Bone marrow increases human islets insulin positive cells in co-culture: quantification with flow cytometry
Zhengke Wang, Fang Xiong, Mary Hassani, John Z. Q. Luo, LuGuang Luo
DOI: 10.4236/jdm.2011.14015   PDF   HTML   XML   3,599 Downloads   6,742 Views   Citations


We have previously demonstrated that allogeneic human bone marrow (BM) supports human islet function and longevity in vitro. We hypothesize that BM supporting human islets may include to increase β-cell in cultured islets. In this study, we developed a method to quantify insulin-producing β cells from cultured islets by using immunofluorescent staining and flow cytometry analysis to explore this possibility. The results show that human islets cocultured with BM for 39 days contained a significantly higher number of insulin-positive β cells (42.3% ± 4.5%) compared to the islet-only cultures (1.15% ± 0.78%), and increased insulin release levels evaluated by ELISA is consistent with increased β cells in same culture condition. Human islet culture with BM significantly increase β-cells while islet only culture lost β-cells in same culture period supports the possibility of BM increasing β-cells in cultured islets.

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Wang, Z. , Xiong, F. , Hassani, M. , Luo, J. and Luo, L. (2011) Bone marrow increases human islets insulin positive cells in co-culture: quantification with flow cytometry. Journal of Diabetes Mellitus, 1, 109-117. doi: 10.4236/jdm.2011.14015.

Conflicts of Interest

The authors declare no conflicts of interest.


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