Modeling of Biomass Production of Haematococcus pluvialis


Microalgae cultivation is justified by the production of high-value fine chemicals and biofuels, essential to reduce the emissions of gases that cause global warming. This paper presents a study of the growth of microalgae Haematococcus pluvialis considering light conditions from 2000 to 10,000 lux, temperature 22?C and pH in the 6.5-12.5 range. The experiments were performed in 4 liter flat plate photobioreactors using the Rudic culture medium. The biomass growth was measured by counting cells in a Neubauer chamber. Both the light intensity and the pH of the medium influenced the rate of growth of the microalgae. A model with exponential behavior was proposed to describe the production of microalgae biomass over time. A nonlinear autoregressive model based on an Artificial Neural Network was used to predict the dynamic behavior of the pH during the growth of the microalgae at different light intensities. Simulations were carried out to analyze the behavior of biomass production at other light intensities within the range considered.

Share and Cite:

R. Galvão, T. Santana, C. Fontes and E. Sales, "Modeling of Biomass Production of Haematococcus pluvialis," Applied Mathematics, Vol. 4 No. 8A, 2013, pp. 50-56. doi: 10.4236/am.2013.48A008.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Y. S. Yun and J. M. Park, “Kinetic Modeling of the Light-Dependent Photosynthetic Activity of the Green Microalga Chlorella vulgaris,” Biotechnology and Bioen gineering, Vol. 83, No. 3, 2003, pp. 303-311. doi:10.1002/bit.10669
[2] S. Amin, “Review on Biofuel Oil and Gas Production Processes from Microalgae,” Energy Conversion and Ma nagement, Vol. 50, No. 7, 2009, pp. 1834-1840. doi:10.1016/j.enconman.2009.03.001
[3] L. Gouveia and A. C.Oliveira, “Microalgae as a Raw Ma terial for Biofuels Production,” Journal of Industrial Mi crobiology & Biotechnology, Vol. 36, No. 2, 2009, pp. 269-274. doi:10.1007/s10295-008-0495-6
[4] P. J. Crutzen, et al., “N2O Release from Agro-Biofuel Production Negates Global Warming Reduction by Re placing Fossil Fuels,” Atmospheric Chemistry and Phys ics, Vol. 8, No. 2, 2008, pp. 389-395. doi:10.5194/acp-8-389-2008
[5] E. Imamoglu, F. V. Sukan and M. C. Dalay, “Effect of Different Culture Media and Light Intensities on Growth of Haematococcus pluvialis,” International Journal of Natural and Engineering Sciences, Vol. 1, No. 3, 2007, pp. 5-9.
[6] Q. L. Dong and X. M. Zhao, “In Situ Carbon Dioxide Fixation in the Process of Natural Astaxanthin Production by a Mixed Culture of Haematococcus pluvialis and Phaffiarhodozyma,” Catalysis Today, Vol. 98, No. 4, 2004, pp. 537-544. doi:10.1016/j.cattod.2004.09.052
[7] V. Ghiggi, “Estudo do Crescimento e Inducao da Pro ducao do pigmentoastaxantinapor Haematococcus pluvialis, Dissertacao (Mestrado),” Programa de Pós-Gradua caoem Engenharia de Bioprocessos e Biotecnologia, Uni versidade Federal do Paraná, Curitiba, 2007.
[8] K. Issarapayup, S. Powtongsook and P. Pavasant, “Flat Panel Airlift Photobioreactors for Cultivation of Vegeta tive Cells of Microalga Haematococcus pluvialis,” Jour nal of Biotechnology, Vol. 142, No. 3-4, 2009, pp. 227 232. doi:10.1016/j.jbiotec.2009.04.014
[9] V. Rudic and T. Dudnícenco, “Process for Cultivation of Green Alga Haematococcus pluvialis (Flotow),” MD Pat ent Nr. A 2000 0154, 2000.
[10] S. O. Lourenco, “Cultivo de Microalgas Marinhas: Prin cípios e Aplicacoes,” Rima, Sao Carlos, 2006.
[11] G. Sthephanopoulos, “Chemical Process Control,” Pren tice-Hall, Upper Saddle River, 1984.
[12] B. Y. Zhang, et al., “Production of Astaxanthin from Haematococcus in Open Pond by Two-Stage Growth One-Step Process,” Aquaculture, Vol. 295, No. 3-4, 2009, pp. 275-281. doi:10.1016/j.aquaculture.2009.06.043
[13] S. Boussiba and A. Vonshak, “Astaxanthin Accumulation in the Green Algae Haematococcus pluvialis,” Plant Cell Physio, Vol. 32, No. 7, 1991, pp. 1077-1082.
[14] A. R. Dominguez-Bocanegra, T. Ponce-Noyola and J. A. Torres-Munoz, “Astaxanthin Production by Phaffiarho dozyma and Haematococcus pluvialis: A Comparative Stu dy,” Applied Microbiology and Biotechnology, Vol. 75, No. 4, 2007, pp. 783-791. doi:10.1007/s00253-007-0889-9
[15] L. Taiz and E. Zeiger, “Fisiologia Vegetal,” 3rd Edition, Artmed, Porto Alegre, 2004.
[16] Z. I. Khalil, et al., “Effect of pH on Growth and Bio chemical Responses of Dunaliellabardawil and Chlorella ellipsoidea,” World Journal of Microbiology & Biotech nology, Vol. 26, No. 7, 2010, pp. 1225-1231. doi:10.1007/s11274-009-0292-z

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.