In vitro and greenhouse evaluation for resistance to early blight of potato isolated from Alternaria alternata


Early blight of potato is caused by the fungus Alternaria alternata, one of the most destructive foliar diseases, especially in hot climates under irrigation. In this study, the virus free potato seedlings were obtained from the National Plant Gene Bank of Iran and were inoculated in vitro with a culture filtrate of A. alternate. The leaflets received a 1000-μl droplet of the A. alternata culture filtrate and were inoculated by spraying with a suspension of 105 conidia/ml of isolate A. alternata in the greenhouse method. The experimental design was a completely randomized design (CRD) with three replications and seven genotypes, which have been infected with the two leaves of each iteration. In vitro selection of fungal isolates of A. alternata, chlorotic and necrotic symptoms began 1 to 2 days after inoculation, but the assessment of greenhouse symptoms appeared 6-10 days after inoculation. The area under the disease progress curve values were presented by analysis of variance (ANOVA), and they were compared using Duncan’s test (a = 0.01%). In both methods, there was a significant difference between the potato genotypes (P < 0.01). For In vitro selection and evaluation greenhouse, Casmos were resistant to at least figure and Marfona genotype had the highest resistance.

Share and Cite:

Mirkarimi, H. , Abasi-moghadam, A. and Mozafari, J. (2013) In vitro and greenhouse evaluation for resistance to early blight of potato isolated from Alternaria alternata. Agricultural Sciences, 4, 473-476. doi: 10.4236/as.2013.49063.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Akhtar, P.K., Saleem M.Y., Asghar, M. and Haq, M.A. (2004) New report of Alternaria alternate causing leaf blight oftomato in Pakistan. Plant Pathology, 53, 816. doi:10.1111/j.1365-3059.2004.01099.x
[2] Cassells, A.C. and Kowalski, B. (1998) Strategies for the evaluation of variation as a source of resistance to early and late blight of potato. In: Khurana, P., Chandra, R. and Mahesh, D., Eds., Comprehensive Potato Biotechnology, Malhotra Publishing House, New Delhi, 50-60.
[3] Christ, B.J. and Haynes, K.G.V. (2001) Inheritance to early blight disease in a diploid potato population. Plant Breeding, 120, 169-172. doi:10.1046/j.1439-0523.2001.00565.x
[4] Dita Rodriguez, M.A., Brommonschenkel, S.H., Matsuoka, K. and Mizubuti, E.S.G. (2006) Components of resistance to early blight in four potato cultivars: Effect of leaf position. Journal of Phytopathology, 154, 230-235. doi:10.1111/j.1439-0434.2006.01089.x
[5] Herriott, A.B., Haynes, J.F.L. and Shoemaker, P.B. (1990) Inheritance of resistance to early blight disease in tetraploid x diploid crosses of potatoes. Hortscience, 25, 224-226.
[6] Barksdale, T.H. (1969) Resistance of tomato seedlings to early blight. Phytopathology, 59, 443-446.
[7] Rodriguez, N.V., Kowalski, B., Rodriguez, L.G., Caraballoso, B., Suarez, M.A., Perez, P.O., Quintana, C.R., Gonzalez, N. and Ramos, R.Q. (2007) In vitro and ex vitro selection of potato plantlets for resistance to early blight. Journal of Phytopathology, 155, 582-586. doi:10.1111/j.1439-0434.2007.01282.x
[8] Shaner, G. and Finney, R.E. (1977) The effect of nitrogen fertilization in the expression of slow-mildewing resistance in Knox wheat. Phytopathology, 67, 1051-1056. doi:10.1094/Phyto-67-1051
[9] Pryor, B.M. and Michailides, T.J. (2002) Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with Alternaria late blight of pistachio. Phytopathology, 92, 406-416. doi:10.1094/PHYTO.2002.92.4.406
[10] Martinez, P.R. and Sinclair, M. (1994) Selection in vitro of resistance to Early Blight (Alternaria solani Sorauer.) in Creole Potatoes (Solanum phureja Junz). Fitopatol Colomb, 18, 90-100.
[11] Veitia, N., Dita, M.A., Garcia, L., Herrera, L., Bermu dez, I., Acosta, M., Clavero, J., Orellana, P., Romero, C. and Garcia, L. (2001) Use of tissue culture and the mutagenesis in vitro for the improvement of the resistance to Alternaria solani (Solanum tuberosum Lin.), to var Desiree. Biotecnología Vegetal, 1, 43-47.
[12] Rotem, J. and Feldman, S. (1965) The relation between the ratio of yield to foliage and the incidence of early blight in potato and tomato. Israeli Journal of Agricultural Research, 15, 115-122.
[13] Thompson, P.G.. and Mendoza, H.A. (1984) Genetic variance estimates in a heterogenous potato population propagated from true seed. American Potato Journal, 61, 697-702. doi:10.1007/BF02852933

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.