Identification of F1 Cassava (Manihot esculenta Crantz) Progeny Using Microsatellite Markers and Capillary Electrophoresis


Generation of genetic diversity is necessary in improving on the potential of cassava when faced with various biotic and abiotic challenges. Presently, cassava breeders are breeding for a number of traits, such as drought tolerance, early root bulking, yield, starch, beta-carotene, protein, dry matter, pest and disease resistance, by relying on genetic diversity that exists in manihot esculenta germplasm. Controlled pollination is one of the main methods used to generate genetic diversity in cassava. However, the process of controlled pollination especially in an open field is prone to contamination by illegitimate pollen right from the time of pollination, seed collection, nursery bed establishment to planting of the trials. Therefore, authentication of the progeny obtained from cassava crosses is very important for genetic studies. Twelve informative microsatellite markers were used to verify the authenticity of 364 F1 progeny thought to come from four controlled parental crosses. The transmission of each allele at nine microsatellite loci was tracked from parents to progeny in each of the four Namikonga-derived F1 cassava families. Out of the 364 F1 progeny, 317 (87.1%) were true-to-type, 44 (12.1%) were a product of self-pollination and 3 (0.8%) were a product of open pollination. The consistency of the results obtained using microsatellite markers makes this technique a reliable tool for assessing the purity of progeny generated from cassava crosses.

Share and Cite:

K. Vincent, K. Robert, F. Morag, K. Tadeo, B. Yona and V. Peter, "Identification of F1 Cassava (Manihot esculenta Crantz) Progeny Using Microsatellite Markers and Capillary Electrophoresis," American Journal of Plant Sciences, Vol. 5 No. 1, 2014, pp. 119-125. doi: 10.4236/ajps.2014.51015.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] FAOSTAT, “Cassava Production,” 2013.
[2] FAO, “Cassava, a 21st Century Crop,” Save and Grow Cassava; A Guide to Sustainable Production Intensification, Food and Agriculture Organization of the United Nations, Rome, 2013, pp. 1-18.
[3] FAO, “Why Cassava?” 2008.
[4] New Agriculturist, “Green Light for Yellow Cassava,” 2012.
[5] N. Nagib and O. Rodomiro, “Breeding Cassava to Feed the Poor,” Scientific American, 2010, pp. 78-84.
[6] R. Corley, “Illegitimacy in Oil Palm Breeding,” Journal of Oil Palm Research, Vol. 17, 2005, pp. 64-69.
[7] D. P. Khasa, S. Nadeem, B. Thomas, A. Robertson and J. Bousquet, “Application of SSR Markers for Parentage Analysis of Populus Clones,” Forest Genetics, Vol. 10, No. 4, 2003, pp. 73-281.
[8] M. Ferguson, J. Sarah, J. Timothy, W. Steve, D. Christopher, Y. Joe, P. Marri, R. Ismail and P. Etienne, “Identification, Validation and High-Throughput Genotyping of Transcribed Gene SNPs in Cassava,” Theoretical and Applied Genetics, Vol. 124, 2011, pp. 685-695.
[9] S. Riaz, S. Vezzulli, E. Harbertson and M. A. Walker, “Use of Molecular Markers to Correct Grape Breeding Errors and Determine the Identity of Novel Sources of Resistance to Xiphinemaindex and Pierceûs Disease,” American Journal of Enology and Viticulture, Vol. 58, 2007, pp. 494-498.
[10] N. Manigbas and L. Villegas, “Microsatellite Markers in Hybridity Tests to Identify True Hybrids of Sugarcane,” Philippine Journal of Crop Science, Vol. 29, 2004, pp. 23-32.
[11] S. Gomez, N. Denwar, T. Ramasubramanian, C. Simpson, G. Burow, J. Burke, N. Puppala and M. Burow, “Identification of Peanut Hybrids Using Microsatellite Markers and Horizontal Polyacrylamide Gel Electrophoresi,” Peanut Science, Vol. 35, No. 2, 2008, pp. 123-129.
[12] J.-F. Li, G.-B. Ma and L. Xu, “SSR Markers for Identification of Purity of Melon Hybrids,” Chinese Journal of Agricultural Biotechnology, Vol. 5, No. 3, 2008, pp. 223-229.
[13] C. Mohan, P. Shanmugasundaram and N. Senthil, “Identification of True Hybrid Progenies in Cassava Using Simple Sequence Repeat (SSR) Markers,” Bangladesh Journal of Botany, Vol. 42, No. 1, 2013, pp. 155-159.
[14] G. Otti, F. Ayodele, A. Ikpan and G. Melaku, “Development of Genomic Tools for Verification of Hybrids and Selfed Progenies in Cassava (Manihot Esculenta),” African Journal of Biotechnology, Vol. 10, 2011, pp. 17400-17408.
[15] G. Vinay, D. Grant, E. Alan, J. Philip and G. Bryan, “Gel versus Capillary Electrophoresis Genotyping for Categorizing Treatment Outcomes in Two Anti-Malarial Trials in Uganda,” Malaria Journal, 2010, pp. 9-19.
[16] J. Butler, E. Buel, C. Federica and R. Bruce, “Forensic DNA Typing by Capillary Electrophoresis Using the ABI Prism 310 and 3100 Genetic Analyzers for STR Analysis,” Electrophoresis, Vol. 25, 2004, pp. 1397-1412.
[17] P. Chavarriaga-Aguirre, M. M. Maya, M. W. Bonierbale, S. Kresovich, M. A. Fregene, J. Tohme and G. Kochert, “Microsatellites in Cassava (Manihot esculenta Crantz): Discovery, Inheritance and Variability,” Theoretical and Applied Genetics, Vol. 97, 1998, pp. 493-501.
[18] R. E. C. Mba, P. Stephenson, K. Edwards, S. Melzer, J. Mkumbira, U. Gullberg, K. Apel, M. Gale, J. Tohme and M. Fregene, “Simple Sequence Repeat (SSR) Markers Survey of the Cassava (Manihot esculenta Crantz) Genome: Towards an SSR-Based Molecular Genetic Map of Cassava,” Theoretical and Applied Genetics, Vol. 102, 2001, pp. 21-31.
[19] K. Kawano, “Cassava,” In: W. R. Fehr and H. H. Hadley, Eds., Hybridization of Crop Plants, American Society of Agronomy, Madison, 1980, pp. 225-233.
[20] J. J. Doyle and J. L. Doyle, “A Rapid DNA Isolation Procedure for Small Quantities of Fresh Leaf Tissue,” Phytochemical Bulletin, Vol. 19, 1987, pp. 11-15.
[21] R. Powell and F. Gannon, “Purification of DNA by Phenol Extraction and Ethanol Precipitation,” Oxford Practical Approach Series, Oxford University Press, Oxford, 2002.

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.