A Statistical Approach to Optimize Xylitol Production by Debaryomyces nepalensis NCYC 3413 in Vitro


Debaryomyces nepalensis NCYC 3413, halotolerant yeast isolated from rotten apple, was capable of utilizing components of hemicellulose hydrolysate such as glucose, galactose, mannose, xylose and arabinose. The organism utilizes xylose as a sole carbon source and produces xylitol. The Plackett-Burman design was applied to determine the specific medium components affecting xylitol production and found that xylose, K2HPO4, and ZnSO4 were critical in augmenting xylitol production. These significant parameters were further optimized using response surface methodology. The optimum concentrations of xylose, K2HPO4, and ZnSO4 were found to be 100 g/l, 10.6 g/l and 8.9 mg/l respectively. Under these optimal conditions the xylitol production increased from 27 g/l to 36 g/l with a yield of 0.44 g/g (57% increase in total yield). In addition, formation of the by product (glycerol) was decreased under optimal conditions.

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H. Kumdam, S. Murthy and S. Gummadi, "A Statistical Approach to Optimize Xylitol Production by Debaryomyces nepalensis NCYC 3413 in Vitro," Food and Nutrition Sciences, Vol. 3 No. 8, 2012, pp. 1027-1036. doi: 10.4236/fns.2012.38136.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] X. Chen, “Microbial and Bioconversion Production of D-Xylitol and Its Detection and Application,” International Journal of Biological Sciences, Vol. 6, No. 7, 2010, pp. 834-844. doi:10.7150/ijbs.6.834
[2] R. L. Plackett and J. P. Burman, “The Design of Optimum Multifactorial Experiments,” Biometrika, Vol. 33, No. 4, 1946, pp. 305-325. doi:10.2307/2332195
[3] J. M. Dominguez, C. S. Gong and G. T. Tsao, “Production of Xylitol from D-Xylose by Debaryomyces hansenii,” Applied Biochemistry and Biotechnology, Vol. 63-65, 1997, pp. 117-127. doi:10.1007/BF02920418
[4] F. K. Agbogbo, G. Coward-Kelly, M. Torry-Smith and K. S. Wenger, “Fermentation of Glucose/Xylose Mixtures Using Pichia Stipitis,” Process Biochemistry Vol. 41, No. 11, 2006, pp. 2333-2336. doi:10.1016/j.procbio.2006.05.004
[5] L. Preziosi-Belloy, V. Nolleau, and J. M. Navarro, “Fermentation of Hemicellulosic Sugars and Sugar Mixtures to Xylitol By Candida Parapsilosis,” Enzyme Microbial Technology, Vol. 21, 1997, pp. 124–129. doi:10.1016/S0141-0229(96)00247-5
[6] B. C. Saha “Hemicellulose Bioconversion,” Journal of Industrial Microbiology and Biotechnology, Vol. 30, 2003, pp. 279-291. doi:10.1007/s10295-003-0049-x
[7] S. Sanchez, V. Bravo, E. Castro, A. J. Moya and F. Camacho, “The Fermentation of Mixtures of D-Glucose and D-Xylose by Candida shehatae, Pichia stipitis and Pachysolen tannophilus to Produce Ethanol,” Journal of Chemical Technology and Biotechnology, Vol. 77, No. 6, 2002, pp. 641-648. doi:10.1002/jctb.622
[8] P. Nigam and D. Singh, “Processes of Fermentative Production of Xylitol—A Sugar Substitute,” Process Biochemistry, Vol. 30, No. 2, 1995, pp. 117-124. doi:10.1016/0032-9592(95)80001-8
[9] S. N. Gummadi and D. S. Kumar, “Pectin Lyase and Pectate Lyase from Debaryomyces nepalensis Isolated from Apple,” Research Journal of Microbiology, Vol. 1, No. 2, 2006, pp. 152-159. doi:10.3923/jm.2006.152.159
[10] S. Kumar and S. N. Gummadi, “Metabolism of Glucose and Xylose as Single and Mixed Feed in Debaryomyces nepalensis NCYC 3413: Production of Industrially Important Metabolites,” Applied Microbiology and Biotechnology Vol. 89, No. 5, 2011, pp. 1405-1415. doi:10.1007/s00253-010-2997-1
[11] D. Kandelman and G. Gagnon, “A 24-Month Clinical Study of the Incidence and Progression of Dental Caries in Relation to Consumption of Chewing Gum Containing Xylitol in School Preventive Programs,” Journal of Dental Research, Vol. 69, No. 11, 1990, pp. 1771-1775. doi:10.1177/00220345900690111201
[12] P. T. Mattila, M. J. Svanberg, T. Jamsa and M. L. E. Knuuttila, “Improved Bone Biomechanical Properties in Xylitol-Fed Aged Rats,” Metabolism, Vol. 51, No. 1, 2002, pp. 92-96.
[13] P. T. Mattila, M. J. Svanberg, T. Jamsa and M. L. E. Knuuttila, “Increased Bone Volume and Bone Mineral Content in Xylitol Fed Rats,” Gerentology, Vol. 47, No. 6, 2001, pp. 300-305. doi: 10.1159/000052818
[14] S. Kumar and S. N. Gummadi, “Purification and Biochemical Characterization of a Moderately Halotolerant NADPH Dependent Xylose Reductase from Debaryomyces nepalensis NCYC 3413,” Bioresource Technology, Vol. 102, No. 20, 2011, pp. 9710-9717. doi:10.1016/j.biortech.2011.07.030
[15] A. Maguire and A. J. Rugg-Gunn, “Xylitol and Caries Prevention—Is It a Magic Bullet?” British Dental Journal, Vol. 194, No. 8, 2003, pp. 429-436. doi:10.1038/sj.bdj.4810022
[16] S. S. Silva and A. S. Afschar, “Microbial Production of Xylitol from D-Xylose Using Candida tropicalis,” Bioprocess Engineering, Vol. 11, 1994, pp. 129-134. doi:10.1007/BF00518734
[17] R. Ylikahri, “Metabolic and Nutritional Aspects of Xylitol,” In: C. O. Chicester, Ed., Advances in Food Research, Vol. 25, Academic Press, Inc., New York, 1979, pp. 159- 180.
[18] B. J. Naveena, M. Altaf, K. Bhadriah and G. Reddy, “Selection of Medium Components by Plackett-Burman Design for Production of l(+) Lactic Acid by Lactobacillus amylophilus GV6 in SSF Using Wheat Bran,” Bioresource Technology, Vol. 96, No. 4, 2005, pp. 485-490. doi:10.1016/j.biortech.2004.05.020
[19] T. Ayse and E. Mubeccel, “Use of Experimental Design Method to Investigate Metal Ion Effects in Yeast Fermentations,” Journal of Chemical Technology and Biotechnology, Vol. 82, No. 1, 2007, pp. 11-15. doi: 10.1002/jctb.1616
[20] S. S. Dash and S. N. Gummadi, “Enhanced Biodegradation of Caffeine by Pseudomonas sp. Using Response Surface Methodology,” Biochemical Engineering Journal, Vol. 36, No. 3, 2007, pp. 288-293. doi:10.1016/j.bej.2007.03.002
[21] S. N. Gummadi, K. B. Ganesh and D. Santhosh, “Enhanced Degradation of Caffeine by Immobilized Cells of Pseudomonas sp. in Agar-Agar Matrix Using Statistical Approach,” Biochemical Engineering Journal, Vol. 44, No. 2-3, 2009, pp. 136-141. doi:10.1016/j.bej.2008.11.010
[22] S. N. Gummadi and D. S. Kumar, “Optimization of Chemical and Physical Parameters Affecting the Activity of Pectin Lyase and Pectate Lyase from Debaryomyces nepalensis: A Statistical Approach,” Biochemical Engineering Journal, Vol. 30, 2006, pp. 130-137. doi:10.1016/j.bej.2006.02.014
[23] R. M. A. Abedin and H. M. Taha, “Antibacterial and Antifungal Activity of Cyanobacteria and Green Microalgae. Evaluation of Medium Components by Placket- Burman Design for Antimicrobial Activity of Spirulina platensis,” Global Journal of Biotechnology and Biochemistry, Vol. 7, No. 3, 2008, pp. 22-31.
[24] C. Xiong, C. Shouwen, S. Ming and Y. Ziniu, “Medium Optimization by Response Surface Methodology for Poly-g-Glutamic Acid Production Using Dairy Manure as the Basis of a Solid Substrate,” Applied Microbiology and Biotechnology, Vol. 69, 2005, pp. 390-396. doi:10.1007/s00253-005-1989-z
[25] S. Yeruva, S. Mantha, A. Tirumalaraju and S. R. Rokkam, “Screening of Medium Components for Polyribosyl Ribitol Phosphate Production by Haemophilus influenzae Type-B Using Plackett-Burman Design,” Cell and Tissue Research, Vol. 10, No. 3, 2010, pp. 2349-2352.
[26] Y. Li, Z. Zhang, Z. Lei, Y. Yang, M. Utsumi and N. Sugiura, “Influence of Metal Addition on Ethanol Production with Pichia stipitis ATCC 58784,” Journal of Industrial Microbiology and Biotechnology, Vol. 36, 2005, pp. 491-497. doi:10.1007/s10295-008-0518-3
[27] B. R. Gibson, “Improvement of Higher Gravity Brewery Fermentation via Wort Enrichment and Supplementation,” Journal of Institute of Brewing, Vol. 117, No. 3, 2011, pp. 268-284.
[28] H. Ling, K. Chen, J. Ge and W. Ping, “Statistical Optimization of Xylitol Production from Corncob Hemicellulose Hydrolysate by Candida tropicalis HDY-02,” New Biotechnology, Vol. 28, 2011, pp. 673-678. doi:10.1016/j.nbt.2010.05.004
[29] Y. Xiao-Binu, N. Joo-Heon, S. Y. Hyun and K. Yoon-Mo, “Optimization of Cellulase Production in Batch Fermentation by Trichoderma reesei,” Biotechnology and Bioprocess Engineering, Vol. 3, 1998, pp. 44-47.
[30] E. Vandeska, S. Amartey, S. Kuzmanova and T. Jeffries, “Effects of Environmental Conditions on Production of Xylitol by Candida boidinii,” World Journal of Microbiology and Biotechnology, Vol. 11, No. 2, 1995, pp. 213- 218. doi:10.1007/BF00704652
[31] V. Meyrial, J. P. Delgenes, R. Moletta and J. M. Navarro, “Xylitol Production from D-Xylose by Candida Guillermondii: Fermentation Behaviour,” Biotechnology Letters, Vol. 13, No. 4, 1991, pp. 281-286.
[32] W. M. Jurick II, I. Vico, V. L. Gaskins, K. A. Peter, E. Park, W. J. Janisiewicz and W. S. Conway, “Carbon, Nitrogen and pH Regulate the Production and Activity of a Polygalacturonase Isozyme Produced by Penicillium expansum,” Archives of Phytopathology and Plant Protection, Vol. 45, No. 9, 2012, pp. 1-14. doi:10.1080/03235408.2012.657893.
[33] J. A. Rollins and M. B. Dickman, “pH Signaling in Sclerotina sclerotiorum: Identification of a pacC/RIM1 Homolog,” Applied and Environmental Microbiology, Vol. 67, No. 1, 2001, pp. 75-81.
[34] A. Converti and J. M. Dominguez, “Influence of Temperature and pH on Xylitol Production from Xylose by Debaryomyces hansenii,” Biotechnology and Bioengineering, Vol. 75, No. 1, 2001, pp. 39-45. doi:10.1016/j.procbio.2005.08.019.

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