Conditional Replication Adenovirus Sensitizes A549 Cancer Cell to Cisplatin ()
Abstract
Objective: The aim of the study was to
improve the therapeutic effect for lung cancer using a synergetic strategy of
adenovirus-based gene therapy combined with chemotherapy. Methods: A conditional replication adenovirus(CRAd) was employed
to treat the A549 lung cancer cells and cisplatin-resisted A549(A549-DDP) cells.
In vitro MTS / PMS assay were used to evaluate
the cell viability. PCR were used to detect expression of Coxsackie receptor (CAR)
and Multidrug resistance(MDR) gene. The in
vivo anti-tumor effect of CRAd and cisplatin was evaluated using a
subcutaneous mouse model. Results: The CRAd sensitizes A549 cancer cells to
cisplatin. The mechanism of enhanced cell growth inhibition is associated with the
increased CAR and MDR expression. Conclusion: Our approach is better than the
conventional gene therapy and chemotherapy strategy.
Share and Cite:
Y. Liu and Y. Huang, "Conditional Replication Adenovirus Sensitizes A549 Cancer Cell to Cisplatin,"
Engineering, Vol. 4 No. 10B, 2012, pp. 165-166. doi:
10.4236/eng.2012.410B043.
Conflicts of Interest
The authors declare no conflicts of interest.
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