The Phenotypic Conversion of Macrophage Detected by FACS in EAE Mice Treated with or without Fasudil

Chunyun Liu, Yong Xie, Yanhua Li, Jiezhong Yu, Ling Feng, Shaowei Hou, Haifei Zhang, Cungen Ma, Baoguo Xiao

Institute of Brain Science, Department of Neurology, Medical School, Shanxi Datong University, Datong, China.
Institute of Neurology, Huashan Hospital, Fudan University, Shanghai, China.

DOI: 10.4236/eng.2013.510B101                

Abstract

We studied the changes of macrophage populations in splenic mononuclear cells of experimental autoimmune ence-phalomyelitis (EAE) mice treated with or without Fasudil. Phenotypic analysis using flow cytometry showed that the levels of TLR4, CD11c and CD40 which represent the type 1 macrophage, were depressed in Fasudil-treated mice. Incontrast, it was observed the expressions of CD200 and CD14 which typify the type 2 macrophage were elevated in Fasudil-treated mice as compared to EAE mice. And we also found that Fasudil at dose of 40 mg/kg alleviated the se-verity of symptom in EAE mice. Based on the evidence that M1 macrophages are neurotoxic and M2 macrophages promote a regenerative growth, indicating that polarization and shifting of macrophages into M2 cells may also play key roles in treatment of EAE.

Keywords

Experiment Autoimmune Excephomeylitis; Fasudil; Flow Cytometry; Macrophage

Conflicts of Interest

The authors declare no conflicts of interest.

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