliang Huang, Chengqi Fan

Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China; Shanghai Ocean University, Shanghai, China.

DOI: 10.4236/ojms.2014.43020                

Abstract

Diversity analysis on secondary metabolites of Antarctic microbes, Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011, together with the structural elucidation of some purified compounds, has been carried out for understanding of their chemical constituents. The methanol extracts of Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011 were subjected to HPLC-TOF MS test for diversity analysis on secondary metabolites, respectively. The chemical constituents of NJ-011 are mainly N-containing compounds including some alkaloids and short polypeptides, while those of NJ-008 are not N-containing ones. Three compounds were also isolated and identified from extract of NJ011 by different column chromatography and preparative HPLC, and their structures were elucidated as b-carboline (1), 3-benzylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione (2) and 3-isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione (3) by comparison of TOF MS, ¹Hand ¹³C-NMR data with those reported. More microbial material of Pseudomonas sp. NJ-011 should be needed for exploration of the minor constituents with complicated structures.

Keywords

Antarctic Microbes, Rhodococcus sp. NJ-008, Pseudomonas sp. NJ-011, Secondary Metabolite

Conflicts of Interest

The authors declare no conflicts of interest.

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