Hypocholesterolemic and Growth Promoting Effects of Lactobacillus plantarum AK Isolated from a Nigerian Fermented Cereal Product on Rats Fed High Fat Diet


The hypocholesterolemic effect of Lactobacillus plantarum AK strain was tested in rats fed high cholesterol diet. The lipid profile, growth rate and microflora dynamics of rats that received L. plantarum AK strain were significantly different from the control group (p < 0.5). In particular low density lipoprotein was lower in serum of rats that received L. plantarum than those of the control group. The test group had a higher growth rate, higher lactobacilli counts but lower coliform and Escherichia coli counts over a period of 40 days and these observed differences were significant (p < 0.05). These results are indicative of the abilities of this strain to lower serum cholesterol levels as well as to improve intestinal microbial balance as observed in the microflora dynamics over a period of 40 days.

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B. Akanbi and O. Agarry, "Hypocholesterolemic and Growth Promoting Effects of Lactobacillus plantarum AK Isolated from a Nigerian Fermented Cereal Product on Rats Fed High Fat Diet," Advances in Microbiology, Vol. 4 No. 3, 2014, pp. 160-166. doi: 10.4236/aim.2014.43021.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] R. Deng, “Food and Food Supplements with Hypocholesterolemic Effects,” Recent Patents on Food, Nutrition & Agriculture, Vol. 1, No. 1, 2009, pp. 15-24.
[2] D. Steinberg, “Thematic Review Series: The Pathogenesis of Atherosclerosis. An Interpretive History of the Cholesterol Controversy: Part II: The Early Evidence Linking Hypercholesterolemia to Coronary Disease in Humans,” Journal of Lipid Research, Vol. 46, No. 2, 2005, pp. 179-190.
[3] C. Ballantyne, B. Arroll and J. Shepherd, “CVD Management: Towards a Global Consensus,” European Heart Journal, Vol. 22, No. 21, 2005, pp. 2224-2231.
[4] J. Lee, Y. Kim, H. S. Yun, J. G. Kim, S. Oh and S. H. Kim, “Genetic and Proteomic Analysis of Factors Affecting Serum Cholesterol Reduction by Lactobacillus acidophilus A4,” Applied and Environmental Microbiology, Vol. 76, No. 14, 2010, pp. 4829-4835.
[5] B. Isomaa, P. Almgren, T. Tuomi, G. Forsen, K. Lahti, M. Nissen, M. Taskinen and L. Groop, “Cardiovascular Morbidity and Mortality Associated with the Metabolic Syndrome,” Diabetes Care, Vol. 24, No. 4, 2001, pp. 683-689. http://dx.doi.org/10.2337/diacare.24.4.683
[6] L. Kritharides, “Reducing Low-Density Lipoprotein Cholesterol-Treating to Target and Meeting New European Goals,” European Heart Journal, Vol. 6, 2004, pp. A12-A18.
[7] V. Sblendorio and B. Palmieri, “Accuracy of Analyses for Lipid Profile Parameters as Measured with the CR3000 System,” European Review for Medical and Pharmacological Sciences, Vol. 12, No. 3, 2008, pp. 191-196.
[8] B. J. Arsenault, J. S. Rana, E. S. G. Stroes, J. Després, P. K. Shah, J. J. P. Kastelein, N. J. Wareham, S. M. Boekholdt and K. Khaw, “Beyond Low-Density Lipoprotein Cholesterol: Respective Contributions of Non-High-Density Lipoprotein Cholesterol Levels, Triglycerides, and the Total Cholesterol/High-Density Lipoprotein Cholesterol Ratio to Coronary Heart Disease Risk in Apparently Healthy Men and Women,” Journal of the American College of Cardiology, Vol. 55, No. 1, 2010, pp. 35-41.
[9] A. G. Shaper, K. W. Jones and J. Kyobe, “Serum Lipids in the Three Nomadic Tribes of Northern Kenya,” The American Journal of Clinical Nutrition, Vol. 13, 1963, pp. 135-146.
[10] G. V. Mann and A. Spoerry, “Studies of a Surfactant and Cholesteremia in the Maasai,” The American Journal of Clinical Nutrition, Vol. 27, No. 5, 1974, pp. 464-469.
[11] M. Saarela, G. Mogensen, R. Fonden, J. Matto and T. Mattila-Sandholm, “Probiotic Bacteria: Safety, Functional and Technological Properties,” Journal of Biotechnology, Vol. 84, No. 3, 2000, pp. 197-215.
[12] G. Zoumpopoulou, B. Foligne, K. Christodoulou, C. Grangette, B. Pot and E. Tsakalidou, “Lactobacillus fermentum ACA-DC 179 Displays Probiotic Potential in Vitro and Protects against Trinitrobenzene Sulfonic Acid (TNBS)Induced Colitis and Salmonella Infection in Murine Models,” International Journal of Food Microbiology, Vol. 121, No. 1, 2008, pp. 18-26.
[13] Y. Wang, N. Xu, A. Xi, Z. Ahmed, B. Zhang and X. Bai, “Effects of Lactobacillus plantarum MA2 Isolated from Tibet Kefir on Lipid Metabolism and Intestinal Microflora of Rats Fed on High-Cholesterol Diet,” Applied Microbiology and Biotechnology, Vol. 84, No. 2, 2009, pp. 341-347.
[14] T. Asahara, K. Shimizu, T. Takada, S. Kado, N. Yuki, M. Morotomi, R. Tanaka and K. Nomoto, “Protective Effect of Lactobacillus caseistrain Shirota against Lethal Infection with Multi-Drug Resistant Salmonella entericaserovar Typhimurium DT104 in Mice,” Journal of Applied Microbiology, Vol. 110, No. 1, 2010, pp. 163-173.
[15] C. Chiu, T. Lu, Y. Tseng and T. Pan, “The Effects of Lactobacillus-Fermented Milk on Lipid Metabolism in Hamsters Fed on High-Cholesterol Diet,” Applied Microbiology and Biotechnology, Vol. 71, No. 2, 2006, pp. 238-245. http://dx.doi.org/10.1007/s00253-005-0145-0
[16] G. Jahreisa, H. Vogelsangb, G. Kiesslinga, R. Schuberta, C. Buntec, W. P. Hammes, “Infuence of Probiotic Sausage (Lactobacillus paracasei) on Blood Lipids and Immunological Parameters of Healthy Volunteers,” Food Research International, Vol. 35, No. 2-3, 2002, pp. 133-138.
[17] I. De Smet, P. De Boever and W. Verstraete, “Cholesterol Lowering in Pigs through Enhanced Bacterial Bile Salt Hydrolase Activity,” British Journal of Nutrition, Vol. 79, No. 2, 1998, pp. 185-194.
[18] J. Lovegrove and K. Jackson, “Coronary Heart Disease,” In: T. Mattila Sandholm and M. Saarela, Eds., Functional Dairy Products, Woodhill Publishing, Cambridge, 2003, pp. 54-87.
[19] D. I. A. Pereira and G. R. Gibson, “Effects of Consumption of Probiotics and Prebiotics on Serum Lipid Levels in Humans,” Critical Reviews in Biochemistry and Molecular Biology, Vol. 37, No. 4, 2002, pp. 259-281.
[20] M. P. St-Onge, E. R. Farnworth and P. J. H. Jones, “Consumption of Fermented and Unfermented Dairy Products: Effects on Cholesterol Concentrations and Metabolism,” The American Journal of Clinical Nutrition, Vol. 71, No. 3, 2000, pp. 674-681.
[21] T. M. S. Wolever, J. Fernandes and A. Veketeshwer Rao, “Serum Acetate: Propionate Ratio Is Related to Serum Cholesterol in Men but Not in Women,” The Journal of Nutrition, Vol. 126, No. 11, 1996, pp. 2790-2797.
[22] L. Wang, K. Liu, D. Gao and J. Hao, “Protective Effects of Two Lactobacillus plantarum Strains in Hyperlipidemic Mice,” World Journal of Gastroenterology, Vol. 19, No. 20, 2013, pp. 3150-3156.
[23] N. Xie, Y. Cui, Y. Yin, X. Zhao, J. Yang, Z. Wang, N. Fu, Y. Tang, X. Wang, X. Liu, C. Wang and F. Lu, “Effects of Two Lactobacillus Strains on Lipid Metabolism and Intestinal Microflora in Rats Fed a High-Cholesterol Diet,” BMC Complementary and Alternative Medicine, Vol. 11, No. 53, 2011, pp. 1-11.
[24] Y. Huang, F. Wu, X. Wang, Y. Sui, L. Yang and J. Wang, “Characterization of Lactobacillus plantarum Lp27 Isolated from Tibetan Kefir Grains: A Potential Probiotic bacterium with Cholesterol-Lowering Effects,” Journal of Dairy Science, Vol. 96, No. 5, 2013, pp. 2816-2825.
[25] K. Itoh, T. Ishii, N. Wakabayashi and M. Yamamoto, “Regulatory Mechanisms of Cellular Response to Oxidative Stress,” Free Radical Research, Vol. 31, No. 4, 1999, pp. 319-324.

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