Enumeration of microbial contaminants in sachet water: a public health challenge

Narasimhan Banu, Himabindu Menakuru
DOI: 10.4236/health.2010.26086                

Abstract

Accessibility and availability of fresh clean water is a key to sustainable development and essential element in health, food production and poverty reduction. In the present study, we have collected water sachet containing CM/L number and they were analysed for physical and bacteriological nature. The organisms isolated in this study were Proteus mirabilis, Klebsiella pneumoniae, Pseudomonas vesicularis and Pseudomonas aeruginosae. The harmful effects of these isolates were evidenced by antibiotic resistance, heavy metal tolerance and antibacterial activity. They were resistant to the antibiotics like amoxiclav, methicillin, chloramphenicol and streptomycin. They showed tolerance to the heavy metals at 5 mM conc. except for lead. For antibacterial activity, they were tested against human pathogens Klebsiella pnemoniae, Proteus mirabilis, Micrococcus leuteus and Salmonella paratyphium. But at the same time these organisms could be exploited for the industrial production of amylase, protease and cellulase.

Keywords

Sachet Water; Bacterial Contaminants; Pathogens; Industrially Useful Bacteria

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Archimpong, E.Q. (1976) Typhoid ideal perforation: Why such Mortalities. British Journal of Surgery, 63, 317-321.
[2]	Ajao, O.G. (1984) Typhoid perforation: Factors influencing mortality and morbidity. International Surgery, 67(4), 317-319.
[3]	Badejo, O.O. and Arigbabu, A.O. (1980) Treatment of Typhoid perforation and peritoneal irrigation and comparative study. Gut, 21(2), 141-145.
[4]	Olayemi, A.B. (1999) Microbial potability of bottled and packaged drinking water hawked in Ilorin metropolis. International journal of Environmental Health Research, 9(3), 245-248.
[5]	Franco, R.M.B. and Cantusio, N.R. (2000) Occurrence of cryptosporidial oocytes and Giardia cysts in Bottled mineral water commercialized in the city of Campinas, State of Sao Paulo, Brazil. Memórias do Instituto Os- waldo Cruz, Riode Janero, 97(2), 205-207.
[6]	Adekunle, L.V., Sridhar, M.K.C., Ajayi, A.A., Oluwade, P.A. and Olawuyi, J.F. (2004) An assessment of the health and social economic implication of sachet water in Ibadan Nigeria. African journal of Biomedical Research, 7(1), 5-8.
[7]	Robertson, L.J., Forbeeg, T. Hermansen, L. Gjerde, B.K. Alsvag, J.O. and Langeland, N. (2006) Cryptosporidium parvum infection in Bergen, Norway, during an extensive out break of water borne Giardiasis. Applied and Environmental Microbiology, 72(3), 2218-2220.
[8]	Holt, J.G. (1994) Bergey’s manual of determinative bacteriology. 9th Edition, Lippincott Williams and Wilkins, Baltimore.
[9]	Ademoroti, C.M.A. (1996) Standard method for water and effluents analysis. Folludex Press Ltd., Ibadan, Nigeria.
[10]	Aganda, O.A. (1998) Result of baseline studies on water supply & sanitation conducted by federal Ministry of water resources & rural development, Nigeria, UNICEF assisted National water supply & sanitation monitoring programme–an overview. FGN–UNICEF WEB Review meeting, Ibadan, 1-14.
[11]	Rutz, D. (1996) Pure water vending machines may not be so pure. Food & Health, Cable News Network.