Occurrence of Methicillin-Resistant Staphylococcus aureus in Cheese Produced in German Farm-Dairies


The aim of this study was to determine if raw milk cheese or cheese to be made out of heated milk, manufactured in small German cheese dairies might be a reservoir of contaminations with methicillin-resistant Staphylococcus aureus (MRSA). Due to predominantly manual handling during cheese making, recontaminations with Staphylococcus (S.) aureus or MRSA cannot be excluded. Commercial cheese products (n = 72) available in the region of Hanover(Lower Saxony) and dairy products offered via internet were analyzed with regard to the occurrence of S. aureus and MRSA. Thereof, two cheese samples, manufactured from pasteurized milk and two samples, produced from raw milk, were S. aureus positive with CFU/g between 1.0 × 101 and 7.0 × 101. MRSA was not detected. All analysed cheese samples could be considered safe for consuming.

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C. Zinke, M. Winter, E. Mohr and V. Kromker, "Occurrence of Methicillin-Resistant Staphylococcus aureus in Cheese Produced in German Farm-Dairies," Advances in Microbiology, Vol. 2 No. 4, 2012, pp. 629-633. doi: 10.4236/aim.2012.24082.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] E. Ortega, H. Abriouel, R. Lucas and A. Galvez, “Multiple Roles of Staphylococcus aureus Enterotoxins: Pathogenicity, Superantigenic Activity, and Correlation to Antibiotic Resistance,” Toxins, Vol. 2, 2010, pp. 2117-2131. doi:10.3390/toxins2082117
[2] R. G. M. Olde Riekerink, W. Barkema, S. Veenstra, D. E. Poole, R. T. Dingwell and G. P. Keefe, “Prevalence of Contagious Mastitis Pathogens in Bulk Tank Milk in Prince Edward Island,” Canadian Veterinary Journal, Vol. 47, No. 6, 2006, pp. 567-572.
[3] F. Peles, M. Wagner, L. Varga, I. Hein, P. Rieck, K. Gutser, P. Kereszturi, G. Kardos, I. Turcsanyi, B. Beri and A. Szabo, “Characterization of Staphylococcus aureus Strains Isolated from Bovine Milk in Hungary,” International Journal of Food Microbiology, Vol. 118, No. 2, 2007, pp. 186-193. doi:10.1016/j.ijfoodmicro.2007.07.010
[4] J. E. Virgin, T. M. van Slyke, J. E. Lombard and R. N. Zadoks, “Short Communication: Methicillin-Resistant Staphylococcus aureus Detection in US Bulk Tank Milk,” Journal of Dairy Science, Vol. 92, No. 10, 2009, pp. 4988-4991. doi:10.3168/jds.2009-2290
[5] Y. Le Loir, F. Baron and M. Gautier, “Staphylococcus aureus and Food Poisoning,” Genetics and Molecular Research, Vol. 2, 2003, pp. 63-76.
[6] Commission Regulation (EC) No 1441/2007 of 5 December 2007 Amending Regulation (EC) No 2073/2005 on Microbiological Criteria for Foodstuffs, Official Journal of European Union, L322/12, 2007.
[7] H. J. Jorgensen, T. Mork and L. M. Rorvik, “The Occurrence of Staphylococcus aureus on a Farm with Small-Scale Production of Raw Milk Cheese,” Journal of Dairy Science, Vol. 88, No. 11, 2005, pp. 3810-3817. doi:10.3168/jds.S0022-0302(05)73066-6
[8] ?. Rosengren, A. Fabricius, B. Guss, S. Sylven and R. Lindqvist, “Occurrence of Foodborne Pathogens and Characterization of Staphylococcus aureus in Cheese Produced on Farm-Dairies,” International Journal of Food Microbiology, Vol. 144, 2010, pp. 263-269. doi:10.1016/j.ijfoodmicro.2010.10.004
[9] M. C. D. P. B. Andre, M. R. H. Campos, L. J. Borges, A. Kipnis, F. C. Pimenta and A. B. Serafini, “Comparison of Staphylococcus aureus Isolates from Food Handlers, Raw Bovine Milk and Minas Frescal Cheese by Antibiogram and Pulsed-Field Gel Electrophoresis Following SmaI Digestion,” Food Control, Vol. 19, No. 2, 2008, pp. 200-207. doi:10.1016/j.foodcont.2007.03.010
[10] M. C. Enright, D. A. Robinson, G. Randle, E. Feil, H. Grundmann and B. G. Spratt, “The Evolutionary History of Methicillin-Resistant Staphylococcus aureus (MRSA),” Proceedings of the National Academy of Sciences, Vol. 99, No. 11, 2002, pp. 7687-7692. doi:10.1073/pnas.122108599
[11] B. C. Herold, L. C. Immergluck, M. C. Maranan, D. S. Lauderdale, R. E. Gaskin, S. Boyle-Vavra, C. D. Leitch and R. S. Daum, “Community-Acquired Methicillin-Resistant Staphylococcus aureus in Children,” Japan Automobile Manufacturers Association, Vol. 279, No. 6, 1998, pp. 593-598. doi:10.1001/jama.279.8.593
[12] T. S. Naimi, K. H. LeDell, K. Como-Sabetti, S. M. Borchardt, D. J. Boxrud, J. Etienne, S. K. Johnson, F. Vandenesch, S. Fridkin, C. O’Boyle, R. N. Danila and R. Lynfield, “Comparison of Community and Health Care-Associated Methicillin-Resistant Staphylococcus aureus Infection,” Japan Automobile Manufacturers Association, Vol. 290, No. 22, 2003, pp. 2976-2984. doi:10.1001/jama.290.22.2976
[13] H. F. Chambers, “Methicillin Resistance in Staphylococci: Molecular and Biochemical Basis and Clinical Implications,” Clinical Microbiology Reviews, Vol. 10, No. 4, 1997, pp. 781-791.
[14] A. Voss, F. Loeffen, J. Bakker, C. Klaassen and M. Wulf, “Methicillinresistant Staphylococcus aureus in Pig Farming,” Emerging Infectious Diseases, Vol. 11, No. 12, 2005, pp. 1965-1966. doi:10.3201/eid1112.050428
[15] A. Moodley, E. C. Nightingale, M. Stegger, S. S. Nielsen, R. L. Skov and L. Guardabassi, “High Risk for Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Danish Veterinary Practitioners,” Scandinavian Journal of Work, Environment & Health, Vol. 34, No. 2, 2008, pp. 151-157. doi:10.5271/sjweh.1219
[16] I. van Loo, X. Huijsdens, E. Tiemersma, A. de Neeling, N. van de Sande-Bruinsma, D. Beaujean, A. Voss and J. Kluytmans, “Emergence of Methicillin-Resistant Staphylococcus aureus of Animal Origin in Humans,” Emerging Infectious Diseases, Vol. 13, No. 12, 2007, pp. 1834-1839. doi:10.3201/eid1312.070384
[17] M. Wulf, A. van Nes, A. Eikelenboom-Boskamp, C. Klaassen and A. Voss, “Difference in Carriage of MRSA in Organic and Regular Pig Farmers,” IPVS, Durban, 2008.
[18] M. Nemati, K. Hermans, U. Lipinska, O. Denis, A. Deplano, M. Struelens, L. A. Devriese, F. Pasmans and F. Haesebrouck, “Antimicrobial Resistance of Old and Recent Staphylococcus aureus Isolates from Poultry: First Detection of Livestock-Associated Methicillin-Resistant Strain ST398,” Antimicrobial Agents and Chemotherapy, Vol. 52, No. 11, 2008, pp. 3817-3819. doi:10.1128/AAC.00613-08
[19] Spohr, M., J. Rau, A. Friedrich, G. Klittich, A. Fetsch, B. Guerra, J. A. Hammerl, and B.-A. Tenhagen, “Methicillin-Resistant Staphylococcus aureus (MRSA) in three dairy herds in southwest Germany,” Zoonoses Public Health, Vol. 58, No. 4, 2011, pp. 252-261. doi:10.1111/j.1863-2378.2010.01344.x
[20] J. Vicca, W. Vanderhaeghen, T. Cerpentier and P. Butaye, “Prevalence at Herd-Level of Methicillin Resistant Staphylococcus aureus in Milk Samples of Dairy Herds,” Mastitis Control-From Science to Practice, 2008, pp. 71-75.
[21] E. de Boer, J. T. M. Zwartkruis-Nahuis, B. Wit, X. W. Huijsdens, A. J. de Neeling, T. Bosch, R. A. A. van Oosterom, A. Vila and A. E. Heuvelink, “Prevalence of Methicillin-Resistant Staphylococcus aureus in Meat,” International Journal of Food Microbiology, Vol. 134, No. 1-2, 2009, pp. 52-56. doi:10.1016/j.ijfoodmicro.2008.12.007
[22] A. J. de Neeling, M. J. M. van den Broek, E. C. Spalburg, M. G. van Santen-Verheuvel, W. D. C. Dam-Deisz, H. C. Boshuizen, A .W. van de Giessen, E. van Duijkeren and X. W. Huijsdens, “High Prevalence of Methicillin Resistant Staphylococcus aureus in Pigs,” Veterinary Microbiology, Vol. 122, No. 3-4, 2007, pp. 366-372. doi:10.1016/j.vetmic.2007.01.027
[23] B.-A. Tenhagen, A. Fetsch, B. Stührenberg, G. Schleuter, B. Guerra, J. A. Hammerl, S. Hertwig, J. Kowall, U. K?mpe, A. Schroeter, J. Br?unig, A. K?sbohrer and B. Appel, “Prevalence of MRSA Types in Slaughter Pigs in Different German Abattoirs,” Veterinary Record, Vol. 165, No. 20 2009, pp. 589-593. doi:10.1136/vr.165.20.589
[24] E. van Duijkeren, R. Ikawaty, M. J. Broekhuizen-Stins, M. D. Jansen, E. C. Spalburg, A. J. de Neeling, J. G. Allaart, A. van Nes, J. A. Wagenaar and A. C. Fluit, “Transmission of Methicillin-Resistant Staphylococcus aureus Strains between Different Kinds of Pig Farms,” Veterinary Microbiology, Vol. 126, No. 4, 2008, pp. 383-389. doi:10.1016/j.vetmic.2007.07.021
[25] G. Normanno, M. Corrente., G. La Salandra, A. Dambrosio, N. C. Quaglia, A. Parisi, G. Greco, A. L. Bellacicco, S. Virgilio and G. V. Celano, “Methicillin-Resistant Staphylococcus aureus (MRSA) in Foods of Animal Origin Product in Italy,” International Journal of Food Microbiology, Vol. 117, No. 2, 2007, pp. 219-222. doi:10.1016/j.ijfoodmicro.2007.04.006
[26] A. J. Saiful, M. Mastura, S. Zarizal, M. I. Mazurah, M. Shuhaimi and A. M. Ali, “Detection of Methicillin-Resistant Staphylococcus aureus Using mecA/nuc Genes and Antibiotic Susceptibility Profile of Malaysian Clinical Isolates,” World Journal of Microbiology and Biotechnology, Vol. 22, No. 12, 2006, pp. 1289-1294. doi:10.1007/s11274-006-9174-9
[27] G. de Luca, F. Zanetti and S. Stampi, “Short Communication: Staphylococcus aureus in Dairy Products in the Bologna Area,” International Journal of Food Microbiology, Vol. 35, No. 3, 1997, pp. 267-270. doi:10.1016/S0168-1605(96)01229-9
[28] T. F. Jones, M. E. Kellum, S. S. Porter, M. Bell and W. Schaffner, “An Outbreak of Community Acquired Foodborne Illness Caused by Methicillin-Resistant Staphylococcus aureus,” Emerging Infectious Diseases, Vol. 8, No. 1, 2002, pp. 82-84. doi:10.3201/eid0801.010174
[29] J. Kluytmans, W. van Leeuwen, , W. Goessens, R. Hollis, S. Messer, L. Herwaldt, H. Bruining, M. Heck, J. Rost, N. van Leeuwen, A. van Belkum and H. Verbrugh, “Food-Initiated Outbreak of Methicillin-Resistant Staphylococcus aureus Analyzed by Pheno- and Genotyping,” Journal of Clinical Microbiology, Vol. 33, No. 5, 1995, pp. 1121-1128.

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