Antimicrobial Activity of Terminalia catappa L. Leaf Extracts against Some Clinically Important Pathogenic Microbial Strains
Sumitra Chanda, Kalpna Rakholiya, Rathish Nair
DOI: 10.4236/cm.2011.24027   PDF   HTML     7,534 Downloads   18,033 Views   Citations


Objective: The present study was undertaken to evaluate in-vitro antimicrobial activity of methanol, acetone and N, N-dimethylformamide extracts from leaf of Terminalia catappa L. (Combretaceae). Methods: In vitro antimicrobial activity of all the extracts was done by agar disc diffusion assay. 91 clinically important strains were used for the study, which were both clinical isolates as well as identified strains. Piperacillin and gentamicin were used as standards for antibacterial assay, while nystatin and flucanazole were used as standards for antifungal assay. Antimicrobial activity was determined by measurement of inhibition zone around each paper disc. For each extract three replicate trials were conducted against each organism. Results: The antibacterial activity was more pronounced against bacteria than fungal strains. The Gram positive bacteria were more susceptible than Gram negative bacteria. The methanol extract showed best antibacterial activity. T. catappa leaf extracts showed better antibacterial activity than commercially used antibiotics. Conclusion: Demonstration of antimicrobial activity of T. catappa provides the scientific basis for the use of this plant in the traditional treatment of diseases and may help to discover new chemical classes of antibiotic substances that could serve as selective agents for infectious disease chemotherapy and control. This investigation has opened up the possibility of the use of this plant in drug development for human consumption possibly for the treatment of various infections caused by microbes.

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S. Chanda, K. Rakholiya and R. Nair, "Antimicrobial Activity of Terminalia catappa L. Leaf Extracts against Some Clinically Important Pathogenic Microbial Strains," Chinese Medicine, Vol. 2 No. 4, 2011, pp. 171-177. doi: 10.4236/cm.2011.24027.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] G. M. Cragg and D. J. Newman, “Drugs from Nature: Past Achievements, Future Prospects,” In: M. M. Iwu and J. C. Wootton, Eds., Ethnomedicine and Drug Discovery, Elsevier Science, Amsterdam, 2002, pp. 23-37.
[2] A. A. Allero and A. J. Afolayan, “Antimicrobial Activity of Solanum tomentosum,” African Journal of Biotechnology, Vol. 5, 2006, pp. 369-372.
[3] M. R. F. De Lima, J. De Souza Luna, A. F. Dos Santos, M. C. C. De Andrade, A. E. G. Sant Ana, J. P. Genet, B. Marquez, L. Neuville and N. Moreau, “Antibacterial Activity of Some Brazilian Medicinal Plants,” Journal of Ethnopharmacology, Vol. 105, No. 1-2, 2005, pp. 137-147. doi:10.1016/j.jep.2005.10.026
[4] J. Parekh, N. Karathia and S. Chanda, “Screening of Some Traditionally Used Medicinal Plants for Potential Antibacterial Activity,” Indian Journal of Pharmaceutical Science, Vol. 68, 2006, pp. 832-834. doi:10.4103/0250-474X.31031
[5] J. Parekh and S. Chanda, “Antibacterial and Phytochemical Studies on Twelve Species of Indian Medicinal Plants,” African Journal of Biomedical Research, Vol. 10, 2007, pp. 175-181.
[6] M. H. S. Hediat and N. Marraiki, “Antimicrobial Activity and Phytochemical Analyses of Polygonum aviculare L. (Polygonaceae), Naturally Growing in Egypt,” Saudi Journal of Biological Sciences, Vol. 17, No. 1, 2010, pp. 57-63. doi:10.1016/j.sjbs.2009.12.009
[7] S. Dash, L. K. Nath, S. Bhise and B. Nihar, “Antioxidant and Antimicrobial Activities of Heracleum nepalense D. Don Root,” Tropical Journal of Pharmaceutical Research, Vol. 4, 2005, pp. 341-347.
[8] R. Nair and S. Chanda, “Antibacterial Activity of Some Medicinal Plants against Some Medically Important Bacterial Strains,” Indian Journal of Pharmacology, Vol. 38, 2006, pp. 142-144. doi:10.4103/0253-7613.24625
[9] M. B. Tadhani and R. Subhash, “In vitro Antimicrobial Activity of Stevia rebaudiana Bertoni Leaves,” Tropical Journal of Pharmaceutical Research, Vol. 5, 2006, pp. 557-560.
[10] S. Mandal, M. D. Mandal and N. Pal, “Antibacterial Potential of Azadirechta indica Seed and Bacopa monniera Leaf Extracts against Multidrug Resistant Salmonella enterica Serovar Typhi Isolates,” Archives of Medical Science, Vol. 3, 2007, pp. 14-18.
[11] R. Nair and S. Chanda, “In-vitro Antimicrobial Activity of Psidium guajava L. Leaf Extracts against Clinically Important Pathogenic Microbial Strains,” Brazilian Journal of Microbiology, Vol. 38, 2007, pp. 452-458. doi:10.1590/S1517-83822007000300013
[12] S. Chanda, Y. Baravalia, M. Kaneria and K. Rakholiya, “Fruit and Vegetable Peels―Strong Natural Source of Antimicrobics,” In: A. Mendez-Vilas, Ed., Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, Formatex Research Center, Spain, Vol. 2, 2010, pp. 444-450.
[13] S. Chanda, S. Dudhtra and M. Kaneria, “Antioxidative and Antimicrobial Effects of Seeds and Fruit Rind of Nutraceutical Plants Belonging to the Fabaceae Family,” Food and Function, Vol. 1, 2010, pp. 308-315. doi:10.1039/c0fo00028k
[14] N. Y. Chiu and K. H. Chang, “The Illustrated Medicinal Plants of Taiwan,” SMC Publishing, Inc., Taipei, Vol. 1, 1986, p. 129.
[15] C. C. Lin, Y. L. Chen, J. M. Lin and T. Ujiie, “Evaluation of the Antioxidant and Hepatoprotective Activity of Terminalia catappa,” American Journal of Chinese Medicine, Vol. 25, 1997, pp. 153-161. doi:10.1142/S0192415X97000172
[16] A. N. Nagappa, P. A. Thakurdesai, N. V. Rao and J. Singh, “Antidiabetic Activity of Terminalia catappa Linn. Fruits,” Journal of Ethnopharmacology, Vol. 88, No. 1, 2003, pp. 45-50. doi:10.1016/S0378-8741(03)00208-3
[17] Y. M. Fan, L. Z. Xu, J. Gao, Y. Wang, X. H. Tang, X. N. Zhao and Z. X. Zhang, “Phytochemical and Anti-Inflammatory Studies on Terminalia catappa,” Fitoterapia, Vol. 75, No. 3-4, 2004, pp. 253-260. doi:10.1016/j.fitote.2003.11.007
[18] Y. F. Zhai, J. Yao, Y. M. Fan, L. Z. Xu, J. Gao and X. N. Zhao, “Inhibitory Effects of LR-98 on Proliferation of Hepatocarcinoma Cells,” Journal of Nanjing University of Natural Science, Vol. 37, 2001, pp. 213-217.
[19] L. Z. Xu, J. Gao, L. Zhu, M. Xu, S. Y. Lu, X. N. Zhao and Z. X. Zhang, “Protective Effects of LR-98 on Hepatotoxicity Induced by Carbon Tetrachloride and D-Galactosamine in Mice,” Journal of Nanjing University of Natural Science, Vol. 36, 2000, pp. 197-201.
[20] A. W. Bauer, W. M. M. Kirby, J. C. Sherries and M. Truck, “Antibiotic Susceptibility Testing by a Standardized Single Disk Method,” American Journal of Clinical Pathology, Vol. 45, 1966, pp. 426-493.
[21] J. Parekh and S. Chanda, “In vitro Antimicrobial Activity of Trapa natans L. Fruit Rind Extracted in Different Solvents,” African Journal of Biotechnology, Vol. 6, 2007b, pp. 766-770.
[22] M. J. Pelczar, E. C. S. Chan and N. R. Krieg, “Microbiology Concepts and Applications,” Mc Graw-Hill Inc, New York, 1993.
[23] M. J. Martinez, J. Betancourt, N. Alanso-Gonzalea and A. Jauregui, “Screening of Some Cuban Medicinal Plants for Antimicrobial Activity,” Journal of Ethnopharmacology, Vol. 52, No. 3, 1996, pp. 171-174. doi:10.1016/0378-8741(96)01405-5
[24] R. F. Service, “Antibiotics that Resist Resistance,” Science, Vol. 270, 1995, pp. 724-727. doi:10.1126/science.270.5237.724
[25] S. Kilani-Jaziri, W. Bhouri, I. Skandrani, I. Limem, L. Chekir-Ghedira and K. Ghedira, “Phytochemical, Anti-microbial, Antioxidant and Antigenotoxic Potentials of Cyperus rotundus Extracts,” South African Journal of Botany, Vol. 77, No. 3, 2011, pp. 767-776. doi:10.1016/j.sajb.2011.03.015
[26] D. Penduka, O. O. Okoh and A. I. Okoh, “In-vitro Antagonistic Characteristics of Crude Aqueous and Methanolic Extracts of Garcinia kola (Heckel) Seeds against Some Vibrio Bacteria,” Molecules, Vol. 16, 2011, pp. 2754- 2765. doi:10.3390/molecules16042754
[27] M. K. Z. Yuen, R. W. K. Wong, U. Hagg and L. Sama-ranayake, “Antimicrobial Activity of Traditional Chinese Medicines on Common Oral Bacteria,” Chinese Medicine, Vol. 2, 2011, pp. 37-42. doi:10.4236/cm.2011.22007
[28] L. Stuart, “Infectious Diseases and Rise of Antibiotic Resistance,” Pharmaceutical News, Vol. 3, 1996, p. 21.
[29] H. Babayi, I. Kolo, J. I. Okojun and U. J. J. Ijah, “The Antimicrobial Activities of Methanolic Extracts of Eucalyptus camaldulensis and Terminalia catappa against Some Pathogenic Microorganisms,” Biokemistri, Vol. 16, 2004, pp. 106-111.
[30] M. Kaneria, Y. Baravalia, Y. Vaghasiya and S. Chanda, “Determination of Antibacterial and Antioxidant Potential of Some Medicinal Plants from Saurashtra Region, India,” Indian Journal of Pharmaceutical Sciences, Vol. 71, 2009, pp. 406-412. doi:10.4103/0250-474X.57289
[31] J. E. Kelmanson, A. K. Jager and J. Van staden, “Zulu Medicinal Plants with Antimicrobial Activity,” Journal of Ethnopharmacology, Vol. 69, No. 3, 2000, pp. 241-246. doi:10.1016/S0378-8741(99)00147-6
[32] J. Parekh and S. Chanda, “Screening of Some India Medi-cinal Plants for Antibacterial Activity,” Indian Journal of Pharmaceutical Sciences, Vol. 68, 2006, pp. 835-838. doi:10.4103/0250-474X.31032
[33] V. P. Kumar, S. C. Neelam, H. Padh and M. Rajni, “Search for Antibacterial and Antifungal Agents from Selected Indian Medicinal Plants,” Journal of Ethnopharmacology, Vol. 107, No. 2, 2006, pp. 182-188. doi:10.1016/j.jep.2006.03.013
[34] D. Djenane, J. Yangüela, L. Montanes, M. Djerbal and P. Roncales, “Antimicrobial Activity of Pistacia lentiscus and Satureja montana Essential Oils against Listeria monocytogenes CECT 935 Using Laboratory Media: Efficacy and Synergistic Potential in Minced Beef,” Food Control, Vol. 22, 2011, pp. 1046-1053. doi:10.1016/j.foodcont.2010.12.015
[35] I. Bhattacharjee, A. Ghosh and G. Chandra, “Antimi-crobial Activity of the Essential Oil of Cestrum diurnum L. Solanales: Solanaceae,” African Journal of Biotechnology, Vol. 4, 2005, pp. 371-374.
[36] F. Scazzocchio, M. F. Cometa, L. Tomassina and M. Palmery, “Antimicrobial Activity of Hydrastis canadensis Extract and It’s Major Isolated Alkaloids,” Planta Medica, Vol. 67, 2001, pp. 561-563. doi:10.1055/s-2001-16493

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