In Vitro Propagation and Conservation of Zeyheriamontana Mart: An Endangered Medicinal Plant


Roots of Zeyheriamontana, a species native to the savanna (Cerrado) region of central Brazil, produce lapachol, a naphthoquinone with anticancer activity. Lapachol is also the precursor of β-lapachone, a novel drug candidate for preventive and adjuvant cancer therapies. The leaves of Z. montana are a renewable source of ursolic acid and oleanoic acid, compounds known for their anticancer, antioxidant and antimicrobial properties. The potential prophylactic use of β-lapachone, as well as the medicinal properties of ursolic acid, highlights the importance of this study on Z.montana’s germplasm conservation. Multiple shoots were induced on Woody Plant media with supplemented 0.1 mg·L-1 of thidiazuron (TDZ). Rooting was promoted on half strength WP (Woody Plant media containing 1.0 mg·L-1 of Indolbutiric acid-IBA). Plantlet acclimatization to ex-vitro condition was done at a 70% success rate using different substrates. It was possible to store Z.montana’s elite germplasm using in vitro cultures of media containing 2% sucrose plus 4% sorbitol for six months without subcultures.

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B. Bertoni, R. Moraes, L. Previdelli, P. Pereira, S. França and A. Pereira, "In Vitro Propagation and Conservation of Zeyheriamontana Mart: An Endangered Medicinal Plant," American Journal of Plant Sciences, Vol. 4 No. 3, 2013, pp. 519-523. doi: 10.4236/ajps.2013.43066.

Conflicts of Interest

The authors declare no conflicts of interest.


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