Biodegradable Chitosan/Multiwalled Carbon Nanotube Composite for Bone Tissue Engineering


In this work the influence of multiwalled carbon nanotubes (MWCNT) on mechanical properties was evaluated also the osteoinduction mechanism in Chitosan/MWCNT composite is reported. The morphology and the mechanical properties of the scaffolds were controlled by varying solvent ratios, quenching temperatures and carbon nanotubes concentration. In vitro cell culture of Sprague-Dawley rat’s osteoblasts was used to evaluate the phenotype expression of cells in the scaffolds. The presence of MWCNT in a chitosan matrix improving its mechanical properties and sustain osteoblast growth and differentiation that represent a potential application of the Chitosan/MWCNT as biomaterial for bone tissue engineering.

Share and Cite:

I. Olivas-Armendariz, S. Martel-Estrada, M. Mendoza-Duarte, F. Jiménez-Vega, P. García-Casillas and C. Martínez-Pérez, "Biodegradable Chitosan/Multiwalled Carbon Nanotube Composite for Bone Tissue Engineering," Journal of Biomaterials and Nanobiotechnology, Vol. 4 No. 2, 2013, pp. 204-211. doi: 10.4236/jbnb.2013.42025.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] J. Venkatesam, Z. Qian, B. Ryu, N. Ashok and S. Kim, “Preparation and Characterization of Chitosan-Carbon Nanotube Scaffolds for Bone Tissue Engineering,” International Journal of Biological Macromolecules, Vol. 50, No. 2, 2012, pp. 393-402. doi:10.1016/j.ijbiomac.2011.12.032
[2] L. P. Pan, X. Pei, R. He, Q. Wan and J. Wang, “Multiwall Carbon Nanotubes/Polycaprolactone Composites for Bone Tissue Engineering Application,” Colloids and Surfaces B: Biointerfaces, Vol. 93, No. 5, 2012, pp. 226-234. doi:10.1016/j.colsurfb.2012.01.011
[3] M. D. Raucci, V. D’Antò and V. Guarino, “Biomineralized Porous Composite Scaffolds Prepared by Chemical Synthesis for Bone Tissue Regeneration,” Acta Biomaterialia, Vol. 6, No. 4, 2010, pp. 4090-4099. doi:10.1016/j.actbio.2010.04.018
[4] M. V. Mattioli-Belmonte, V. Giovanni and W. Yudan, “Tuning Polycaprolactone-Carbon Nanotube Composites for Bone Tissue Engineering Scaffolds,” Materials Science and Engineering C, Vol. 32, No. 2, 2012, pp. 152159. doi:10.1016/j.msec.2011.10.010
[5] X. Lu, T. Qiu, X. Wang and F. Zhang, “Preparation of Foam-Like Carbon Nanotubes/Hydroxyapatite Composite Scaffolds with Superparamagnetic Properties,” Applied Surface Science, Vol. 262, No. 1, 2012, pp. 227-230. doi:10.1016/j.apsusc.2012.06.041
[6] M. N. Elsabee, “Chitosan Based Nanofibers, Review,” Materials Science and Engineering C, Vol. 32, No. 7, 2012, pp. 1711-1726. doi:10.1016/j.msec.2012.05.009
[7] J. C. Yang and C. Hsien, “Preparation and Characterization of Polyvibyl Alcohol/Chitosan Blended Membrane for Alkaline Direct Methanol Fuel Cells,” Journal of Membrane Science, Vol. 419-420, No. 7, 2012, pp. 65-71. doi:10.1016/j.memsci.2012.06.051
[8] S. R. Kanatt, M. S. Rao, S. P. Chawla and A. Sharma, “Active Chitosan-Polyvinyl Alcohol Films with Natural Extracts,” Food Hydrocolloids, Vol. 29, 2012, pp. 290287.
[9] D. B. Baskar, R. Balu and T. S. Kumar, “Mineralization of Pristine Chitosan Film through Biomimetic Process,” International Journal of Biological Macromolecules, Vol. 49, No. 3, 2011, pp. 385-389.
[10] O. D. Yilmaz, D. O. Demirkol, S. Gülcemal, A. Kilinc, S. Timur and B. Cetinkauya, “Chitosan-Ferrocene Film as a Platform for Flow Injection Analysis Applications of Glucose Oxidase and Gluconobacter Oxydans Biosensors,” Colloids and Surfaces B: Biointerfaces, Vol. 100, No. 2, 2012, pp. 62-68. doi:10.1016/j.colsurfb.2012.05.020
[11] M. R. Tuhin, O. T. Mohammad, R. Nazia, M. E. Haque, R. A. Khan, N. C. Dafader, R. Islam and M. Nurnabi, “Modification of Mechanical and Thermal Property of Chitosan-Starch Blend Films,” Radiation Physics and Chemistry, Vol. 81, No. 10, 2012, pp. 1659-1668. doi:10.1016/j.radphyschem.2012.04.015
[12] I. B. Leonor, E. T. Baran, M. Kawashita, R. L. Reis, T. Kokubo and T. Nakamura, “Growth of a Bonelike Apatite on Chitosan Microparticles after a Calcium Silicate Treatment,” Acta Biomaterialia, Vol. 4, No. 5, 2008, pp. 13491359. doi:10.1016/j.actbio.2008.03.003
[13] N. M. Leelaviwat, M. Siriporn, P. Chantamee and F. Kajornsak, “Microwave-Induced Fabrication of Copper Nanoparticle/Carbon Nanotubes Hybrid Material,” Current Applied Physics, Vol. 12, No. 6, 2012, pp. 1575-1579. doi:10.1016/j.cap.2012.05.002
[14] X. L. Li, H. Liu, X. Niu, B. Yu, Y. Fam, Q. Feng, F. Cui and F. Watari, “The Use of Carbon Nanotubes to Induce Osteogenic Differentiation of Human Adiposa-Derived MSCs in Vitro and Ectopic Bone Formation in Vivo,” Biomaterials, Vol. 33, No. 19, 2012, pp. 4818-4827. doi:10.1016/j.biomaterials.2012.03.045
[15] N. Wu, W. Quan and A. Behrouz, “Ejection of DNA Molecules from Carbon Nanotubes,” Carbon, Vol. 50, No. 13, 2012, pp. 4945-4952. doi:10.1016/j.carbon.2012.06.026
[16] I. Olivas-Armendáriz, P. Garcia-Casillas, R. MartinezSanchez, A. Martinez-Villafane and C. A. Martinez-Pere, “Chitosan/MWCNT Composites Prepared by Thermal Induced Phase Separation,” Journal of Alloys and Compounds, Vol. 495, No. 2, 2010, pp. 592-595. doi:10.1016/j.jallcom.2009.10.205
[17] S. A. Martel-Estrada, I. Olivas-Armendáriz, C. A. Martínez-Pérez, T. Hernández, E. I. Acosta-Gómez, J. G. Chacón-Nava, F. Jiménez-Vega and P. E. García-Casillas, “Chitosan/Poly(Dllactide-Co-Glycolide) Scaf-folds for Tissue Engineering,” Journal of Materials Science: Materials in Medicine, Vol. 23, No. 12, 2012, pp. 2893-2901. doi:10.1007/s10856-012-4762-8
[18] X. Ba, M. Rafailovich, Y. Meng, N. Pernodet, S. Wirick, Y. Qin and E. Dimasi, “Complementary Effects of MultiProtein Components on Biomineralization in Vitro,” Journal of Structural Biology, Vol. 170, No. 1, 2010, pp. 8392. doi:10.1016/j.jsb.2009.12.018
[19] R. Wells and D. Discher, “Matrix Elasticity, Cytoskeletal Tension, and TGF-Beta: The Insoluble and Soluble Meet,” Science Signal, Vol. 1, No. 10, 2008, p. 13. doi:10.1126/stke.110pe13
[20] S. Misra, F. Ohashi, S. Valappil, J. Knowles, I. Roy, S. Silva, V. Salih and A. Boccaccini, “Characterization of Carbon Nanotube (MWCNT) Containing P(3HB)/Bioactive Glass Composites for Tissue Engineering Applications,” Acta Biomaterialia, Vol. 6, No. 3, 2010, pp. 735742. doi:10.1016/j.actbio.2009.09.023
[21] L. Bacakova, E. Filova, T. Ruml and V. Svorcik, “Modulation of Cell Adhesion, Proliferation and Differentiation on Materials Designed for Body Implants,” Biotechnology Advances, Vol. 29, No. 6, 2011, pp. 739-767. doi:10.1016/j.biotechadv.2011.06.004
[22] S. Okada, H. Ito, A. Nagai, J. Komotori and H. Imai, “Adhesion of Osteoblast-Like Cells on Nanostructured Hydroxyapatite,” Acta Biomaterialia, Vol. 6, No. 2, 2010, pp. 591-597. doi:10.1016/j.actbio.2009.07.037
[23] M. V. García-Garduño and J. Reyes-Gasga, “La Hidroxiapatita, su Importancia en los Tejidos Mineralizados y su Aplicación Biomédica,” Revista Especializada en Ciencias Químico-Biológicas, Vol. 9, No. 1, 2006, pp. 90-95.
[24] M. T. Collins and M. Boehm, “It ANKH Necessarily So,” Journal of Clinical Endocrinology & Metabolism, Vol. 96, No. 1, 2011, pp. 72-74. doi:10.1210/jc.2010-2614
[25] A. Kierszenbaum, “Histología y Biología Celular,” Elsevier España.
[26] K. L. Elias, R. L. Price and T. J. Webster, “Enhanced Functions of Osteoblasts on Nanometer Diameter Carbon Fibers,” Biomaterials, Vol. 23, No. 5, 2008, pp. 32793287. doi:10.1016/S0142-9612(02)00087-X
[27] A. L. Boskey, B. Christensen, H. Taleb and E. S. Sorensen, “Post-Translational Modification of Osteopontin: Effects on in Vitro Hydroxyapatite Formation and Growth,” Biochemical and Biophysical Research Communications, Vol. 419, No. 2, 2012, pp. 333-338. doi:10.1016/j.bbrc.2012.02.024
[28] A. L. Boskey, L. Spevak, M. Tan, S. B. Doty and W. T. Butler, “Dentin Sialoprotein (DSP) Has Limited Effects on in Vitro Apatite Formation and Growth,” Calcified Tissue International, Vol. 67, No. 6, 2000, pp. 472-478. doi:10.1007/s002230001169

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.