Diversity of Secondary Metabolites from Two Antarctic Microbes Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011


Diversity analysis on secondary metabolites of Antarctic microbes, Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011, together with the structural elucidation of some purified compounds, has been carried out for understanding of their chemical constituents. The methanol extracts of Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011 were subjected to HPLC-TOF MS test for diversity analysis on secondary metabolites, respectively. The chemical constituents of NJ-011 are mainly N-containing compounds including some alkaloids and short polypeptides, while those of NJ-008 are not N-containing ones. Three compounds were also isolated and identified from extract of NJ011 by different column chromatography and preparative HPLC, and their structures were elucidated as b-carboline (1), 3-benzylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione (2) and 3-isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione (3) by comparison of TOF MS, 1Hand 13C-NMR data with those reported. More microbial material of Pseudomonas sp. NJ-011 should be needed for exploration of the minor constituents with complicated structures.

Share and Cite:

Wang, C. , Tian, X. , Yang, Q. , Lu, Y. , Ma, L. , Huang, H. and Fan, C. (2014) Diversity of Secondary Metabolites from Two Antarctic Microbes Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011. Open Journal of Marine Science, 4, 214-220. doi: 10.4236/ojms.2014.43020.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Li, L.Y., Li, D.H., Luan, Y.P., Gu, Q.Q. and Zhu, T.J. (2012) Cytotoxic Metabolites from the Antarctic Psychrophilic Fungus Oidiodendron truncatum. Journal of Natural Products, 75, 920-927.
[2] Wu, G.W., Ma, H.Y., Zhu, T.J., Li, J., Gu, Q.Q. and Li, D.H. (2012) Penilactones A and B, Two Novel Polyketides from Antarctic Deep-Sea Derived Fungus Penicillium crustosum PRB-2. Tetrahedron, 68, 9745-9749.
[3] Ren, J.W., Xue, C.M., Tian, L., Xu, M.J., Chen, J., Deng, Z.W., Proksch, P. and Lin, W.H. (2009) Asperelines A-F, Peptaibols from the Marine-Derived Fungus Trichoderma asperellum. Journal of Natural Products, 72, 1036-1044.
[4] Ma, H.Y., Li, D.H., Gu, Q.Q. and Zhu, T.J. (2011) Bioactive Secondary Metabolites Produced by an Antarctic MarineDerived Fungus Penicillium chrysogenum PR4-1-3. Chinese Journal of Marine Drugs, 30, 18-24.
[5] Ren, H., Cao, X.L. and Gu, Q.Q. (2010) Antitumor Metabolites from Marine-Derived Fungus Gliocladium Catenulatum T31. Chinese Pharmaceutical Journal, 45, 1720-1723.
[6] Kearns, P.S. and Rideout, J.A. (2008) Nonsymmetrical β-Carbolinedimers from an Ascidian, Didemnum sp. Journal of Natural Products, 71, 1280-1282.
[7] Li, D.H., Gu, Q.Q., Zhu W.M., Liu, H.B., Fang, Y.C. and Zhu, T.J. (2005) Antitumor Components from Marine Actinomycete 11014 I: Cyclic Dipeptides. Chinese Journal of Antibiotics, 30, 449-452.
[8] Qi, S.H., Qian, P.Y. and Zhang, S. (2009) Antibacterial Metabolites from Marine Bacterium Pseudomonas sp. Natural Product Research and Development, 21, 420-423.
[9] Kong F.M., Singh, M.P. and Carter, G.T. (2005) Pseudopyronines A and B, α-Pyrones Produced by a Marine Pseudomonas sp. F92S91, and Evidence for the Conversion of 4-Hydroxy-α-pyrone to 3-Furanone. Journal of Natural Products, 68, 920-923.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.