Assessment of Antifungal Activity of Some Himalayan Foliose Lichens Against Plant Pathogenic Fungi
Priti Tiwari, Himanshu Rai, Dalip Kumar Upreti, Suman Trivedi, Preeti Shukla
DOI: 10.4236/ajps.2011.26099   PDF    HTML     6,516 Downloads   13,155 Views   Citations


In vitro antifungal activity of the acetone, methanol and chloroform extracts of four lichen species viz, Bulbothirx setschwanensis, Everniastrum nepalense, Heterodermia diademata, Parmelaria thomsonii were investigated against seven plant pathogenic fungi (Aspergillus flavus, A. fumigatus, Alternaria alternata, Fusarium oxysporum, F. solani, F. roseum and Penicillium citrinum) with reference to commercially available synthetic antifungal drug Ketoconazole (positive control). Lichen secondary metabolites were extracted using Soxhlet extractor and were further recovered through gentle evaporation of solvents in rotatory evaporator. Antifungal activity was analysed employing BauerKirby disc diffusion assay. Acetone and methanol extracts of lichenized fungi were found more effective against tested plant pathogenic fungi. Principal component analysis concluded that though, Ketoconazole was effective against four of the tested plant pathogenic fungi, acetone and methanol extracts of lichens were comparatively more effective against some broad spectrum plant pathogenic fungi (Fusarium oxysporum, F. solani, F. roseum).

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P. Tiwari, H. Rai, D. Upreti, S. Trivedi and P. Shukla, "Assessment of Antifungal Activity of Some Himalayan Foliose Lichens Against Plant Pathogenic Fungi," American Journal of Plant Sciences, Vol. 2 No. 6, 2011, pp. 841-846. doi: 10.4236/ajps.2011.26099.

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The authors declare no conflicts of interest.


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