Quantitative trait loci associated to agronomic traits and yield components in a Sorghum bicolor L. Moench RIL population cultivated under pre-flowering drought and well-watered conditions


The present study aims to identify QTL influencing agronomic traits and yield components under well-watered and pre-flowering drought stress conditions. One hundred F5 recombinant inbred lines (RIL) and the parental lines of a cross between a drought-tolerant and a susceptible line in a field experiment were carried out at Nong Lam University of Ho Chi Minh City, Vietnam. Drought stress was induced by withholding irrigation water from the plants at four weeks after sowing to flowering. Leaf area of the third leaf, stem diameter, plant height, days to heading, anthesis and maturity, panicle length, number of seeds per plant, hundred kernel weight and grain yield were measured. Plants were genotyped with 117 Diversity Arrays Technology (DArT) and eight expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers. Composite interval mapping was carried out on the traits and significant QTL were claimed at a logarithm of the odds (LOD) score >2.5. A total of 50 QTL were detected on nine chromosomes or 13 linkage groups, respectively. Six promising QTL regions with seven QTL for yield and agronomic traits especially related to pre-flowering drought tolerance were identified on chromosomes SBI-01, SBI-03, SBI-04, SBI-05 and SBI-07.

Share and Cite:

Phuong, N. , Stützel, H. and Uptmoor, R. (2013) Quantitative trait loci associated to agronomic traits and yield components in a Sorghum bicolor L. Moench RIL population cultivated under pre-flowering drought and well-watered conditions. Agricultural Sciences, 4, 781-791. doi: 10.4236/as.2013.412107.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Blum, A. (1988) Plant breeding for stress environments. CRC Press, Boca Raton.
[2] Sanchez, A.C., Subudhi, P.K., Rosenow, D.T. and Nguyen, H.T. (2002) Mapping QTLs associated with drought resistance in sorghum (Sorghum bicolor L. Moench). Plant Molecular of Biology, 48, 713-726.
[3] Kebede, H., Subudhi, P.K., Rosenow, D.T. and Nguyen, H.T. (2001) Quantitative trait loci influencing drought tolerance in grain sorghum (Sorghum bicolor L. Moench). Theoretical and Applied Genetics, 103, 266-276.
[4] Tuinstra, M.R., Grote, E.M., Goldsbrough, P.B. and Ejeta, G. (1996) Identification of quantitative trait loci associated with pre-flowering drought tolerance in sorghum. Crop Science, 36, 1337-1344.
[5] Nooden, L.D., Guiamet, J.J. and John, I. (1997) Senescence mechanisms. Physiologia Plantarum, 101, 746-753.
[6] Xu, W., Subudhi, P.K., Crasta, O.R., Rosenow, D.T., Mullet, J.E. and Nguyen, H.T. (2000) Molecular mapping of QTLs conferring stay-green in grain sorghum (Sorghum bicolor L. Moench). Genome, 43, 461-469.
[7] Rosenow, D.T. and Clark, L.E. (1981) Drought tolerance in sorghum. Proceeding of 36th Annual Corn and Sorghum Research Conference, Chicago, 9-11 December 1981, pp. 18-31.
[8] Sambatti, J.B.M. and Caylor, K.K. (2007) When is breeding for drought tolerance optimal if drought is random? New Phytologist, 175, 70-80.
[9] Bohnert, H.J., Nelson, D.E. and Jensen, R.G. (1995) Adaptation to environmental stresses. Plant Cell, 7, 1099-1111.
[10] Tuinstra, M.R., Ejeta, G. and Goldsbrough, P.B. (1997) Heterogenous in bred family (HIF) analysis: An approach for developing near-isogenic lines that differ at quantitative trait loci. Theoretical and Applied Genetics, 95, 1005-1011http://dx.doi.org/10.1007/s001220050654
[11] Crasta, O.R., Xu, W., Rosenow, D.T., Mullet, J.E. and Nguyen, H.T. (1999) Mapping of post-flowering drought resistance traits in grain sorghum: Association of QTLs influencing premature senescence and maturity. Molecular General Genetics, 262, 579-588.
[12] Tao, Y.Z., Henzell, R.G., Jordan, D.R., Butler, D.G., Kelly, A.M. and Mcintyre, C.L. (2000) Identification of genomic region associated with stay-green in sorghum by testing RILs in multiple environments. Theoretical and Applied Genetics, 100, 1225-1232.
[13] Haussmann, B.I.G., Hess, D.E., Seetharama, Welz, H.G. and Geiger, H.H. (2002) Construction of a combined sorghum linkage map from two recombinant inbred populations using AFLP, SSR, RFLP and RAPD markers and comparison with other sorghum maps. Theoretical and Applied Genetics, 105, 629-637.
[14] Harris, K., Subudhi, P.K., Borrell, A., Jordan, D., Rosenow, D., Nguyen, H., Klein, P. and Mullet, J. (2007) Sorghum stay-green QTL individually reduce post-flowering drought-induced leaf senescence. Journal of Experimental Botany, 58, 327-338.
[15] Srinivas, G., Satish, K., Mohali, S.M., Reddy, R.N., Madhusudhana, R., Balakrishna, D., Venkatesh, B.B., Howarth, C.J. and Seetharama, N. (2008) Development of genic-microsatellite markers for sorghum staygreen QTL using comparative genomic approach with rice. Theoretical and Applied Genetics, 117, 703-717.
[16] Srinivas, G., Satish, K., Madhusudhana, R., Reddy, R.N., Mohan, S.M. and Seetharama, N. (2009) Identification of quantitative trait loci for agronomically important traits and their association with genic-microsatellites markers in sorghum. Theoretical and Applied Genetics, 118, 1439-1454. http://dx.doi.org/10.1007/s00122-009-0993-6
[17] Jaccoud, D., Peng, K., Feinstein, D. and Kilian, A. (2001) Diversity arrays: A solid state technology for sequence information independent genotyping. Nucleic Acids Research, 29, e25. http://dx.doi.org/10.1093/nar/29.4.e25
[18] Wenzl, P., Carling, J., Kudrna, D., Jaccoud, D., Huttner, E., Kleinhofs, A. and Kilian, A. (2004) Diversity Arrays Technology (DArT) for whole-genome profiling of barley. PNAS, 101, 9915-9920.
[19] Mace, E.S., Xia, L., Jordan, D.R., Halloran, K., Parh, D.K., Huttner, E., Wenzl, P. and Kilian, A. (2008) DArT markers: Diversity analyses and mapping in Sorghum bicolor. BMC Genomics, 9, 26.
[20] SAS Institute Inc. (2008) User’s guide, version 9.2. SAS Institute Inc., Cary.
[21] Smalley, M.D., Daub, J.L. and Hallauer, A.R. (2004) Estimation of heritability in maize by parent-offspring regression. Maydica, 49, 221-229.
[22] Doyle, J.J. and Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin, 19, 11-15
[23] van Ooijen, J.W. (2006) JoinMap 4® Software for the calculation of genetic linkage maps in experimental populations. Wageningen, The Netherland, 56 p.
[24] Utz, H.F. and Melchinger, A.E. (1996) PLABQTL: A program for composite interval mapping of QTL. Journal of Quantitative Trait Loci, 2, 1-5.
[25] Jansen, R.C. and Stam, P. (1994) High resolution of quantitative traits into multiple loci via interval mapping. Genetics, 136, 1447-1455.
[26] Harley, C.S. and Knott, S.A. (1992) A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity, 69, 315-324.
[27] Ejeta, G., Goldsborough, M.R., Tunistra, E.M., Grote, A., Menkir, Y., et al. (2000) Molecular marker application in sorghum. In: Hausmann, B.I.G., et al., Eds., Application of Molecular Markers in Plant Breeding, Training Manual for a Seminar Held at IITA, Ibadan, 16-17 August 1999, 81-89.
[28] Rosenow, D.T., Ejeta, G., Clark, L.E., Gilbert, M.L., Henzell, R.G., Borell, A.K. and Muchow, R.C. (1996) Breeding for pre- and post flowering drought stress resistance in sorghum. Proceedings of International Conference on Genetic Improvement of Sorghum and Pearl Millet, Lubbock, 23-27 September, 400-411.
[29] Mace, E.S., Rami, J., Bouchet, S., Klein, P.E., Klein, R.R., Kilian, A., Wenzl, P., Xia, L., Halloran, K. and Jordan, D.R. (2009) A consensus genetic map of sorghum that integrates multiple component maps and high throughput Diversity Array Technology (DarT) markers. BMC Plant Biology, 9, 13. http://dx.doi.org/10.1186/1471-2229-9-13
[30] Remington, D.L. and Purugganan, M.D. (2003) Candidate gene, quantitative loci, and functional trait evolution in plants. International Journal of Plant Science, 164, S7-S20. http://dx.doi.org/10.1086/367812
[31] Uptmoor, R., Wenzel, W.G., Ayisi, K.K., Donaldson, G., Gehringer, A., Friedt, W. and Ordon, F. (2006) Variation of the genomic proportion of the recurrent parent in BC1 and its relation to yield performance in sorghum (Sorghum bicolor) breeding for low input agriculture. Plant Breeding, 125, 532-534.

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.