Inheritance of Resistance to Mungbean Yellow Mosaic Virus (MYMV) in Inter and Intra Specific Crosses of Mungbean (Vigna radiata)

M. Sudha, A. Karthikeyan, P. Anusuya, N. M. Ganesh, M. Pandiyan, N. Senthil, M. Raveendran, P. Nagarajan, K. Angappan

Department of Plant Molecular Biology & Biotechnology, Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India;.
National Pulse Research Centre, Tamil Nadu Agricultural University, Vamban, Tamil Nadu, India..

DOI: 10.4236/ajps.2013.410236                

Abstract

The objective of this research was to study the mode of inheritance of resistance to mungbean yellow mosaic virus (MYMV) in inter TNAU RED × VRM (Gg) 1 and intra KMG 189 × VBN (Gg) 2 specific crosses of mungbean. An infector row technique was used for evaluating parents, F₁, F₂ and F₃ plants for MYMV resistance. No insecticide was sprayed in order to maintain the natural whitefly population in experimental field. In the field condition, only after 80% of plants showed MYMV incidence, and the scoring of the test materials was done by MYMV disease rating scale. According to the mean disease score, the mungbean genotypes were categorized into five groups resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible (S) and highly susceptible (HS). 3 (Susceptible): 1 (Resistance) was observed in all two crosses of all F₂ population and it showed that the dominance of susceptibility over the resistance and the results of the F₃ segregation (1:2:1) confirm the segregation pattern of the F₂ segregation. Collectively all the two crosses F₂ and F₃ generations results suggested that a single recessive gene is involved in resistance against the MYMV disease.

Keywords

Inheritance; Mungbean Yellow Mosaic Virus; Vigna spp.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	B. B. Singh, “Project Coordinators Report. Indian Council of Agricultural Research. Indian Institute of Pulses Research. All India Coordinated Research Project on MULLaRP,” Annual Group Meet, 11-13 May 2011.
[2]	Q. M. Haq, A. Arif and V. G. Malathi, “Engineering Resistance against Mungbean Yellow Mosaic India Virus Using Antisense RNA,” Indian Journal of Virology, Vol. 21, No. 1, 2010, pp. 82-85. http://dx.doi.org/10.1007/s13337-010-0003-2
[3]	V. G. Malathi and P. John, “Mungbean Yellow Mosaic Viruses, Desk Encyclopedia of Plant and Fungal Virology in Encyclopedia of Virology,” Elsevier Ltd., Amsterdam, 2008, pp. 364-371.
[4]	D. Singh and P. N. Patel, “Studies on Resistance in Crops to Bacterial Diseases in India, Part VIII. Investigations on Inheritance of Reactions to Bacterial Leaf Spot and Yellow Mosaic Diseases and Linkage, If Any, with Other Characters in Mungbean,” Indian Phytopathology, Vol. 30, 1977, pp. 202-206.
[5]	R. P. Thakur, P. N. Patel and J. P. Verma, “Genetical Relationship between Reaction to Bacterial Leaf Spot, Yellow Mosaic and Cercospora Leaf Spot Diseases in Mungbean (Vigna radiata L.Wilczek),” Euphytica, Vol. 26, No. 3, 1977, pp. 765-774. http://dx.doi.org/10.1007/BF00021705
[6]	M. Saleem, W. A. A. Haris and A. Malik, “Inheritance of Yellow Mosaic Virus in Mungbean (Vigna radiata L. Wilczek),” Pakistan Journal of Phytopathology, Vol. 10, No. 1, 1998, pp. 30-32.
[7]	I. A. Malik, G. Sarwar and Y. Ali, “Inheritance of Tolerance to Mungbean Yellow Mosaic Virus (MYMV) and Some Morphological Characters,” Pakistan Journal of Botany, Vol. 18, No. 1, 1986, pp. 189-198.
[8]	K. R. Reddy and D. P. Singh, “Inheritance of Resistance to Mungbean Yellow Mosaic Virus,” The Madras Agricultural Journal, Vol. 88, No. 2, 1995, pp. 199-201.
[9]	K. S. Reddy, “A New Mutant for Yellow Mosaic Virus Resistance in Mungbean (Vigna radiata L. Wilczek) Variety SML-668 by Recurrent Gamma-Ray Irradiation,” In: Q. Y. Shu, Ed., Induced Plant Mutation in the Genomics Era, Food and Agriculture Organization of the United Nations, Rome, pp. 361-362.
[10]	T. S. Sandhu, J. S. Brar, S. S. Sandhu and M. M. Verma, “Inheritance of Resistance to Mungbean Yellow Mosaic Virus in Greengram,” Journal of Research Punjab Agricultural University, Vol. 22, No. 1, 1985, pp. 607-611.
[11]	R. P. S. Verma and D. P. Singh, “Inheritance of Resistance to Mungbean Yellow Mosaic Virus in Greengram,” Annals of Agricultural Research, Vol. 9, No. 3, 1988, pp. 98-100.
[12]	S. S. Pal, H. S. Dhaliwal and S. S. Bains, “Inheritance of Resistance to Yellow Mosaic Virus in Some Vigna spp.,” Plant Breeding, Vol. 106, No. 2, 1991, pp. 168-171. http://dx.doi.org/10.1111/j.1439-0523.1991.tb00496.x
[13]	S. Ammavasai, D. S. Phogat and I. S. Solanki, “Inheritance of Resistance to Mungbean Yellow Mosaic Virus (MYMV) in Greengram (Vigna radiata L. Wilczek),” The Indian Journal of Genetics, Vol. 64, No. 2, 2004, p. 146.
[14]	G. P. Shukla and B. P. Pandya, “Resistance to Yellow Mosaic in Greengram,” SABRAO Journal, Vol. 17, No. 3, 1985, pp. 165-171.
[15]	K. P. Monika, Singh and P. K. Sareen, “Cytogenetics Studies in Mungbean-Rice Bean Hybrids,” The Journal of Cytology and Genetics, Vol. 2, No. 2, 2001, pp. 13-16.
[16]	M. Pandiyan, N. Ramamoorthi, S. K. Ganesh, S. Jebaraj, P. Nagarajan and P. Balasubramanian, “Broadening the Genetic Base and Introgression of MYMV Resistance and Yield Improvement through Unexplored Genes from Wild Relatives in Mungbean,” Plant Mutation Reports, Vol. 2, No. 2, 2008, pp. 33-43.
[17]	G. Singh, S. Kapoor and K. Singh, “Multiple Disease Resistance in Mungbean with Special Emphasi on Mungbean Yellow Mosaic Virus,” In: S. Shanmugasundaram, Ed., Mungbean Proceedings of the Second International Symbosium on Mungbean Shanhua, Asian Vegetable Research and Development Centre, Tainan, pp. 290-296.