Sterilization of Hibiscus rosa-sinensis L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on In Vitro Direct Regeneration


This paper reports on the effects of organic ingredients in facilitating direct shoot regeneration from nodal explants of Hibiscus rosa-sinensis L. This paper also compares the sterilization conditions for 3 types of explants (node, internode, and shoot tip) harvested from an open field. The optimized sterilization conditions for the explants were 40% Clorox- 20 min exposure, 10% Clorox-15 min exposure, and 5% Clorox-40 min exposure for the node, internode and shoot tip, respectively. In the direct shoot regeneration using the nodal explants, we found MS medium containing 40 g/L sucrose, 0.3% (w/v) activated charcoal, and supplementations with myo-inositol, thiamine and nicotinic acid were suitable. The in vitro shoot survival rate was 30% with a mean leaf numbers of 2.68 produced, and a mean leaf length of 1.71 cm achieved after 5 weeks of culture on the modified medium.

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C. Dar, J. Abdullah, P. Namasivayam and S. Roowi, "Sterilization of Hibiscus rosa-sinensis L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on In Vitro Direct Regeneration," American Journal of Plant Sciences, Vol. 3 No. 6, 2012, pp. 791-798. doi: 10.4236/ajps.2012.36095.

Conflicts of Interest

The authors declare no conflicts of interest.


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