Increased Apoptosis in Chorionic Trophoblasts of Human Fetal Membranes with Labor at Term


Objective: To determine the association of apoptosis in the layers of human fetal membranes distal to rupture site with labor at term. Study Design: Fetal membranes were collected from elective cesarean sections (n = 8) and spontaneous vaginal deliveries (n = 8) at term. The extent of apoptosis within fetal membrane layers was determined using terminal deoxynucleotidyl transferase deoxy-UTP-nick end labeling (TUNEL) assay and western blots for pro-apoptotic active caspase-3 and anti-apoptotic Bcl-2. Results: Apoptotic index in chorionic trophoblasts of membranes distal to rupture site obtained after vaginal delivery was 3-fold higher than those obtained from elective cesarean (11.57% ± 4.98% and 4.05% ± 2.4% respectively; p = 0.012). The choriodecidua layers after vaginal deliveries had higher expression of the pro-apoptotic active caspase-3 and less expression of the anti-apoptotic Bcl-2 than those obtained from elective cesarean sections. Conclusions: Labor at term is associated with increased apoptosis in chorionic trophoblast cells of human fetal membranes distal to rupture site.

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H. M. Harirah, M. A. Borahay, W. Zaman, M. S. Ahmed, I. G. Hager and G. D. V. Hankins, "Increased Apoptosis in Chorionic Trophoblasts of Human Fetal Membranes with Labor at Term," International Journal of Clinical Medicine, Vol. 3 No. 2, 2012, pp. 136-142. doi: 10.4236/ijcm.2012.32027.

Conflicts of Interest

The authors declare no conflicts of interest.


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