Isolation and Characterization of Novel Microsatellite Markers in Walnut (Juglans regia L.)

Fatemeh Najafi, Mohsen Mardi, Baratali Fakheri, Seyed Moustafa Pirseyedi, Nafiseh Mehdinejad, Maryam Farsi

Agricultural Biotech- nology Research Institute of Iran (ABRII), Karaj, Iran.
Department of Agronomy and Plant Breeding, Faculty of Agri-culture, University of Zabol, Zabol, Iran.
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zabol, Zabol, Iran; Agricultural Biotech- nology Research Institute of Iran (ABRII), Karaj, Iran.
Faculty of Agriculture, University of Tehran, Karaj, Iran.

DOI: 10.4236/ajps.2014.53054                

Abstract

Thirteen microsatellite markers were isolated and characterized from Juglans regia L. Successful polymorphism of the 13 primer pairs was observed in various genotypes of J. regia. The number of polymorphic alleles ranged from 2 to 4 (with an average of 4.35). The polymorphic information content values ranged from 0.47 to 0.88 (with an average of 0.69). TC/AG and GAA/CTT class of repeats were the most abundant di-nucleotide and tri-nucleotide repeats, respectively.

Keywords

Isolation; Microsatellite; Juglans regia L.; Polymorphism; SSR Markers

Conflicts of Interest

The authors declare no conflicts of interest.

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