Determination of Mitochondrial Functional State in Vital Tissues by Cytobiochemical Analysis of Peripheral Blood Lymphocytes

Galina A. Frelikh, Elena A. Yanovskaya, Natalia Yu. Polomeeva, Maxim S. Timofeev, Vladimir A. Slepichev, Olga S. Bryushinina, Yulia G. Zyuz’kova, Roman V. Gurto, Alexander S. Vasil’ev, Elena V. Semiglazova, Vladimir V. Udut

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Laboratory of Physiology, Molecular and Clinical Pharmacology, Federal Governmental Budgetary Organization “Research Institute of Pharmacology” Siberian Branch of the Russian Academy of Medical Sciences, Tomsk, Russia.

DOI: 10.4236/ojemd.2013.32A002                

Abstract

In the work, we offer a brief report by research results demonstrating correlations between energy status of peripheral blood lymphocytes and functional state of vital tissues mitochondria. Basing on our preliminary data, showing correlations between succinate dehydrogenase (SDH) activity in peripheral blood lymphocytes and speed or duration of pyridine nucleotides reduction in mitochondria of rat brain and liver tissues, we propose an idea of using cytobiochemical method of SDH estimation in peripheral blood lymphocytes for evaluation of tissue mitochondria status. The method could be successfully used in clinical practice for early revealing of energy metabolism disturbances that allows preventing disease development and optimizing the diseases therapy.

Keywords

Mitochondria; Energy Metabolism; Lymphocyte; Succinate Dehydrogenase; Cytobiochemical

Conflicts of Interest

The authors declare no conflicts of interest.

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