Camalote Grass (Paspalum fasciculatum Willd) as a Sustainable Raw Material for the Production of Lignocellulosic Ethanol


The current trend of replacing a percentage of gasoline with ethanol has promoted the development of new processes for its production from lignocellulosic biomass. This work reports the production of ethanol from the Camalote grass (Paspalum fasciculatum Willd). The lignocellulosic biomass was subjected to acid hydrolysis at 125°C and 15 psi with H2SO4 concentrations at 5%, 10%, and 20%, obtaining an average of reducing sugars (pentoses and hexoses) from the hydrolyzed juice with 12.3%, 10%, and 17% Brix, respectively. The sugars were fermented using yeast of the Saccharomyces cerevisiae at 30°C for 48 hours. Finally, the ethanol was distilled at 78°C, and the average yields were obtained through analysis of variance with a 95% confidence level. The values indicate that there is a significant difference (p > 0.05), the Tukey study shows that all the % v/v averages are different from each other. For H2SO4 concentration at 5% (10.33 ± 2), H2SO4 at 10% (9.33 ± 1.8), and H2SO4 at 20% (6.33 ± 2). The acidity analysis for the ethanol obtained from each treatment gave a value of 1.8 mg/L of acetic acid in all cases.

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May-Reyes, M. , Valerio-Cárdenas, C. , Bolio-López, G. , Hernández-Villegas, M. , Velázquez-Carmona, M. and Cruz-Burelo, P. (2024) Camalote Grass (Paspalum fasciculatum Willd) as a Sustainable Raw Material for the Production of Lignocellulosic Ethanol. Journal of Sustainable Bioenergy Systems, 14, 23-33. doi: 10.4236/jsbs.2024.142002.

1. Introduction

Ethanol (C2H6O) due to its chemical composition has the characteristics to be used as a fuel and oxygenate gasoline. During the period of oil shortages between 1973 and 1979, first-generation ethanol from sugarcane juice and corn grains was used as fuel. Since then, Brazil and the United States have used ethanol blended with gasoline at 10%, called gasohol (E10). This blend works well in conventional cars without the need to adjust the carburetor [1] . A theoretical study conducted by Castillo-Hernández (2012), it was demonstrated that CO2 emissions decrease by 7% using gasohol with positive energy balances [2] . Other benefits of substituting ethanol for gasoline include reducing the presence of harmful aromatic compounds, replacing oxygenates such as methyl tert-butyl ether (MTBE), and reducing PM10 particle emissions, thereby improving air quality.

However, in Latin American countries, the use of corn and sugarcane is compromised by food security concerns, making them unsuitable as raw materials for ethanol production. Consequently, second and third-generation ethanol [3] are being produced, primarily obtained from lignocellulosic biomass (Table 1).

3rd Generation Ethanol

At the National Ethanol Conference 2022, grasses were considered affordable materials for ethanol production since they do not require subsidies, affect diversity, or contribute to deforestation [4] . Grasses have lignocellulosic biomass with a complex structure, where hemicellulose and lignin form a true physical barrier to cellulolytic enzyme penetration. Depending on the variety of grasses, they can contain from 5% to 18% lignin, from 25% to 43% hemicellulose, and from 29% to 50% cellulose [5] , so the processes for ethanol production from grass biomass must be adjusted according to the characteristics of these components [6] . Table 2 shows a list of different grasses used under various pretreatments for ethanol production.

Table 1. Generation feedstocks for bioethanol yield.

Table 2. Generation feedstocks for bioethanol yield.

Figure 1. Camalote grass (Paspalum fasciculatum Willd).

In this regard, considering the lignocellulosic biomass of grasses as a promising raw material for ethanol production, this work presents the use of lignocellulosic biomass from Camalote grass to produce ethanol. Camalote grass (Figure 1) belonging to the Poaceae family, is a perennial, fast-growing, and invasive grass that displaces cultivated pastures, hence considered a weed. Moreover, it is not utilized as livestock feed [13] . It is abundant in the southeast of Mexico, extending to Argentina, Uruguay, and the Antilles, thus holding great potential as a raw material for ethanol production due to its high availability, easy access, and low cost.

2. Materials and Methods

2.1. Sample Collection

1 m2 (3.9 kg) of camalote grass (Paspalum fasciculatum Willd) was collected in the vicinity of the Universidad Popular de la Chontalpa, with coordinates 17.959 North, −93.364 West. The grass was longitudinally cut into sizes of 5 ± 1 cm to facilitate handling. Subsequently, it was air-dried for 3 days and then dried at 105˚C until reaching a constant weight.

% m o i s t u r e = ( m i m f ) 100 (1)

where mi is the initial weight of the plant material (g) and mf is the final weight of the plant material (g).

2.2. Extractives Released

100 g of camalote grass were placed in a 1 L beaker and 700 mL of acetone were added. It was heated to boiling for 20 minutes with continuous stirring. Subsequently, it was allowed to cool, and the resulting plant material was filtered. It was then dried at 105˚C until reaching a constant weight.

% e x t r a c t i v e s = m i m f m i × 100 (2)

where mi is the initial weight of the plant material (g) and mf is the final weight of the plant material (g).

2.3. NaClO Treatment

To 100 g of the material without extractives, 500 mL of commercial NaClO was added, and heated to boiling for 1 hour. It was then cooled and washed until neutral pH. The resulting material was air-dried for 2 days and subsequently dried at a controlled temperature of 105˚C until constant weight.

2.4. Determination of Percentage of Crystallinity

The diffraction patterns were collected using a D8 Advance Bruker with CuKα radiation (α = 1.5406 Å and energy 8.047 keV), in the range of 2θ = 7 - 60 with a 0.02 step size and an acquisition time of 5 s/step. The crystallinity percentage was calculated with Equation (2) based on the method reported by Segal (1959) [14] .

CrI ( % ) = ( 1 I A M I 002 ) × 100 (3)

where I002 is the maximum intensity of the crystalline peak at 22˚, and IAM = is the minimum intensity of the crystalline peak for cellulose I.

2.5. Acid Hydrolysis

10 g of pretreated material were placed in a 250 mL Pyrex flask and 100 mL of a 5% H2SO4 solution was added. This procedure was repeated varying the concentration of H2SO4 to 10% and 15%. Hydrolysis was carried out at 125˚C and 15 psi for 2 hours. Subsequently, the hydrolyzed juice was separated by filtration. The % Brix measurement was performed using a portable Anpro refractometer, by placing a drop on the prism surface and reading the scale.

2.6. Fermentation of Reducing Sugars

An experimental design was conducted by varying the amount of Saccharomyces cerevisiae yeast (Fermentis®) to 0.5, 1, and 2 g 100 mL of hydrolyzed juice was placed in an Erlenmeyer flask, the pH was adjusted to 5 with NaOH 0.1 N, yeast was added, and it was allowed to ferment under anaerobic conditions for 48 hours at a temperature of 30˚C ± 1˚C.

2.7. Ethanol Distillation

The fermented product was placed in a simple distillation setup and heated to 78 - 80˚C for 3 hours. The % v/v of ethanol was measured using a portable refractometer for alcohol and distilled spirits with a range of 0 - 80% alcohol, by placing a drop on the prism surface and reading the scale.

2.8. Determination of the Acidity (Acetic Acid) of Ethanol

It was carried out according to the Mexican Official Standard NOM-V-15-S-1980. 25 mL of bioethanol were placed in a porcelain dish, heated in a water bath to dryness, and then transferred to an oven at a temperature of 105˚C for 30 minutes. Subsequently, 50 mL of absolute alcohol was added to the residue left in the dish, and the resulting solution was poured over 250 mL of freshly boiled, cold water neutralized with NaOH 0.1 N and phenolphthalein as an indicator. The resulting solution was titrated with NaOH 0.1 N, using the indicator added to the water for neutralization. The acidity was expressed in mg of acetic acid per 100 mL referred to anhydrous alcohol through Equation (4).

FA = V × N × 60 × 100 M × 100 D . A . R (4)

The fixed acidity (FA) expressed in mg of acetic acid per 100 mL referred to anhydrous alcohol; V is the volume of the NaOH 0.1 N solution spent for the titration of the sample in mL; N is the normality of NaOH; 60 represents the meq of CH3COOH in mg; M is the volume of the alcohol sample, in mL; and D.A.R. is the actual alcoholic degree of the sample on the Gay-Lussac scale.

2.9. Statistic Analysis

With the experimental data of % Brix and % ethanol, the average yield of each treatment was calculated using the RM ANOVA (repeated measures ANOVA) program, using biomass quantity as the fixed factor and H2SO4 concentration as the random factor. The size of the arithmetic mean = 3. The average values were compared by applying Analysis of Variance with a 95% Confidence Interval for the mean, and the Tukey statistical test was applied.

3. Results and Discussion

3.1. Characterization of Camalote Grass

In the biomass’s own composition, cellulose, hemicellulose, lignin are present in larger quantities, while other organic compounds know extractives are found in low concentrations in the bark, leaves, needles, exudates, branches, flowers, fruits, and seeds, contributing to the organoleptic characteristics (flavor, odor, color) of the organic material. The amount of extractives varies according to the

Table 3. Grass characterization during pretreatment.

species, geographical location, and time of year. Among the extractables are phenolic compounds of molecular weight that are lignin precursors, as well as aromatic aldehydes and ketones. A material balance was carried out during the biomass pretreatment, and Table 3 shows the characterization of the solid fraction of the grass. The values obtained are close to those reported for Maralfalfa grass (Pennisetum glaucum) at cutting frequencies of 90 to 180 days [15] .

3.2. Effect of NaClO on Camalote Grass

During the NaClO treatment, lignin and hemicellulose were removed, as confirmed by X-ray analysis. In Figure 2(a), the diffraction patterns for natural camalote grass are shown. The intensity of the amorphous zone IAM was located at 2θ = 10.2˚, and the intensity of the crystalline zone I002 at 2θ = 22.6˚. In Figure 2(b), the camalote grass with NaClO is shown. The intensity of the amorphous zone IAM was located at 2θ = 11.2˚, and the intensity of the crystalline zone I002 at 2θ = 22.7˚, characteristic signals for cellulose I. The signal at 45.4˚ corresponds to the inorganic part of the cellulose [16] . Natural grass exhibits 42% crystallinity, and after treatment with NaClO, an increase in crystallinity to 50% is observed, attributed to the dissolution of amorphous regions (lignin and hemicellulose) in lignocellulosic biomass [17] .

3.3. Effect of H2SO4 Concentration on % Brix

The degradation of cellulose to glucose was carried out through acid hydrolysis varying the concentration of H2SO4. The sugar content is commonly expressed as % Brix since there is a relationship with the sugar content. Table 4 shows the results of % Brix for each of the treatments. Higher conversion of cellulose to reducing sugars was achieved with concentrations of 5% and 20%. The use of diluted acid concentrations minimizes the formation of hydroxymethylfurfural, which is a toxic compound for yeast during the fermentation process [18] .

Figure 2. X-ray diffractograms. (a) Natural camalote grass, (b) Camalote grass treated with NaClO.

Table 4. Values obtained of % Brix.

Figure 3. % Brix with respect to acid hydrolysis concentration. Data are presented as means ± SEM.

The analysis of variance showed that there are statistical differences (p < 0.05) among the H2SO4 treatments (Figure 3). The treatment with a 20% acid concentration presented the highest average of 17% Brix, which was statistically superior to the treatments with 5% acid concentration of 12.3% Brix and 10% acid concentration of 10% Brix. The % Brix values obtained are within recommended ranges as a high content of soluble solids increases osmotic pressure affecting the yeast Saccharomyces cerevisiae, resulting in low ethanol production [19] .

3.4. Yield % v/v of Ethanol

Table 5 shows the ethanol yield obtained relative to the amount of fermentable sugars obtained in each treatment with sulfuric acid.

Table 5. Production of ethanol from Camalote grass.

Figure 4. Ethanol with respect to acid concentration. Data are presented as means ± SEM.

Figure 4 shows that all the averages are different from each other, according to the Tukey statistical test. The treatment with a 5% of H2SO4 concentration produced the highest average ethanol value of 10.3%, the treatment with 10% of H2SO4 produced 9.3% of ethanol, and the treatment with 20% of H2SO4 produced 6.3% of ethanol. It is established that higher acid concentrations decrease ethanol production, as it increases the formation of toxic agents for yeast [20] .

3.5. Estimation of Ethanol Production per Hectare

It is predicted for 2024 that ethanol consumption will be 134.5 billion liters, with Brazil being the largest producer, followed by the United States, the EU, and China [21] . Based on the results, the camalote grass produces 0.10 g ethanol/g of biomass, values comparable to the yields reported by Antonopoulou (2020) for lawn grass, which produced 0.108 g of ethanol/g of biomass; and by Yosuda (2013) for Napiergrass, which produced 0.174 g of ethanol/g of biomass. In 1 m2, 3900 g of grass is obtained, therefore, 390 g ethanol/m2 would be produced. So, ethanol production from camalote grass would contribute 3900000 liters of ethanol per hectare. This yield is economically viable considering that the cost of the raw material is zero.

Yieldethanol / m 2 = ( 3900gpasto 1m 2 ) ( 0 .10gethanol 1gpasto ) = 390gEthanol / m 2

Yieldethanol / 1h = ( 390gethanol 1m 2 ) ( 10000m 2 1h ) = 3900000gEthanol / h

Thereby, grasses are promising raw materials to substitute sugar cane and corn for ethanol production, as they are not affected by climatic changes. Unlike sugar cane and corn, which only thrive in tropical climates and their crops are affected in semi-arid regions, hail, and frosts.

Table 6. Fixed acidity of ethanol obtained from Camalote grass.

3.6. Fixed Acidity in Ethanol from Camalote Grass

Ethanol, to be used as fuel in gasoline, must meet certain chemical properties, including fixed acidity, which is associated with corrosiveness, taking into account the material from which the car engine is made. According to the United Nations, fuel ethanol must have a maximum acidity (acetic acid) of 30 mg/L, established by ASTM D1613 standard. The ethanol obtained from Camalote grass in the three studied H2SO4 treatments showed a total acidity value as acetic acid of 1.8 mg/L (Table 6), acceptable values for the specifications set by International and Brazilian Standards [22] .

4. Conclusion

In this research work, it was determined that the camalote grass (Paspalum fasciculatum Willd) under conditions of acid hydrolysis at low concentrations of H2SO4 achieves an average maximum of 17% reducing sugars and 10.3% lignocellulosic ethanol with a fixed acidity of 1.8 mg/L in 48 hours of fermentation. The results are promising to propose Camalote grass as an energy crop for lignocellulosic ethanol production, as it is economically viable, not an agricultural food crop, produced in large quantities, has zero cost, and does not require special care for its growth.


The authors thank the CCYTET for the financial support provided through the project “Evaluation of lignocellulosic biomass of camalote (Paspalum fasciculatum Willd) as a raw material for obtaining bioethanol” (PRODECTI-2022-01/054).

Conflicts of Interest

The authors declare no conflicts of interest regarding the publication of this paper.


[1] Andersen, V.F., Anderson, J.E., Wallington, T.J., Mueller, S.A. and Nielsen O.J. (2010) Vapor Pressures of Alcohol-Gasoline Blends. Energy & Fuels, 24, 3647-3654.
[2] Castillo-Hernández, P., Mendoza-Domínguez, A. and Caballero-Mata, P. (2012) Analysis of Physicochemical Properties of Mexican Gasoline and Diesel Reformulated with Ethanol. Ingeniería Investigación y Tecnología, 13, 293-306.
[3] Álvarez Maciel, C. (2009) Biocombustibles: Desarrollo histórico-tecnológico, mercados actuales y comercio internacional. Economía Informa, 35, 63-89.
[4] National Ethanol Conference 2022.
[5] Mohapatra, S., Mishra, S.S., Bhalla, P. and Thatoi, H. (2019) Engineering Grass Biomass for Sustainable and Enhanced Bioethanol Production. Planta, 250, 395-412.
[6] Tejada Benítez, L., Tejada Tovar, C., Villabona Ortiz, A., Tarón Dunoyer, A., Alvear, M., Castillo C., Henao, D., Marimón, W. and Madariaga, N. (2010) Producción de bioetanol a partir de la fermentación alcohólica de jarabes glucosados derivados de cáscaras de naranja y piña. Revista Educación en Ingeniería, 5, 120-125.
[7] Aiyejagbara, M.O., Aderemi, B.O., Ameh, A.O., Ishidi, E., Aiyejagbara. E.F., Ibeneme, U. and Olakunle, M.S. (2016) Production of Bioethanol from Elephant Grass (Pennisetum purpureum) Stem. International Journal of Innovative Mathematics, Statistics & Energy Policies, 4, 1-9.
[8] Cardona, E.M., Ríos, J.A., Peña, J.D. and Ríos, L.A. (2013) Pretratamiento Alcalino de Pasto Elefante (Pennisetum sp) y King Grass (Pennisetum hybridum) Cultivados en Colombia para la Producción de Bioetanol. Información Tecnológica, 24, 69-80.
[9] Torres Robles, R., Cano López, M.N. and Aburto Anell, J. (2018) Obtención de bioetanol a partir de pasto kikuyo (Pennisetum clandestinum). Revista Iberoamericana de Ciencias, 5, 1-23.
[10] Raud, M. and Kikas, T. (2020) Perennial Grasses as a Substrate for Bioethanol Production. Environmental and Climate Technologies, 24, 32-40.
[11] Antonopoulou, G. (2020) Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste. Molecules, 25, Article 2889.
[12] Yasuda, M., Takeo K., Nagai, H., Uto. T., Yui, T., Matsumoto, T., Ishii, Y. and Ohta, K. (2013) Enhancement of Ethanol Production from Napiergrass (Pennisetum purpureum Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment. Journal of Sustainable Bioenergy Systems, 3, 179-185.
[13] Chacón, J.C. and Gliessman, S.R. (1982) Use of the “Non-Weed” Concept in Traditional Tropical Agroecosystems of South-Eastern Mexico. Agro-Ecosystems, 8, 1-11.
[14] Segal, L., Creely, J.J. and Martin, A.E. (1959) An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-Ray Diffractometer. Textile Research Journal, 29, 786-794.
[15] Ventura Ríos, J., Honorato Salazar, J.M., Hernández Garay, A., Aburto Anell, J.A., Vaquera Huerta, H. and Enríquez Quiroz, J.F. (2017) Composición química y rendimiento de biomasa de maralfalfa para producción de bioetanol de segunda generación. Revista Mexicana de Ciencias Agrícolas, 8, 215-221.
[16] Rohaeti, E. and Irzaman, H. (2010) Production of Semiconductor Materials Silicon from Silica Rica Husk Waste as Alternative Silicon Sources. Materials Science and Technology, 1, 265-272.
[17] Melese, G., Tesfaye, G., Anteneh, B. and Tsige, G.-M. (2023) Extraction and Characterization of Cellulose and Microcrystalline Cellulose from Teff Straw and Evaluation of the Microcrystalline Cellulose as Tablet Excipient. Journal of Natural Fibers, 20, Article 2245565.
[18] Guarnizo Franco, A., Martínez Yépes, P.N. and Valencia Sánchez, H.A. (2009) Pretratamientos de la celulosa y biomasa para la sacarificación. Scientia et Technica, 15, 284-289.
[19] Vital López, P. and Larralde Corona, C.P. (2016) Respuestas metabólicas al estrés de levaduras de importancia industrial. Investigación y Ciencia, 24, 86-91.
[20] Uçar, G. and Balaban, M. (2003) Hydrolysis of Polysaccharides with 77% Sulfuric Acid for Quantitative Saccharification. Turkish Journal of Agriculture and Forestry, 27, 361-365.
[21] Cuevas-García. R. and Nava Bravo, I. (2023) Producción de combustibles renovables. Mundo Nano. Revista Interdisciplinaria En Nanociencias Y Nanotecnología, 16, 1e-50e.
[22] Gracida Rodríguez, J.N. and Perez-Diaz, B. (2014) Factores previos involucrados en la producción de bioetanol, aspectos a considerar. Revista Internacional de Contaminación Ambiental, 30, 213-227.

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