Construction of Lentivirus-Based Reference Material for RT-PCR Detection of Middle East Respiratory Syndrome Coronavirus and Its Application in External Quality Assessment


Short Retraction Notice
The paper is due to conflicts of interest.
This article has been retracted to straighten the academic record. In making this decision the Editorial Board follows COPE's Retraction Guidelines. Aim is to promote the circulation of scientific research by offering an ideal research publication platform with due consideration of internationally accepted standards on publication ethics. The Editorial Board would like to extend its sincere apologies for any inconvenience this retraction may have caused.
The full retraction notice in PDF is preceding the original paper which is marked "RETRACTED".



Share and Cite:

Zhou, D. and Luo, J. (2018) Construction of Lentivirus-Based Reference Material for RT-PCR Detection of Middle East Respiratory Syndrome Coronavirus and Its Application in External Quality Assessment. Advances in Microbiology, 8, 506-518. doi: 10.4236/aim.2018.87035.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Shirato, K., Yano, T., Senba, S., Akachi, S., Kobayashi, T., Nishinaka, T., et al. (2014) Detection of Middle East Respiratory Syndrome Coronavirus Using Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP). Virology Journal, 11, 139.
[2] Mackay, I.M. and Arden, K.E. (2015) MERS Coronavirus: Diagnostics, Epidemiology and Transmission. Virology Journal, 12, 222.
[3] Corman, V.M., Eckerle, I., Bleicker, T., Zaki, A., Landt, O., Eschbach-Bludau, M., et al. (2012) Detection of a Novel Human Coronavirus by Real-Time Reverse-Transcription Polymerase Chain Reaction. Eurosurveillance, 17, 3-8.
[4] Pereyaslov, D., Rosin, P., Palm, D., Zeller, H., Gross, D., Brown, C.S., et al. (2014) Laboratory Capability and Surveillance Testing for Middle East Respiratory Syndrome Coronavirus Infection in the WHO European Region, June 2013. Eurosurveillance, 19, 26-34.
[5] Corman, V.M., Muller, M.A., Costabel, U., Timm, J., Binger, T., Meyer, B., et al. (2012) Assays for Laboratory Confirmation of Novel Human Coronavirus (hCoV-EMC) Infections. Eurosurveillance, 17, 2-10.
[6] Blaise-Boisseau, S., Hennechart-Collette, C., Guillier, L. and Perelle, S. (2010) Duplex Real-Time qRT-PCR for the Detection of Hepatitis A Virus in Water and Raspberries Using the MS2 Bacteriophage as a Process Control. Journal of Virological Methods, 166, 48-53.
[7] Dreier, J., Stormer, M. and Kleesiek, K. (2005) Use of Bacteriophage MS2 as an Internal Control in Viral Reverse Transcription-PCR Assays. Journal of Clinical Microbiology, 43, 4551-4557.
[8] Rolfe, K.J., Parmar, S., Mururi, D., Wreghitt, T.G., Jalal, H., Zhang, H., et al. (2007) An Internally Controlled, One-Step, Real-Time RT-PCR Assay for Norovirus Detection and Genogrouping. Journal of Clinical Virology, 39, 318-321.
[9] Trombley, A.R., Wachter, L., Garrison, J., Buckley-Beason, V.A., Jahrling, J., Hensley, L.E., et al. (2010) Short Report: Comprehensive Panel of Real-Time TaqMan (TM) Polymerase Chain Reaction Assays for Detection and Absolute Quantification of Filoviruses, Arenaviruses, and New World Hantaviruses. American Journal of Tropical Medicine and Hygiene, 82, 954-960.
[10] Towner, J.S., Sealy, T.K., Ksiazek, T.G. and Nichol, S.T. (2007) High-Throughput Molecular Detection of Hemorrhagic Fever Virus Threats with Applications for Outbreak Settings. The Journal of Infectious Diseases, 196, S205-S212.
[11] Reusken, C., Niedrig, M., Pas, S., Anda, P., Baize, S., Charrel, R., et al. (2015) Identification of Essential Outstanding Questions for an Adequate European Laboratory Response to Ebolavirus Zaire West Africa 2014. Journal of Clinical Virology, 62, 124-134.
[12] Wang, G.J., Sun, Y., Zhang, K., Jia, T.T., Hao, M.J., Zhang, D., et al. (2015) External Quality Assessment of Molecular Detection of Ebola Virus in China. PLoS ONE, 10, e0151695.
[13] Shahkarami, M., Yen, C., Glaser, C., Xia, D.X., Watt, J. and Wadford, D.A. (2015) Laboratory Testing for Middle East Respiratory Syndrome Coronavirus, California, USA, 2013-2014. Emerging Infectious Diseases, 21, 1664-1666.
[14] Papanikolaou, E., Kontostathi, G., Drakopoulou, E., Georgomanoli, M., Stamateris, E., Vougas, K., et al. (2013) Characterization and Comparative Performance of Lentiviral Vector Preparations Concentrated by Either One-Step Ultrafiltration or Ultracentrifugation. Virus Research, 175, 1-11.

Copyright © 2022 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.