Anticancer and Antioxidant Activities of Some Algae from Western Libyan Coast

DOI: 10.4236/ns.2018.107025   PDF   HTML   XML   626 Downloads   1,426 Views   Citations


Seaweeds are considered as one of the largest biomass producers in marine environment that is rich in bioactive metabolites and a source of natural ingredients for functional foods. The potential antioxidant activity and the potential inhibition of Caco2 cell proliferation, of crude extracts of: Chlorophyta (Ulva lactuca, and Codium tomentosum), Phaeophyta (Cystoseira crinita, Cystoseira stricta, and Sargassum vulgare), and Rhodophyta (Gelidium latifolium, Hypnea musciformis, and Jania rubens) were collected from western Libyan coast and evaluated in vitro. The antioxidant activity was determined by reducing power and DPPH assays while cell proliferation, morphological changes and the cell cycle arrest were assessed by MTT, inverted light microscope and flow cytometry methods respectively. The polyphenols and flavonoids rich extracts showed remarkable reducing power and antiradical properties. After exposure of Caco2 cells to various concentrations of extracts (50, 100, 150 and 200 μg/mL) especially from brown algae for 72 h, cell proliferation was reduced significantly. The antiproliferative effect of algae extracts was correlated with their polyphenol and flavonoid contents. Cell cycle analysis further showed that cells were arrested in G phases along with an increment in sub-diploidal cell population (sub-G) after extract application. These results imply that seaweeds which are rich in bioactive compounds may be used in anticancer drug research programs. However, further investigations are essential to reveal the molecular mechanisms of the anticancer activities of these algae.

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Alghazeer, R. , Howell, N. , El-Naili, M. and Awayn, N. (2018) Anticancer and Antioxidant Activities of Some Algae from Western Libyan Coast. Natural Science, 10, 232-246. doi: 10.4236/ns.2018.107025.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Fitton, J.H. (2006) Antiviral Properties of Marine Algae. In: Critchley, A.T., Ohno, M. and Largo, D.B., Org., World Seaweed Resources: An Authoritative Reference System, ETI Information Services, UK, DVD-Rom.
[2] Newman, D.J., Cragg, G.M. and Snader, K.M. (2003) Natural Products as Source of New Drugs over the Period 1981-2002. Journal of Natural Products, 66, 1022-1037.
[3] Stirk, W.A., Reinecke, D.L. and Staden, J. (2007) Seasonal Variation in Antifungal, Antibacterial and Acetylcholinesterase Activity in Seven South African Seaweeds. Journal of Applied Phycology, 19, 271-276.
[4] Alghazeer, R., Whida, F., Gammoudi, F., Nailia, M. and Abduelrhman, E. (2013) In Vitro Antibacterial Activity of Alkaloid Extracts from Green, Red and Brown Macroalgae from Western Coast of Libya. African Journal of Biotechnology, 51, 7086-7091.
[5] Lindequist, U. and Schweder, T. (2001) Marine Biotechnology. In: Rehm, H.J. and Reed, G., Eds., Biotechnology, Wiley-VCH, Weinheim, Vol. 10, 441-484.
[6] Rabanal, M., Ponce, N., Navarro, D.A., Gómez, R.M. and Stortz, C.A. (2014) The System of Fucoidans from the Brown Seaweed Dictyota dichotoma: Chemical Analysis and Antiviral Activity. Carbohydrate Polymers, 101, 804-811.
[7] Zandi, K., Ahmadzadeh, S., Tajbakhsh, S., Rastian, Z., Yousefi, F., Farshadpour, F. and Sartavi, K. (2010) Anticancer Activity of Sargassum oligocystum Water Extract against Human Cancer Cell Lines. European Review for Medical and Pharmacological Sciences, 14, 669-673.
[8] Ale, M.T., Maruyama, H., Tamauchi, H., Mikkelsen, J.D. and Meyer, A.S. (2011) Fucoidan from Sargassum sp. and Fucus vesiculosus Reduces Cell Viability of Lung Carcinoma and Melanoma Cells in Vitro and Activates Natural Killer Cells in Mice in Vivo. International Journal of Biological Macromolecules, 49, 331-336.
[9] Zubia, M., Fabre, M.S., Kerjean, V., et al. (2009) Antioxidant and Antitumoural Activities of Some Phaeophyta from Brittany Coasts. Food Chemistry, 116, 693-701.
[10] Lekameera, R., Vijayabaskar, P. and Somasundaram, S.T. (2008) Evaluating Antioxidant Property of Brown Alga Colpomenia sinuosa (DERB. ET SOL). African Journal of Food Science., 2, 126-130.
[11] Cox, S., Abu-Ghannam, N. and Gupta, S. (2010) An Assessment of the Antioxidant and Antimicrobial Activity of Six Species of Edible Irish Seaweeds. International Food Research Journal, 17, 205-220.
[12] Keyrouz, R., Abasq, M.L. and Le Bourvellec, C. (2011) Total Phenolic Con-Tents, Radical Scavenging and Cyclic Voltammetry of Seaweeds from Brittany. Food Chemistry, 126, 831-836.
[13] Ranjala, R., Yanxia, L., Valerie, J., Paul and Hendrik, L. (2013) Cultivated Sea Lettuce Is a Multiorgan Protector from Oxidative and Inflammatory Stress by Enhancing the Endogenous Antioxidant Defense System. Cancer Prevention Research, 6, 989.
[14] Zubia, M., Robledo, D.L. and Freile-Pelegrin, Y. (2007) Antioxidant Activities in Tropical Marine Macroalgae from the Yucatan Peninsula, Mexico. Journal of Applied Phycology, 19, 449-458.
[15] Mittler, R. (2002) Oxidative Stress, Antioxidants and Stress Tolerance. Trends in Plant Science, 7, 405-410.
[16] Yuan, Y.V., Carrington, M.F. and Walsh, N.A. (2005) Extracts from Dulse (Palmaria palmata) Are Effective Antioxidants and Inhibitors of Cell Proliferation in Vitro. Food and Chemical Toxicology, 43, 1073-1081.
[17] Yoshie, Y., Wang, W., Hsieh, Y.P. and Suzuki, T. (2002) Compositional Difference of Phenolic Compounds between Two Seaweeds, Halimeda spp. Journal of the Tokyo University of Fisheries, 88, 21-24.
[18] Xu, W.H., Ding, Y., Jacob, M.R., Agarwal, A.K., Clark, A.M., Ferreira, D., Liang, Z.S. and Li, X.C. (2009) Puupehanol, a Sesquiterpenehydroquinone Derivative from the Marine Sponge Hyrtios sp. Bioorganic & Medicinal Chemistry Letters, 19, 6140-6143.
[19] Thomas, T.R.A., Kaulekar, D.P. and Lokabarathi, P.A. (2010) Marine Drugs from Sponge-Microbe Association: A Review. Marine Drugs, 8, 1417-1468.
[20] Zhao, M., Yang, B., Wang, J., Liu, Y., Yu, L. and Jiang, Y. (2007) Immunomodulatory and Anticancer Activities of Flavonoids Extracted from Litchi (Litchi chinensis Sonn.) Pericarp. International Immunopharmacology, 7, 162-166.
[21] Gawron, A. and Kruk, I. (1992) Cytotoxic Effect of Xanthotoxol (8-hydroxypsoralen) on TCTC Cells in Vitro. Polish Journal of Pharmacology & Pharmacy, 44, 51-57.
[22] Duan, X., Wu, G. and Jiang, Y. (2007) Evaluation of the Antioxidant Properties of Litchi Fruit Phenolics in Relation to Pericarp Browning Prevention. Molecules, 12, 759-771.
[23] Wang, B.G., Zhang, W.W., Duan, X.J. and Li, X.M. (2009) In Vitro Antioxidative Activities of Extract and Semi-Purified Fractions of the Marine Red Alga, Rhodomela confervoides (Rhodomelaceae). Food Chemistry, 113, 1101-1105.
[24] Vasanthi, H.R., Rajamanickam, G.V. and Saraswathy, A. (2004) Tumoricidal Effect of the Red Algae Acanthophora spicifera on Ehrlich’s Ascites Carcinoma in Mice. Seaweed Research and Utilization, 25, 217-224.
[25] Heo, S.J., Park, P.J., Park, E.J., Kim, S.E.K. and Jeon, Y.J. (2005) Antioxidant Activity of Enzymatic Extracts from a Brown Seaweed Ecklonia cava by Electron Spin Resonance Spectrometry and Comet Assay. European Food Research and Technology, 221, 41-47.
[26] Athukorala, Y., Jung, W.K., Vasanthan, T. and Jeon, Y.J. (2006) An Anticoagulative Polysaccharide from an Enzymatic Hydrolysate of Ecklonia cava. Carbohydrate Polymers, 66, 184-191.
[27] Senevirathne, M.S., et al. (2006) Antioxidant Potential of Ecklonia cava on Reactive Oxygen Species Scavenging, Metal Chelating, Reducing Power and Lipid Peroxidation Inhibition. Food Science and Technology International, 12, 27-38.
[28] Marinova, D., Ribarova, F. and Atanassova, M. (2005) Total Phenolics and Total Flavonoids in Bulgarian Fruits and Vegetables. Journal of Chemical Technology & Biotechnology, 40, 255-260.
[29] Park, Y.-S., Jung, S.-T., Kang, S.-G., Heo, B.K., Arancibia-Avila, P., Toledo, F., Drzewiecki, J., Namiesnik, J. and Gorinstein, S. (2008) Antioxidants and Proteins in Ethylene-Treated Kiwifruits. Food Chemistry, 107, 640-648.
[30] Dandlen, S.A., Lima, A.S., Mendes, M.D., Miguel, M.G., Faleiro, M.L., Sousa, M.J., Pedro, L.G., Barroso, J.G. and Figueiredo, A.C. (2010) Antioxidant Activity of Six Portuguese Thyme Species Essential Oils. Flavour and Fragrance Journal, 25, 150-155.
[31] Oyaizu, M. (1986) Studies on Products of Browning Reactions: Antioxidative Activities of Products of Browning Reaction Prepared from Glucosamine. The Japanese Journal of Nutrition and Dietetics, 44, 307-315.
[32] Denkert, C., Furstenberg, A., Daniel, P.T., Koch, I., Kobel, M., Weichert, W., Siegert, A. and Hauptmann, S. (2003) Induction of G0/G1 Cell Cycle Arrest in Ovarian Carcinoma Cells by the Anti-Inflammatory Drug NS-398, But Not by COX-2-Specific RNA Interference. Oncogene, 22, 8653-8661.
[33] Sheih, C., Fang, T., Wu, T. and Lin, P. (2010) Anticancer and Antioxidant Activities of the Peptide Fraction from Algae Protein Waste. Journal of Agricultural and Food Chemistry, 58, 1202-1207.
[34] Sanja, S.D., Sheth, N.R., Patel, N.K., Dhaval, P. and Biraju, P. (2009) Characterization and Evaluation of Antioxidant Activity of Portulaca oleracea. International Journal of Pharmacy and Pharmaceutical Sciences, 1, 74-84.
[35] Indu, H. and Seenivasan, R. (2013) In Vitro Antioxidant Activity of Selected Seaweeds from Southeast Coast of India. International Journal of Pharmacy and Pharmaceutical Sciences, 474-484.
[36] Ksouri, R., Megdiche, W., Falleh, H., Trabelsi, N., Boulaaba, M., Smaoui, A. and Abdelly, C. (2008) Influence of Biological, Environmental and Technical Factors on Phenolic Content and Antioxidant Activities of Tunisian Halophytes. Comptes Rendus Biologies, 331, 865-873.
[37] Moghadamtousi, S., Karimian, H., Khanabdali, R., Razavi, M., Firoozinia, M., Zandi, K. and Abdul Kadir, H. (2014) Anticancer and Antitumor Potential of Fucoidan and Fucoxanthin, Two Main Metabolites Isolated from Brown Algae. The Scientific World Journal, 2014, Article ID: 768323.
[38] Karthick, N., Anees Fathimal, M., Ramesh, K., Sridhar, H., Natrajan, M., Divya, V.V., Umavanitha, M. and Umamaheswari, S. (2014) Screening of Phytochemicals and Antimicrobial Activity of Caulerpa scalpelliformis Collected from Manapad Coast, Tuticorin District, Tamilnadu, South India. Journal of Coastal Life Medicine, 2, 107-111.
[39] Salucci, M.A., Stivala, L., Maiani, G., Bugianesi, R. and Vannini, V. (2002) Flavonoids Uptake and Their Effect on Cell Cycle of Human Colon Adenocarcinoma Cells (CaCo2). British Journal of Cancer, 86, 1645-1651.
[40] Du, G., Lin, H.M., Wang, M., Zhang, S., Wu, X., Lu, L., Ji, L. and Yu, L. (2010) Quercetin Greatly Improved Therapeutic Index of Doxorubicin against 4T1 Breast Cancer by Its Opposing Effects on HIF-1α in Tumor and Normal Cells. Canadian Journal of Physiology and Pharmacology, 65, 277-287.
[41] Plochmann, K., Korte, G., Koutsilieri, E., Richling, P., Rethwilm, A., Schreier, P. and Schelle, C. (2007) Structure-Activity Relationships of Flavonoid-Induced Cytotoxicity on Human Leukemia Cells. Archives of Biochemistry and Biophysics, 460, 1-9.
[42] Kuttan, G., Kumar, K.B., Guruvayoorappan, C. and Kuttan, R. (2007) Antitumor, Anti-Invasion, and Antimetastatic Effects of Curcumin. Advances in Experimental Medicine and Biology, 595, 173-184.
[43] Lamoral-Theys, D., Pottier, L., Dufrasne, F., Neve, J., Dubois, J., Kornienko, A., Kiss, R., Ingrassia, L., Lamoral-Theys, D., Pottier, L., Dufrasne, F., Neve, J., Dubois, J., Kornienko, A., Kiss, R. and Ingrassia, L. (2010) Natural Polyphenols That Display Anticancer Properties through Inhibition of Kinase Activity. Current Medicinal Chemistry, 17, 812-825.
[44] Minko, T., Dharap, S.S. and Fabbricatore, A.T. (2003) Enhancing the Efficacy of Chemotherapeutic Drugs by the Suppression of Antiapoptosis Cellular Defense. Cancer Detection and Prevention, 27, 193-202.
[45] Palozza, P., Torelli, C., Boninsegna, A., et al. (2009) Growth-Inhibitory Effects of the Astaxanthin-Rich Alga Haematococcus pluvialis in Human Colon Cancer Cells. Cancer Letters, 283, 108-117.
[46] Moungjaroen, J., Nimmannit, U., Callery, P.S., Wang, L., Azad, N., Lipipun, V., Chanvorachote, P. and Rojanasakul, Y. (2006) Reactive Oxygen Species Mediate Caspase Activation and Apoptosis Induced by Lipoic Acid in Human Lung Epithelial Cancer Cells through Bcl-2 Downregulation. Journal of Pharmacology and Experimental Therapeutics, 319, 1062-1069.
[47] McCullagh, M. (2008) Natural Product Pharmaceuticals—The Third Generation.

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