Micro-environmentally Restricted Yeast Cell Growth within Ca-alginate Microbeads

Ivana Pajic-Lijakovic, Branko Bugarski, Branko Bugarski, Milenko Plavsic, Steva Levic, Ana Kalusevic, Viktor Nedovic

Dept. of Chem. and Polymer Eng., University of Belgrade, Faculty of Technology and Metallurgy, Belgrade, Serbia.
Dept. of Food Technol., University of Belgrade, Faculty of Agriculture, Belgrade, Serbia.

DOI: 10.4236/eng.2012.410B047                

Abstract

Micro-environmental restriction effects to yeast cell growth obtained within Ca-alginate microbeads are considered. It is complex phenomenon influenced by: (1) relaxation of expanded polymer network around the cellular clusters, (2) forces generated by cell growth inside the beads and (3) interactions between solvent, network parts and cells. The resulting effects are measured experimentally by estimating volume of microbeads and yeast cell concentration as function of time of cultivation. Comparative analysis of dynamics of cell growth and increase of microbead volume through four regimes indicates that reversible and irreversible local structural changes of Ca-alginate hydrogel induces micro-environmental restrictions to cell growth. The mechanism of restrictions includes both mechanical and electrostatic effects.

Keywords

Yeast Cells; Micro-environmental Restrictions; Ca-alginate Hydrogel Matrix; Structural Changes; Pattern of Volumetric Yeast Growth

Conflicts of Interest

The authors declare no conflicts of interest.

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