Relationships between a Thiol-Disulfide System and Liposoluble Antioxidants with Cytogenetic Indices in Humans Exposed to Low Doses Radiation


This work presents the results of biochemical examination of people exposed to irradiation as a result of the Chernobyl catastrophe. In remote period ( in 4, 5, 6 and 7 years) after Chernobyl accident we studied the state of the redox system of glutathione(GSH, GSSG) and the response of the system of essential lipid antioxidants (vitamin E, A) in blood plasma of people of various ages. An analysis of correlations between cytogenetic indices in lymphocytes and levels of reduced glutathione and liposoluble antioxidants in the plasma of peripheral blood in children born after the Chernobyl accident and liquidators is presented. The cumulative doses for the examined group of children received by their mothers from 0.8 to 70 cSv and liquidators received, on average, the highest irradiation doses from 0.1 to 150 cSv. A complex relationship between lipo-and water-soluble antioxidants level in plasma in human population (children living in radionuclide-contaminated regions and the Chernobyl liquidators) exposed to chronic low-level radiation after the Chernobyl accident was demonstrated. The obtained experimental data indicate different responses of the human population water-and fat-soluble antioxidants system to low (from 0.1 to 20 cSv) and high (from 20 to 150 cSv) doses of ionizing radiation.

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Ivanenko, G. and Burlakova, E. (2013) Relationships between a Thiol-Disulfide System and Liposoluble Antioxidants with Cytogenetic Indices in Humans Exposed to Low Doses Radiation. Engineering, 5, 62-67. doi: 10.4236/eng.2013.510B013.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] W. F. Morgan and J. L. Schwartz, “Environmental Mutagen Society Symposium on Risks of Low Dose, Low Dose Rate Exposures of Ionizing Radiation to Humans,” International Journal of Radiation Biology, Vol. 83, 2007, pp. 491-499.
[2] A. V. Akleev “Tissue Reaction under Chronic Exposure to Ionizing Radiation,” Radiation Biology. Radioecology (Russian), Vol. 49, No. 1, 2009, pp. 5-20.
[3] N. E. A. Crompton, “Programmed Cell Response to Ionizing Radiation Damage,” Acta Oncologica, Vol. 37, 1998, pp. 129-142.
[4] R. D. Govorun, “Cytogenetic Damage and Mutagenesis in Mammalian and Human Cells Induced by Ionizing Radiation with Varying LET,” Radiation Biology. Radio-ecology (Russian), Vol. 37, No. 4, 1997, pp. 539-548.
[5] J. Glavind, “Antioxidants in Animal Tissue,” Acta Chemica Scandinavica, Vol. 17, 1963, pp. 1635-1640.
[6] V. V. Sokolovskii, “A Thiol-Disulfide System in n Organism Reaction to Environmental Factors,” Nauka, Sankt-Peterburg, 2008, p. 111.
[7] E. B. Burlakova A. V. Alesenko, E. M. Molochkina, N. P. Palmina and N. G. Khrapova, “Biological Antioxidants in Radiation Dam-age and Malignant Growth,” Nauka, Moscow, 1975, p. 211.
[8] G. F. Ivanenko, “The Role of Antioxidant Activity of Lipids and Endogenous Thiols in Radio Resistance of the Organism,” Extended Abstract of Cand. Set (Biol.) Dissertation, Moscow, 1985, p. 168.
[9] D. D. M. Wayner, G. W. Burton, K. U. Ingold, L. R. C. Barclay and S. J. Locke “The Relative Contributions of Vitamin E, Urate, Ascorbate and Proteins to the Peroxyl Radical-Trapping Antioxidant Activity of Human Blood Plasma,” Biochemica et Biophysica Acta, Vol. 924, 1987, pp. 408-419.
[10] F. B. Pruijn, G. R. M. M. Haenen and A. Bast “Integray between Vitamin E, Glutathione and Dihydrolipoic Acid in Protection against Lipid Peroxidation,” Fat Science Technology, Vol. 93, No. 6, 1991, pp. 216-221.
[11] G. L. Ellman and H. Lysko, “Disulfide and Sulfhydryl Compounds in TCA Extracts of Human Blood and Plasma,” Journal of Laboratory and Clinical Medicine, Vol. 70, 1967, pp. 518-527.
[12] T. L. Mc Neil and L. Y. Beck, “Fluorometric Estimation of GSH-OPT,” Analytical Biochemistry, Vol. 22, 1968, pp. 431-441.
[13] L. G. Hansen and W. G. Warwick “A Fluorometric Micromethod for Serum Vitamins A and E,” Technical Bulletin of the Registry of Medical Technologists, Vol. 39, No. 3, 1969, pp. 70-73.
[14] M. A. Bender, A. A. Awa, A. L. Brooks, P. G. Groer, L. Littlefield, C. Pereira, R. J. Preston and B. W. Wachholz, “Current Status of Cytogenetic Procedures to Detect and Quantify Previous Exposures to Radiation,” Mutation Research, Vol. 196, 1988, pp. 103-159.
[15] L. S. Vartyanyan, S. M. Gurevich, A. I. Kozachenko, L. G. Nagler, E. L. Lozovskaya and E. B. Burlakova, “Changes in Superoxide Production Rate and in Superoxide Dismutase and Glutathione Peroxidase Activities in Subcellular Organelles in Mouse Liver under Exposure to Low Doses of Low-Intensity Radiation,” Biokhimiya (Russian), Vol. 65, No. 4, 2000, pp. 522-527.
[16] L. Hagmar, A. Brogger, I. L. Hansteen, S. Heim, B. Hogstedt, K. Linnainma, F. Mitelman, I. Nordenson, et al., “Cancer Risk in Humans Predicted by Increased Levels of Chromosomal Aberration in Lymphocytes: Nordic Study Group on the Health Risk of Chromosome Damage,” Cancer Research, Vol. 54, 1994, pp. 2919-2922.

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