Leaf Wand for Measuring Chlorophyll Fluorescence on Cylindrical Leaves and Its Application on Juncus roemerianus (Black Needlerush)
Patrick D. Biber
DOI: 10.4236/ajps.2012.31007   PDF    HTML     7,545 Downloads   11,623 Views   Citations


Chlorophyll fluorescence is a well established technique to rapidly and non-invasively determine photosynthesis parameters in plant leaves. It can be used in both laboratory and field settings, and frequently dark-adaptation of a leaf sample is called for. In the field, this can be accomplished on flat leaves using standard leaf clips supplied by instrument manufacturers. However, not all plant leaves are flat, many are cylindrical or otherwise three-dimensional in shape. The standard leaf clip does not close fully on three-dimensional leaves, therefore, does not allow the sample to be properly dark adapted in the field. A new leaf “wand” was developed that can be slipped over an entire cylindrical leaf or culm of rushes and sedges for both light- and dark-adapted measurements. This new leaf wand is compared to the standard leaf clip (DLC-8) using a Walz mini-PAM on Juncus roemerianus(Black needlerush). Results indicate that dark-adapted yield measurements are not significantly different between leaf clips, while light-adapted yields are higher with the leaf wand. The potential sources of difference in the optical path of the excitation light and fluorescence return are discussed and compared between leaf clips. Construction of specialized leaf wands should be considered for any leaves are not flat and therefore that do not fit the standard leaf clip for complete dark-adaptation under field conditions.

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P. Biber, "Leaf Wand for Measuring Chlorophyll Fluorescence on Cylindrical Leaves and Its Application on Juncus roemerianus (Black Needlerush)," American Journal of Plant Sciences, Vol. 3 No. 1, 2012, pp. 75-83. doi: 10.4236/ajps.2012.31007.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] C. Critchley and R. M. Smilie, “Leaf Chlorophyll Fluorescence as an Indicator of Photoinhibition in Cucumis sativus L.,” Australian Journal of Plant Physiology, Vol. 8, No. 2, 1981, pp. 133-141. doi:10.1071/PP9810133
[2] M. Havaux and R. Lannoye, “Chlorophyll Fluorescence Induction: A Sensitive Indicator of Water Stress in Maize Plants,” Irrigation Science, Vol. 4, 1983, pp. 147-151.
[3] J. R. Bowyer, P. Camilleri and W. F. J. Vermaas, “Photosystem II and Its Interaction with Herbicides,” In: N. R. Baker and M. P. Percival, Eds., Herbicides, Topics in Photosynthesis, Elsevier, Amsterdam, 1991, pp. 27-85.
[4] D. L. Filiault and J. C. Stier, “The Use of Chlorophyll Fluorescence in Assessing the Cold Tolerance of Three Turfgrass Species,” Wisconsin Turfgrass Research, Vol. 16, 1999, pp. 109-110.
[5] H. R. Nordenkampf, S. P. Long, N. R. Baker, G. Oquist, U. Schreiber and E. G. Lechner, “Chlorophyll Fluorescence as a Probe of the Photosynthetic Competence of Leaves in the Field: A Review of Current Instrumentation,” Functional Ecology, Vol. 3, No. 4, 1989, pp. 497-514. doi:10.2307/2389624
[6] K. Maxwell and G. N. Johnson, “Chlorophyll Fluorescence: A Practical Guide,” Journal of Experimental Botany, Vol. 51, No. 345, 2000, pp. 659-668. doi:10.1093/jexbot/51.345.659
[7] B. Genty, J. Briantais and N. Baker, “The Relationship between the Quantum Yield of Photosynthetic Electron Transport and Quenching of Chlorophyll Fluorescence,” Biochemica et Biophysica Acta, Vol. 990, No. 1, 1989, pp. 87-92. doi:10.1016/S0304-4165(89)80016-9
[8] U. Schreiber, H. Horman, C. Neubauer and C. Klughammer, “Assessment of Photosystem II Photochemical Quantum Yield by Chlorophyll Fluorescence Quenching Analysis,” Australian Journal of Plant Physiology, Vol. 22, No. 2, 1995, pp. 209-220. doi:10.1071/PP9950209
[9] E. D. Schulze and M. M. Caldwell, “Ecophysiology of Photosynthesis,” Springer, Berlin, 1990.
[10] G. H. Krause, and E. Weis, “Chlorophyll Fluoresence and Photosynthesis: The Basics,” Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 42, 1991, pp. 313-349. doi:10.1146/annurev.pp.42.060191.001525
[11] K. Rohacek and M. Bartak, “Technique of the Modulated Chlorophyll Fluorescence: Basic Concepts, Useful Parameters, and Some Applications,” Photosynthetica, Vol. 37, No. 3, 1999, pp. 339-363. doi:10.1023/A:1007172424619
[12] R. K. Godfrey and J. W. Wooten, “Aquatic and Wetlands Plants of the Southeastern United States,” University of Georgia Press, Athens, 1979.
[13] A. D. Richardson, G. P. Berlyn and T. G. Gregoire, “Spectral Reflectance of Picea rubens (Pinaceae) and Abies balsamea (Pinaceae) Needles along an Elevational Gradient, Mt. Moosilauke, New Hampshire, USA,” American Journal of Botany, Vol. 88, No. 4, 2001, pp. 667-676. doi:10.2307/2657067
[14] T. Brodribb and R. S. Hill, “Light Response Characteristics of a Morphologically Diverse Group of Southern Hemisphere Conifers as Measured by Chlorophyll Fluorescence,” Oecologia, Vol. 110, No. 1, 1997, pp.10-17. doi:10.1007/s004420050127
[15] P. D. Biber, “Evaluating a Chlorophyll Content Meter on Three Coastal Wetland Plant Species,” Journal of Agricultural, Food, and Environmental Sciences, Vol. 1, No. 2, 2007, pp. 1-11.
[16] G. Naidoo and J. Kift, “Responses of the Saltmarsh Rush Juncus Kraussii to Salinity and Waterlogging,” Aquatic Botany, Vol. 84, No. 3, 2006, pp. 217-225. doi:10.1016/j.aquabot.2005.10.002
[17] K. L. Castro-Esau, G. A. Sanchez-Azofeifa, B. Rivard, S. J. Wright and M. Quesada, “Variability in Leaf Optical Properties of Mesoamerican Trees and the Potential for Species Classification,” American Journal of Botany, Vol. 93, No. 4, 2006, pp. 517-530. doi:10.3732/ajb.93.4.517
[18] A. D. Richardson, S. P. Duigan and G. P. Berlyn, “An Evaluation of Noninvasive Methods to Estimate Foliar Chlorophyll Content,” New Phytologist, Vol. 153, No. 1, 2002, pp. 185-194. doi:10.1046/j.0028-646X.2001.00289.x
[19] J. Markwell, J. Osterman and J. Mitchell, “Calibration of the Minolta SPAD-502 Leaf Chlorophyll Meter,” Photosynthesis Research, Vol. 46, No. 3, 1995, pp. 467-472. doi:10.1007/BF00032301
[20] M. N. Merzlyak, O. B. Chivkunova, T. V. Zhigalova and K. R. Naqvi, “Light Absorption by Isolated Chloroplants and Leaves: Effect of Scattering and Packaging,” Photosynthesis Research, Vol. 102, No. 1, 2009, pp. 31-41. doi:10.1007/s11120-009-9481-8
[21] T. C. Vogelmann and J. R. Evans, “Profiles of Light Absorption and Chlorophyll within Spinach Leaves from Chlorophyll Fluorescence,” Plant, Cell and Environment, Vol. 25, No. 10, 2002, pp. 1313-1323. doi:10.1046/j.1365-3040.2002.00910.x
[22] L. Eleuterius, “Vegetative Morphology and Anatomy of the Salt Marsh Rush, Juncus roemerianus,” Gulf Research Reports, Vol. 5, No. 2, 1976, pp. 1-10.
[23] D. W. Lee, S. F. Oberbauer, P. Johnson, B. Krishnapilay, M. Mansor, H. Mohamad and S. K. Yap, “Effects of Irradiance and Spectral Quality on Leaf Structure and Function in Seedlings of Two Southeast Asian Hopea (Dipterocarpaceae) Species,” American Journal of Botany, Vol. 87, No. 4, 2000, pp. 447-455. doi:10.2307/2656588
[24] J. Serodio, “Analysis of Variable Chlorophyll Fluorescence in Microphytobenthos Assemblages: Implications of the Use of Depth-Integrated Measurements,” Aquatic Microbial Ecology, Vol. 36, No. 2, 2004, pp. 137-152. doi:10.3354/ame036137

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