In Vitro Antibacterial and Antifungal Activity of Methanol, Chloroform and Aqueous Extracts of Origanum vulgare and Their Comparative Analysis
Zaman Ashraf, Aun Muhammad, Muhammad Imran, Ahmad Hussen Tareq
DOI: 10.4236/ijoc.2011.14037   PDF    HTML     6,892 Downloads   13,214 Views   Citations


The work reports antibacterial and antifungal activity of different solvent extracts of Origanum vulgare. The antimicrobial activity of methanol, chloroform and aqueous extracts were determined against nine different gram negative and gram positive bacterial strains and three fungal stains. The bacterial strains were Bacillus subtilis (ATCC 6633), Staphylococcus aureus (ATCC 29213), Micrococcus luteus (ATCC 9341), Pseudo-monas aeruginosa (ATCC 33347), Escherichia coli (ATCC 25922), Salmonella typhi (ATCC 19430), Shig-ella flexneri (ATCC 25929), Salmonella para typhi A (ATCC 9150) and Proteus mirabilis (ATCC 49565) and fungal strains were Aspergillus flavus, Aspergillus nigar and Aspergillus pterus. Agar well diffusion method was followed in this study. The comparative analysis of antibacterial activity reflects that among these three extracts, chloroform and methanol extracts shows promising result by exhibiting maximum anti-bacterial activity, whereas aqueous extract is not active against most of these strains. The analysis of anti-fungal activity reveals chloroform extract as most efficacious unlike methanol and Aqueous extracts.

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Ashraf, Z. , Muhammad, A. , Imran, M. and Tareq, A. (2011) In Vitro Antibacterial and Antifungal Activity of Methanol, Chloroform and Aqueous Extracts of Origanum vulgare and Their Comparative Analysis. International Journal of Organic Chemistry, 1, 257-261. doi: 10.4236/ijoc.2011.14037.

Conflicts of Interest

The authors declare no conflicts of interest.


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