4. Conclusion

The traditional fermented rice beer plays an integral role in the ethnic community throughout the world since the beginning of mankind. It is believed that, in the beginning time beer was consumed as medicine but with the progress of time. It has become a product of pleasure and enjoyment. It has a great medicinal and therapeutic value [12] . The selection of yeast species is the most vital for production and improving good quality of beer with low PAD activity [47] . The study reveals that the rice beer (gora) produced in Tripura has a significant nutritional value and diversity in terms of quality. In the present study, we isolated, identified and characterized an indigenous yeast strain Pichia kudriavzevii NN/SG II from the rice beer “Gora”. The comparative study with industrial strain revealed that the isolated strain can produce higher quality beer in respect of taste, nutritional requirements than industrial strain as per the result depicted in (Table 4, Table 5). This strain can be easily grown in normal temperature with minimum growth requirements. The Koloi tribes of Tripura are producing this indigenous rice beer since long time under household condition with lack of in-depth scientific input and quality standard, a reorientation with scientific input for better results towards manufacturing and increasing shelf-life is very much essential. However, most of the countries of the world have their own traditional staple drink produced in commercial level also. In terms of that this may be a good option to produce our own traditional drink, because there is an immense scope to validate the beneficial properties of this type of traditional beverages through simple scientific techniques with regulations for the sustainable livelihood of the rural people.

Acknowledgements

We are very much thankful to-Biotech Hubof Mizoram University, Mizoram, India for providing their facility.

Nucleotide Sequence Accession Number

The sequences of the ITS rDNA region and the isolated yeast strain reported in this study have been deposited in the GenBank nucleotide sequence databases under Accession No. MG388336.

Conflicts of Interest

The authors have not declared any conflict of interests.

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