Is there a connection between synthetic bone grafts and sisters chromatide exchange?


Background: In oral and maxillofacial surgery, synthetic bone grafts are most widely used as bone substitutes, due to the limited sources of autologous bone. The aim of this study was to examine the influence of three different synthetic bone grafts (Cerasorb, Fortoss and Perioglass) on sisters chromatide exchanges (SCEs) in peripheral lymphocytes. Materials and Methods: Peripheral blood samples taken from 68 patients (45 females and 23 males), who underwent oral surgery procedures, such as apical resection, cyst enucleation or periodontal curretage, were obtained for SCE a day before and two months after the surgeries. A control group included 30 patients, while the study group was made of the patients who underwent bone grafting with Cerasorb? (11 patients), Fortoss? VITAL (10 patients) or Perioglass? (17 patients). Results: Comparing with the results of the study group before and after the treatment, it was concluded that the results were statistically significant (p = 0.001). In the Perioglass? subgroup, a greater statistical significance (p = 0.003) was noted, than that in either the Cerasorb? (p = 0.620) or Fortoss? (p = 0.210) subgroups, in which there was no statistical significance. Conclusions: Although further investigations may be necessary, our results suggest that the synthetic bone grafts might have an influence on SCE in peripheral lymphocytes.

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Köseoğlu, B. , Brkić, A. , Erdem, M. , Öztürk, Ş. , Palanduz, Ş. and Çefle, K. (2013) Is there a connection between synthetic bone grafts and sisters chromatide exchange?. Open Journal of Stomatology, 3, 447-451. doi: 10.4236/ojst.2013.38074.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Rojbani, H., Nyan, M., Ohya, K. and Kasugai, S. (2011) Evaluation of the osteoconductivity of α-tricalcium phosphate, β-tricalcium phosphate, and hydroxyapatite combined with or without simvastatin in rat calvarial defect. Journal of Biomedical Materials Research Part A, 98A, 488-498.
[2] Brugnami, F., Caiazzo, A. and Leone, C. (2009) Local intraoral autologous bone harvesting for dental implant treatment: Alternative sources and criteria of choice. The Keio Journal of Medicine, 58, 24-28.
[3] Wheeler, D.L., Stokes, K.E., Hoellrich, R.G., Chamberland, D.L. and McLoughlin, S.W. (1998) Effect of bioactive glass particle size on osseous regeneration of cancellous defects. Journal of Biomedical Materials Research, 41, 527-533.<527::AID-JBM3>3.0.CO;2-E
[4] Kaptein, M.L., de Putter, C., de Lange, G.L. and Blijdorp, P.A. (1998) Survival of cylindrical implants in composite grafted maxillary sinuses. Journal of Oral and Maxillofacial Surgery, 56, 1376-1380.
[5] Tadjoedin, E.S., de Lange, G.L., Lyaruu, D.M., Kuiper, L. and Burger, E.H. (2002) High concentrations of bioactive glass material (BioGran) vs. autogenous bone for sinus floor elevation. Clinical Oral Implants Research, 13, 428-436.
[6] Wetzel, A.C., Stich, H. and Caffesse, R.G. (1995) Bone apposition onto oral implants in the sinus area filled with different grafting materials. A histological study in beagle dogs. Clinical Oral Implants Research, 6, 155-163.
[7] Hürzeler, M.B., Kirsch, A., Ackermann, K.L. and Quiñones, CR. (1996) Reconstruction of the severely resorbed maxilla with dental implants in the augmented maxillary sinus: A 5-year clinical investigation. International Journal of Oral & Maxillofacial Implants, 11, 466-475.
[8] Xynos, I.D., Edgar, A.J., Buttery, L.D.K., Hench, L.L. and Polak, M. (2001) Gene expression profiling of human osteoblasts following treatment with the ionic products of BioglassR 45S5 dissolution. Journal of Biomedical Materials Research, 55, 151-157.<151::AID-JBM1001>3.0.CO;2-D
[9] Hench, L.L. and Paschall, H.A. (1973) Direct chemical bond of bioactive glass-ceramic materials to bone and muscle. Journal of Biomedical Materials Research, 7, 25-42.
[10] Hench, L.L., Andersson, O.H. and LaTorre, G.P. (1991) The kinetics of bioactive ceramics part III: Surface reactions for bioactive glasses compared with an inactive glass. Bioceramics, 4, 156-162.
[11] Aichelmann-Reidy, M.E. and Yukna, R.A. (1998) Bone replacement grafts. The bone substitutes. Dental Clinics of North America, 42, 491-503.
[12] Albrektsson, T. and Johansson, C. (2001) Osteoinduction, osteoconduction and osseointegration. European Spine Journal, 10, S96-101.
[13] Schepers, E., Declercq, M., Ducheyne, P. and Kempeneers, R. (1991) Bioactive glass particulate material as a filler for bone lesions. Journal of Oral Rehabilitation, 18, 439-452.
[14] Ducheyne, P. (1999) Effect of bioactive glass particle size on osseous regeneration. Journal of Biomedical Materials Research, 46, 301-304.<301::AID-JBM20>3.0.CO;2-#
[15] Nyan, M., Sato, D., Kihara, H., Machida, T., Ohya, K. and Kasugai, S. (2009) Effects of the combination with alpha-tricalcium phosphate and simvastatin on bone regeneration. Clinical Oral Implants Research, 20, 280-287.
[16] Koseoglu, B.G., Ozturk, S., Kocak, H., Palanduz, S. and Cefle, K. (2008) The effects of etodolac, nimesulid and naproxen sodium on the frequency of sister chromatid exchange after enclused third molars surgery. Yonsei Medical Journal, 49, 742-747.
[17] Oztürk, S., Köseoglu, B.G., Koöak, H., Palanduz, S., Cefle, K. and Erkal, H. (2004) In vitro effects of selective and non-selective nonsteroidal anti-inflammatory drugs on the frequency of sister chromatid exchanges. Drugs in R&D, 5, 327-330.
[18] Carbonell, E., Peris, F., Xamena, N., Creus, A. and Marcos, R. (1995) SCE analysis in human lymphocytes of a Spanish control population. Drugs in R&D, 335, 35-46.
[19] Sahin, A., Tatar, A., Oztas, S., Seven, B., Varoglu, E., Yesilyurt, A. and Ayan, AK. (2009) Evaluation of the genotoxic effects of chronic low-dose ionizing radiation exposure on nuclear medicine workers. Nuclear Medicine and Biology, 36, 575-578.
[20] Istifli, E.S. and Topaktas, M. (2010) Cytogenetic genotoxicity of amoxicillin. Environmental and Molecular Mutagenesis, 51, 222-228.
[21] Xie, X., Zhou, Q., Bao, Q., He, Z. And Bao, Y. (2011) Genotoxicity of tetracycline as an emerging pollutant on root meristem cells of wheat (Triticum aestivum L.). Environmental Toxicology, 26, 417-423.
[22] Jaju, M., Jaju, M. and Ahuja, Y.R. (1984) Evaluation of genotoxicity of ampicillin and carbenicillin on human lymphocytes in vitro: Chromosome aberrations, mitotic index, cell cycle kinetics, satellite associations of acrocentric chromosomes and sister chromatid exchanges. Human Toxicology, 3, 173-191.
[23] Köseoglu, V., Kismet, E., Soysal, Y., Ulucan, H., Dündaröz, R., Imirzalioglu, N. and Gökçay, E. (2004) Investigation of DNA damage in lymphocytes exposed to benzathine penicillin G. Pediatrics International, 46, 415-418.
[24] Dündaröz, R., Ozisik, T., Baltaci, V., Karapinar, K., Aydin, H.I. and Denli, M. (2001) Sister-chromatid exchange analysis on long-term benzathine penicillin for secondary prophylaxis of rheumatic fever. Indian Journal of Pediatrics, 68, 121-122.
[25] Ozkul, Y., Erenmemisoglu, A., Ekecik, A., Saatci, C., Ozdamar, S. and Demirtas, H. (1996) Do non-steroidal anti-inflammatory drugs induce sister chromatid exchanges in T lymphocytes? Journal of International Medical Research, 24, 84-87.
[26] Kamitakahara, M., Ohtsuki, C. and Miyazaki, T. (2008) Review paper: Behavior of ceramic biomaterials derived from tricalcium phosphate in physiological condition. Journal of Biomaterials Applications, 23, 197-212.
[27] Subbaiah, R. and Thomas, B. (2011) Efficacy of a bioactive alloplast, in the treatment of human periodontal osseous defects-a clinical study. Medicina Oral, Patología Oral y Cirugía Bucal, 16, e239-244.

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