Simple, Inexpensive and Ecologically Friendly Derivative Spectrophotometric Fluconazole Assay from Nail Lacquer Formulations
Alisa Elezović, Amar Elezović, Sabira Hadžović
DOI: 10.4236/ajac.2011.22012   PDF    HTML     4,819 Downloads   9,458 Views   Citations


Nail lacquers represent new drug form specifically designed to treat infected nail plate. They are complex organic solutions with specific assaying problems due to the high content of the polymer and plasticizer. Furthermore, there is a lack of assaying methods of active substances from this type of formulations in scientific literature. We developed derivative UV-spectrophotometric method for determination of fluconazole content in antifungal nail lacquer formulations. The method was validated for specificity, linearity, precision (repeatability), intermediate precision and accuracy (recovery). The method is specific, linear in the range of 99.53 - 497.65 μg/ml, precise and showed good recovery (98.79% - 101.77% from all six developed formulations). Besides, it is inexpensive, simple and nontoxic, i.e. ecologically acceptable. This method can be used for assaying fluconazole from this type of formulations.

Share and Cite:

A. Elezović, A. Elezović and S. Hadžović, "Simple, Inexpensive and Ecologically Friendly Derivative Spectrophotometric Fluconazole Assay from Nail Lacquer Formulations," American Journal of Analytical Chemistry, Vol. 2 No. 2, 2011, pp. 109-115. doi: 10.4236/ajac.2011.22012.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] J. Andre, “The Normal Nail,” In: A. O. Barel, M. Paye and H. I. Maibach, Eds., Handbook of Cosmetic Science and Technology, Marcel Dekker, New York, 2001, pp. 29-34.
[2] J. Obadiah and R. Scher, “Nail Disorders: Unapproved Treatments,” Clinical Dermatology, Vol. 20, No. 6, 2002, pp. 643-648. doi:10.1016/S0738-081X(02)00285-7
[3] P. Rich, R. K. Scher, D. Breneman, R. C. Savin, D. S. Feingold, N. Konnikov, J. L. Shupack, S. Pinnell, N. Levine, N. J. Lowe, R. Aly, R. B. Odom, D. L. Greer, M. R. Morman, A. D. Bucko, E. H. Tschen, B. E. Elewski, E. B. Smith and J. Hilbert, “Pharmacokinetics of Three Doses of Once-Weekly Fluconazole (150, 300, 450 mg) in Distal Subungual Onychomycosis of the Toenail,” Journal of the American Academy Dermatology, Vol. 38, No. 6, 1998, pp. 103-109. doi:10.1016/S0190-9622(98)70493-1
[4] R. C. Savin, L. Drake, D. Babel, D. M. Stewart, P. Rich, M. R. Ling, D. Breneman, R. K. Scher, A. G. Martin, D. M. Pariser, R. J. Pariser, C. N. Ellis, S. Kang, D. Friedman, H. I. Katz, C. J. McDonald, J. Muglia, G. Webster, B. E. Elewski, J. J. Leyden, A. D. Bucko, E. H. Tschen, J. M. Hanifin, M. R. Morman, J. L. Shupack, N. Levine, N. J. Lowe, W. F. Bergfeld, C. Camisa, D. S. Feingold, N. Konnikov, R. B. Odom, R. Aly, D. L. Greer and J. Hilbert, “Pharmacokinetics of Three Once-Weekly Dosages of Fluconazol
[5] G. K. McEvoy, “Pharmacist’s Drug Handbook,” American Society of Health-System Pharmacists Corp., Washington D.C., 2001.
[6] S. Murdan, “Drug Delivery to the Nail Following Topical Application,” International Journal of Pharmaceutics, Vol. 236, No. 1-2, 2002, pp. 1-26. doi:10.1016/S0378-5173(01)00989-9
[7] J. W. Wegener, J. C. Klamer, H. Govers and U. A. Th. Brinkman, “Determination of Organic Colorants in Cosmetic Products by High-Performance Liquid Chromatography,” Chromatographia, Vol. 24, No. 1, 1987, pp. 865-875. doi:10.1007/BF02688601
[8] S. C. Rastogi, V. J. Barwick and S. V. Carter, “Identification of Organic Colourants in Cosmetics by HPLC-Diode Array Detection,” Chromatographia, Vol. 45, No. 1, 1997, pp. 215-228. doi:10.1007/BF02505563
[9] R. T. Rivero and V. Topiwala, “Quantitative Determination of Formaldehyde in Cosmetics Using a Combined Solid-Phase Microextraction-Isotope Dilution Mass Spec- trometry Method,” Journal of Chromatography A, Vol. 1029, No. 1-2, 2004, pp. 217-222. doi:10.1016/j.chroma.2003.12.054
[10] D. De Orsi, L. Gagliardi, R. Porra, S. Berri, P. Chimenti, A. Granese, I. Carpani and D. Tonelli, “A Environmentally Friendly Reversed-Phase Liquid Chromatography Method for Phthalates Determination in Nail Cosmetics,” Analytica Chimica Acta, Vol. 555, No. 2, 2006, pp. 238-241. doi:10.1016/j.aca.2005.09.029
[11] H. Chen, C. Wang, X. Wang, N. Hao and J. Liu, “Determination of Phthalate Esters in Cosmetics by Gas Chromatography with Flame Ionization Detection and Mass Spectrometric Detection,” International Journal of Cosmetic Science, Vol. 27, No. 4, 2005, pp. 205-210. doi:10.1111/j.1467-2494.2005.00249.x
[12] Harmonized Tripartite Guideline, “Validation of Analytical Procedures: Text and Methodology Q2 (R1),” Proceedings of the International Conference on Harmonization, Geneva, November 2005, pp. 1-13.
[13] N. G. Goger and H. Y. Aboul-Enein, “Quantitative Determination of Fluconazole in Capsules and IV Solutions by UV Spectrophotometric Methods,” Analytical Letters, Vol. 34, No. 12, 2001, pp. 2089-2098. doi:10.1081/AL-100106841
[14] H. Y. Aboul-Enein, N. G. Goger and A. Turkalp, “Quantitative Determination of Fluconazole in Syrups by First Order Derivative Spectrophotometry,” Analytical Letters, Vol. 35, No. 7, 2002, pp. 1193-1204. doi:10.1081/AL-120005972

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.