Development of a Performant Method for Glucocapparin Determination in Boscia senegalensis Lam Ex. Poir.: A Study of the Variability

Momar Talla Gueye, Dogo Seck, Abdoulaye Diallo, Danny Trisman, Christophe Fischer, Jean-Paul Barthelemy, Jean-Paul Wathelet, Georges Lognay

Gembloux Agro-Bio Tech, Unit Analysis, Quality, Risks—Laboratory of Analytical Chemistry, University of Liege, Gembloux, Belgium.
Institute of Food Technology (ITA), Hann-Dakar, Senegal.
Regional Centre for Research in Ecotoxicology and Environmental Safety (CERES/Locustox), Dakar, Senegal.
University of Liege, Gembloux Agro-Bio Tech, Unit of General and Organic Chemistry, Gembloux, Belgique.

DOI: 10.4236/ajac.2013.42014                

Abstract

This study describes a glucocapparin determination method. Based on rapeseed determination of glucosinolate (GSL), the equation of the average straight regression line is Y = 100.42X – 0.03 (R² = 0.9998). Enzymatic hydrolysis of glucocapparin extracted from leaves and fruits of B. senegalensis, analyzed by SPME-GC-MS confirmed the presence of methylisothiocyanate as the main hydrolysis glucocapparin product. Monitoring glucocapparin contents in B. senegalensis leaves and fruits collected in 4 localities in Senegal showed differences between organs according localities and periods of harvest. Glucocapparin content was very high in dry season particularly in January and the lowest rates were recorded during the rainy period between August and November.

Keywords

Boscia Senegalensis; Validation Method; Glucocapparin; Monitoring; Senegal

Conflicts of Interest

The authors declare no conflicts of interest.

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