Determination of Mitochondrial Functional State in Vital Tissues by Cytobiochemical Analysis of Peripheral Blood Lymphocytes


In the work, we offer a brief report by research results demonstrating correlations between energy status of peripheral blood lymphocytes and functional state of vital tissues mitochondria. Basing on our preliminary data, showing correlations between succinate dehydrogenase (SDH) activity in peripheral blood lymphocytes and speed or duration of pyridine nucleotides reduction in mitochondria of rat brain and liver tissues, we propose an idea of using cytobiochemical method of SDH estimation in peripheral blood lymphocytes for evaluation of tissue mitochondria status. The method could be successfully used in clinical practice for early revealing of energy metabolism disturbances that allows preventing disease development and optimizing the diseases therapy.

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G. Frelikh, E. Yanovskaya, N. Polomeeva, M. Timofeev, V. Slepichev, O. Bryushinina, Y. Zyuz’kova, R. Gurto, A. Vasil’ev, E. Semiglazova and V. Udut, "Determination of Mitochondrial Functional State in Vital Tissues by Cytobiochemical Analysis of Peripheral Blood Lymphocytes," Open Journal of Endocrine and Metabolic Diseases, Vol. 3 No. 2A, 2013, pp. 10-13. doi: 10.4236/ojemd.2013.32A002.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] N. Bratic and A. Trifunovic, “Mitochondrial Energy Metabolism and Ageing,” Biochimica et Biophysica Acta (BBA)-Bioenergetics, Vol. 1797, No 6-7, 2010, pp. 961-967. doi:10.1016/j.bbabio.2010.01.004
[2] D. L. Johannsen and E. Ravussin, “The Role of Mitochondria in Health and Disease,” Current Opinion in Pharmacology, Vol. 9, No. 6, 2009, pp. 780-786. doi.10.1016/j.coph.2009.09.002
[3] L. D. Osellame, T. S. Blacker and M. R. Duchen, “Cellular and Molecular Mechanisms of Mitochondrial Function,” Best Practice & Research Clinical Endocrinology & Metabolism, Mitochondria in Endocrinology, Vol. 26, No 6, 2012, pp. 711-723. doi:10.1016/j.beem.2012.05.003
[4] W. S. Lee and R. J. Sokol, “Liver Disease in Mitochondrial Disorders,” Seminars in Liver Disease, Vol. 27, No. 3, 2007, pp. 259-273. doi:10.1055/s-2007-985071
[5] C. Frezza and E. Gottlieb, “Mitochondria in Cancer: Not Just Innocent Bystanders,” Seminars in Cancer Biology, Vol. 19, No. 1, 2009, pp. 4-11. doi:10.1016/j.semcancer.2008.11.008
[6] M. A. Frohman, “Mitochondria as Integrators of Signal Transduction and Energy Production in Cardiac Physiology and Disease,” Journal of Molecular Medicine, Vol. 88, No. 10, 2010, pp. 967-970. doi:10.1007/s00109-010-0662-x
[7] G. M. Enns, “The Contribution of Mitochondria to Common Disorders,” Molecular Genetics and Metabolism, Vol. 80, No. 1-2, 2003, pp. 11-26. doi:10.1016/j.ymgme.2003.08.009
[8] J. A. Dykens and Y. Will, “The Significance of Mitochondrial Toxicity Testing in Drug Development,” Drug Discovery Today, Vol. 12, No 17-18, 2007, pp. 777-785. doi:10.1016/j.drudis.2007.07.013
[9] F. N. Gellerich, J. A. Mayr, S. Reuter, W. Sperl and S. Zierz, “The Problem of Interlab Variation in Methods for Mitochondrial Disease Diagnosis: Enzymatic Measurement of Respiratory Chain Complexes,” Mitochondrion, Vol. 4, No. 5-6, 2004, pp. 427-439. doi:10.1016/j.mito.2004.07.007
[10] A. Mayevsky, R. Walden, E. Pewzner, A. Deutsch, E. Heldenberg, J. Lavee, S. Tager, E. Kachel, E. Raanani, S. Preisman, V. Glauber and E. Segal, “Mitochondrial Function and Tissue Vitality: Bench-to-Bedside Real-Time Optical Monitoring System,” Journal of Biomedical Optics, Vol. 16, No. 6, 2011, Article ID: 067004. doi:10.1117/1.3585674
[11] R. P. Narcissov, “Application of P-Nitro-Violet-Tetrazo-Lium for the Quantitative Cytochemistry of Dehydrogenases in Human Lymphocytes,” Arkhiv Anatomy, Gistology and Embriology, Vol. 56, 1969, pp. 85-91.
[12] M. N. Kondrashova, M. V. Zakharchenko and N. V. Khunderyakova, “Preservation of the in Vivo State of Mitochondrial Network for ex Vivo Physiological Study of Mitochondria,” International Journal of Biochemistry and Cell Biology, Vol. 41, No. 10, 2009, pp. 2036-2050. doi:10.1016/j.biocel.2009.04.020
[13] M. Jesús Tunón, M. Alvarez, J. M Culebras and J. González-Gallego, “An Overview of Animal Models for Investigating the Pathogenesis and Therapeutic Strategies in Acute Hepatic Failure,” World Journal of Gastroenterology, Vol. 15, No 25, 2009, pp. 3086-3098. doi:10.3748/wjg.15.3086
[14] B. Chance, P. Cohen, F. Jobsis and B. Schoener, “Intracellular Oxidation-Reduction States in Vivo,” Science, Vol. 137, No. 3529, 1962, pp. 499-508. doi:10.1126/science.137.3529.499

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