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Genotype × Environmental Interaction on Seed Cotton Yield and Yield Components

DOI: 10.4236/oalib.1103192    361 Downloads   759 Views  


Cotton yield and fibre quality parameters are dependent on the environment in which the crop is grown. A major challenge in crop genotype recommendations is genotype × environment interaction. The identification of cultivars with high adaptability and stability is one of the best ways to address this challenge. Genotype × Environmental interaction on Gosypium hirsutum was investigated. Ten genotypes were planted in a randomised complete block design with three replications. Collected data were subjected to Analysis of Variance (ANOVA) with genotype and location as factors. Additive Main Effect and Multiplicative Interaction model which combines standard analysis of variance with principal component analysis was used to investigate the genotype main effects, environment main effects and the GE interactions. Significant genotype × environment interactions existed for lint yield, boll weights, staple length, and fuzzy seed grade. There were no significant cultivar × site interactions on total seed cotton yield. Cotton cultivars respond differently to different growing conditions implying that, correct choice of varieties by growers for specific production conditions, must be a must to avoid losses due to genotype × environment interactions.

1. Introduction

Cotton is grown in Zimbabwe in diverse environments by smallholder farmers in marginal soils using few inputs and contributing about 99% of the total production in Zimbabwe [1] . Expression of the genetic potential of newly released varieties is affected by the environmental factors or production conditions. High yielding genotype with quality fibre is of economic importance. Genotype × Environmental interaction (GE) of the cotton genotypes is important. Though cotton is adapted to harsh growing conditions, it is sensitive to environmental variations like any other [2] [3] [4] .

In Zimbabwe, cotton varieties that are commercialised have been generally claimed to be adapted to all cotton growing regions of the country [5] . [6] reported that potential yield in excess of 4000 kg per ha as prescribed by the cotton breeders and agronomists has not really been achieved by the farmers. The national average production currently stands at 800 kg per ha [7] [8] [9] . Although [6] pointed out a failure to use fully production package as the cause of lower cotton yields in communal farms, it is interesting to note the effect of environment on the performance of the existing varieties.

Plant breeders claim a wider adaptability of the varieties they recommend to farmers, whilst [10] showed that individual varieties may have limited adaptation because of variety sensitivity to G × E interaction. [11] defined this G × E interaction as the failure of genotypes to achieve the same relative performance in different environments. GE interactions are considered a hindrance to crop improvement in target regions, causing instability in crop yields which have a negative effect on farmers’ income. In cases of staple crops failure, it causes food insecurity at national and household levels [12] [13] [14] . According to [14] G × E interactions offer opportunities for selection of adapted genotypes whilst poor yielders are not exploited.

Productivity of a particular genotype is maximised when it is grown in the adaptable environment. The yield and quality of crops is environmentally dependant, causing specific genotypes to exhibit different phenotypic characteristics under different growing conditions [15] . GE is of great interest when evaluating the stability of genotypes under different environmental conditions [4] .

[16] noted that giving farmers the correct advice on choice of varieties in terms of local stability and adaptability is inevitable where GE studies are done. In this context, evaluating the current and promising genotypes under two major agro- ecological regions to determine the stable and adaptable ones specifically to the major growing districts of the country is very important. The best genotypes have high mean yield and are well adapted and stable (IPCA-1 score closer to 0), [17] [18] [19] [20] . Even though some GE studies have been reported on a number of crops in the whole world [9] [16] [21] [22] almost none has been reported for cotton production in Zimbabwe.

This study evaluated cotton varieties under two distinct environments, undertaken with the main objective of determining the effect of G × E interaction on adaptability and stability of cotton genotypes on total seed cotton yield and yield components.

2. Materials and Methods

2.1. Study Site

The two sites used in this project were Kadoma and Chitekete, which represent the mega and major environments where cotton is being grown in Zimbabwe (Table 1 and Table 2).

2.2. Experimental Design and Germplasm Used

The experiment was laid down in a Randomised complete block design with ten treatments replicated three times. The treatments were cotton genotypes at ten levels. The genotypes consisted of four promising and six released Gossypium

Table 1. Climatic characteristics and conditions of Chitekete and Kadoma locations.

Table 2. Average rainfall data and temperature at Chitekete and Kadoma in 2011-12 cotton growing season in Zimbabwe.

Table 3. The list of variety codes and sources of germplasm used in this project.

hirsutum varieties. These varieties were sourced from the public managed Cotton Research Institute and with one commercial variety coming from a privately managed Quton Seed Company. The list of the variety codes are shown in (Table 3). The sources and phenotypic characteristics of the germplasm used (Table 3).

2.3. Experimental Procedure

The crops were planted in summer of 2011-12 season on ridges after opening the planting farrows using an ox drawn cattle ridger at Chitekete and Kadoma. Compound L fertiliser (N:P:K:S = 5:18:10:8:{0.25B}) at a rate of 250 kg per hectare was banded in the seed furrows using hands. The crop was dry planted at both sites. The seed was hand placed in plots measuring 12 m × 5 m at a rate of 3 - 5 seeds per station. Planting in Chitekete was done on 29 November 2011 whilst planting at Kadoma Cotton Research Institute was done on 6 December 2011. Compound L fertiliser produced by Windmill Private limited was banded at a rate of 250 kg per hectare to the planting furrows using hands. Ammonium nitrate (34.5% N) manufactured by Zimbabwe Fertiliser Company was applied at a rate of 150 kg per hectare to the crop at the ninth week after crop emergence. The crop was thinned to one plant per station to achieve a desired plant population of about 33,333 plants per hectare. Planting spacing was left at 1 m inter-row by 0.3 m within row. At Chitekete, thinning was done 19 days after crop emergence whilst at Kadoma, thinning was done 23 days after crop emergence. Weeding was done to remove any weeds from the trials when necessary by a hand hoe. Weeding at both sites was done three times for the whole season. The following cotton pests were control using the general recommended cotton pest scouting and control protocol developed at CRI in 1993 by the Cotton Research Institute entomology section [5] . The following pests were controlled: aphids (Aphis gosypii), red boll worms (Diaparposis castanea), Heliothes bollworm (Helicoverpa armigera). A total of five sprays were applied at Kadoma whilst six sprays were applied at Chitekete to control various pests. Pests were kept at below the economic thresholds levels following weekly scouting. No irrigation was applied at both sites. The experiments relied on natural rainfall for crop growth.

2.4. Data Collection and Analysis

The total seed cotton yield was weighed using a digital scale after picking. The total yield per plot was the sum total of split cotton bolls picked as bolls samples, cotton picked at pick one, two three and so on. The total lint yield per plot measured in kg per hectares was estimated using the gin out turn percentages. The lint yield was calculated as the ratio of total seed cotton determined by the gin out turn percentage. Total lint yield per plot = GOT (%) × Total seed cotton harvested per plot (kg/ha) [8] .

The total plant population was determined by counting the total plant harvested in each plot and then calculating the ratio of the plants per plot expressed in hectares.

Each plot had an expected maximum plant population of 200 plants. A total of 20 plants were randomly selected and their height was measured using a 2 m ruler. The mean plant height from the 20 plants was considered as the plant height for each genotype in each plot.

The grade of the fuzzy seed was determined using a protocol developed by CRI and edited in 1998 (Table 4).

Seed weight was determined by weighing a total of 100 kennels of ginned seed. The average weight of the 100 seeds was considered as the weight of a single seed in milligrams.

The average weight of each split cotton boll was determined using the same procedure as in the weight of a single seed. A total of 100 boll were picked at random from each plot. All the bolls were weighed using a balance scale supplied by Nicholas Scale Company with an accuracy of 0.01 g. the weight of each boll was estimated as the average boll weight obtained from the 100 boll samples

Table 4. Grading of fuzzy cotton seeds [5] .

picked from each plot.

Gin out turn percentage was estimated using the 100 bolls from each plot. The percentage of lint from each sample was then calculated using simple proportion to determine the gin out turn percentage. GOT = (total seed cotton sample − total ginned seed weights) × 100%/(total weight of ginned sample).

The total number of bolls per plant was estimated by counting the total numbers of husks left on the cotton plant after all the bolls were harvested.

The earliness indices were determined by calculating the ratio of early harvests (pick 1) to the total yield harvested at the end of the picking season.

Data were analysed using Genstat 8.1 for windows release 16 March 2005 [23] . Where significance difference was noted, treatments means were separated using the Fischer’s (1930) Least Significant Difference at P ≤ 0.05. Selections of superior varieties were done using the AMMI-1 analysis with Gernstat 8.1 for windows [24] . The interaction effect of genotypes was analysed using additive main effect and multiplicative interaction (AMMI-1) model [25] whilst GE interaction was analysed by a principal component analysis [25] . Principal Component Analysis (PCA) was used to explain patterns in the GE interaction. It was also used to estimate adaptability or stability of the genotypes across the sites.

3. Results

3.1. GE Effects on Seed Cotton Yield and Yield Components

There were no significant interactions (P > 0.05) of GE on total seed cotton yield (kg/ha). However, the performance of the individual genotypes and sites was highly significant (P < 0.01). Table 5 shows the mean yields of the ten genotypes grown in the two environments, the environment means and the first PCA. Mean yields ranged from 2649 kg/ha for QM301 to 4095 for 644/98/01.

The AMMI-1 analysis model revealed that differences between the environments accounted for (57.6%) of the treatment sum of squares (SS) (Table 6). The genotypes significantly accounted for 34.5% of the total sums of squares whilst GE interaction accounted for 7.9% of the treatment SS. The first interaction PCA was not significant (P > 0.05), (Table 6). It captured 100% of the total variation in the GE interaction SS and 100% of the interaction degrees of freedom.

Figure 1 is a GE biplot of the genotypes across the two sites. LS9219 had the largest positive interaction with the environment with a first PCA score of 17.69. CRIMS2 and QM301 appeared to have similar interaction with the environment but differ significantly in yield (Table 5) and (Figure 1).

CRIMS1 had the largest negative interaction (−14.17) but with a higher yield of 3277 kg per hectare and these genotypes were considered unstable for both environments. LS9219 were more inclined to Chitekete whilst CRIMS2 was more inclined to Kadoma. The environments were also variable in both main effects and interaction (Figure 1). Kadoma and Chitekete had equal IPCA scores with Kadoma having a negative and Chitekete a positive interaction scores. Kadoma had a mean yield of 3852 kg/ha which was above the grand mean whilst Chitekete had a mean yield of 2804 kg/ha which was below the grand mean of 3328 kg/ha. Results at Kadoma indicated that there were no significant differences on yield. At Chitekete, significant differences were noted with 644/98/01 having the highest yield (Table 5). The test varieties ranked 1, 2, 3 and 7 (644/98/01; SZ95/17; 648/01/04; 917/5/7) at Kadoma whilst the same varieties ranked 1, 2, 5 and 9

Table 5. Mean values and rankings of total seed cotton yield (kg/ha) and first PCA scores for two locations in 2011/2012.

*, ** = Significant at P < 0.05 and P < 0.01 respectively; NS = Not Significant; LSD: Least Significant Difference; Means with different letters are significantly different.

Table 6. AMMI-1 Analysis of variance and decomposition of degrees of freedom for total seed cotton yields of cotton genotypes at two sites in 2011-12.

*, ** = Significant at P < 0.05 and P < 0.01 respectively; NS = Not Significant.

Figure 1. AMMI-1 model for seed cotton yield (kg/ha) showing the means of genotypes and environments against their respective IPCA-1 scores for Kadoma and Chitekete and patterns of stability and adaptation.

Figure 2. Genotype total seed cotton mean yields modelled by joint regression for two environments showing environmental sensitivity of different genotypes in 2011/2012.

respectively at Chitekete on total seed cotton yield.

The GE regression of cotton genotypes and environmental interaction indicated that some genotypes exhibited no interaction in terms of changing mean performance across sites (Figure 2). Crossover interaction was noted between the highest yielding genotypes; 648/01/01 and 644/98/01 with the latter being very stable (Figure 2).

The yield rankings of the genotypes were not stable across the two environments. Though significant differences were at Kadoma than Chitekete, average seed cotton yields were quite high. The higher yield in Kadoma is attributed to higher rainfall that was received by the crop. The average rainfall requirements for cotton production are 650 mm per season. In Chitekete, the rainfall was just below the minimum average requirements for cotton production. Yield rankings were changing over the two sites.

The varieties were more adapted to Kadoma than Chitekete. All genotypes noted were more adaptable to Kadoma. This study reported differences on yield performance amongst genotypes supporting [26] observations. [27] indicated that GE interactions minimize the usefulness of genotypes by confounding their yield performance subjecting breeders to scrutinise in depth the yield levels, in multiplication trials to reliability and heritability of important traits [28] . The yields obtained from this study were in agreement with yields obtained from trials by cotton agronomists [5] . Poor yield performance of BC853 was noted in this study. This variety was reported to have performed dismally during a stability and adaptability study of commercial cotton cultivars in Mozambique.

The yields were also lower than those reported in Turkey by Unay et al., 2004 where average total seed cotton yield ranged between 4.13 - 4.9 tonnes per ha in contrary to [19] who recorded lower average yield of 1982 - 3025 kg/ha. More adaptability of 644/98/01 and 648/01/04 to both sites is attributed to the morphology of the crop. 644/98/01 has an indeterminate growth habit whilst 648/01/04 displayed a semi-determinate growth habit. The most stable cultivar was CRIMS2 and 644/98/01. Though 644/98/01 was more adapted to Kadoma, its stability and yield performance was excellent. The other test cultivar 648/01/04 had a higher score and was more adapted to Kadoma than Chitekete. BC853 was poorly adapted to environments in this study. IPCA scores of BC853 were very far away from the origin showing its poor stability across the two sites. Manuelgot equivalent results on BC853 from the study on stability and adaptability of commercial cotton cultivars in Mozambique. CRIMS2 can be considered an average and stable genotype for total seed cotton yield. It had IPCA scores closest to zero and with a mean yield above the grand mean. QM301 was also a stable genotype but with a very low average yield. The environments were also variable in both main effects and interaction. The AMMI model identified 648/01/4 and CRIMS2 as good performers of positive interaction on total seed cotton yield. SZ95/7 and 644/98/01 were identified as good performers on total seed cotton yield but with negative interaction as revealed by the IPCA scores. SZ9314 which was reported as relatively stable in Mozambique, did not perform above the other genotypes in this study.

Cotton genotypes displayed a null, crossover, divergence, and convergence type of interaction (Figure 2). Some genotypes exhibited no interaction in terms of changing mean performance across sites. 644/98/01, SZ9314 and 917/5/7 displayed an additive type of trend which showed no interaction indicating a null G × E interaction according to Ferreira, 2006. 644/98/01, QM301 and 648/01/4 had a cross over type of environmental interaction which depicted a complex environmental interaction. CRIMS1 and 917/5/7 displayed a divergent type of interaction. CRIMS1 and CRIMS2 showed a convergence type of interaction which [18] , descried as simple interaction behaviour.

CRIMS2 showed close to zero IPCA score on the mean seed cotton yield. This genotype had a positive interaction IPCA score with the environment. 644/98/01 had the highest yield and had an interaction PCA score of −5.63. The first four selections based on mean yield as explained by [26] [29] , indicated that CRIMS2, a commercial cultivar, had the best adaptability scores for it had the smallest PCA score and was closer to the origin as explained by [14] [17] [18] [20] [30] . CRIMS2 was stable across both environments. 644/98/01, a promising variety must be considered for its consistence in yield performance. Though it had a relatively higher score of −5.63, it had high yield performance at both environments. 648/01/04 had a very high positive score of 12.01. The results obtained showed that it lacks a broad adaptation [31] to the environments. The results from this study also revealed difficultness in simultaneously selection for yield and stability or adaptation. The varieties with higher yield were less stable. In situations where environmental conditions differ in different localities, specific adaptation must be considered where varieties are selected specifically for a certain mega environment. Farmers are most interested in variety that produces consistent yields under their growing conditions [30] . Hence, information on G × E interaction and stability is very important for breeders and farmers under a set of environments. In this context, 644/98/01 is better considered a variety for production in areas resembling Chitekete conditions whilst 648/01/4 for areas of similar conditions with Kadoma. On the same hand, CRIMS1 can be considered for production at both environments though it has lower mean yield but it was found to be more stable across the two environments on seed cotton yields. Differences amongst genotypes as reported by [26] , on yield stability and adaptability were also noted in this present study. The stability scores, mean yield and variance improve the quality of selection [20] .

3.2. GE Effects on Stability and Adaptation of Cotton Genotypes for Boll Weight and Number of Bolls per Plant

The results obtained from AMMI-1 showed highly significant interactions (P < 0.01) on the weight of cotton bolls. The IPCA scores and the genotypes were also highly significant (p < 0.01) for both. There were no significant differences (P > 0.05) on the weight of the bolls harvested across the environments. Mean boll weight ranged from 6.69 g for CRIMS2 to 7.19 for QM301 (Table 7).

There were no differences (P > 0.05) on the mean boll weights at Chitekete whilst at Kadoma, the genotypes means were highly significant (P < 0.001). Tests varieties ranked 3, 7, 8 and 9. The IPCA scores revealed that 648/01/04 was more stable and adaptable together with the commercial variety CRIMS1 at both sites. There genotypes changed rankings between the two sites.

Table 7. Means of cotton boll weights, rankings and numbers bolls per plant of ten cotton genotypes and first PCA scores grown in 2 environments in 2011-12.

**Significant (P < 0.01), Means with different letters are significantly different.

There were also no significant interactions (P > 0.05) on the total number of bolls harvested per each plant for each genotype. There was no significant difference (P > 0.05) on the bolls harvested per plant. The largest number of bolls was harvested from CRIMS1 (15.22 bolls per plant) whilst BC853 had the least number of bolls per plant of 11.32.IPCA scores were also not significant. The rankings indicated in (Table 7), shows more stability of genotypes on their rankings. The test varieties ranked 2, 3, 4 and 6 for both sites. 644/98/01 had the IPCA score closest to zero. It was more stable adapted than the other cultivars. The genotypes had the highest mean boll weight at Kadoma and highest number of harvested bolls at Chitekete, (Table 7).

QM301 had the highest mean boll weight at Kadoma and the least at Chitekete. The weight of bolls was not stable across sites. Genotypes that had higher population of bolls per plant had relatively lower boll weights. There were changes in rankings of the genotypes across the two environments. High Mean boll weight values greater than 5.00 g observed contradicted to [32] report who obtained lower boll weight values of below 1.500 g. If computed under yield estimations, the total numbers of bolls harvested are in positive correlation with the yields recorded. Under very good growing conditions and maximum crop management, the average number of bolls per plant ranges between 22 and 30 bolls [5] . This number had not been recorded in this study. The results also differ from the 30 - 36.6 bolls per plant as per [32] observations. Slight dry spells that were experienced in January and February contributed to the lower number of harvested bolls per each plant because of a tremendous fruit shedding that occurred during this time.

The average boll weights of the elite cultivars were bigger than the old world varieties with average boll mass that ranges below 5 g per boll. The varieties were changing rankings across the two environments. Genotype QM301 was more adapted to Kadoma and yet very unstable across the two sites. CRIMS1 was more adapted to Chitekete.

Generally, the boll weight was not a stable character. Combined analysis of variance on number of harvested bolls per plant indicated that this trait was stable amongst genotypes. The IPCA scores were very close to zero. This character was not affected by the different environmental conditions. The test genotypes though not more adapted maintained their relative rankings across the two sites and is considered more stable on boll weights and number of harvested bolls per plant.

Higher boll weights and boll numbers are considered the basics for higher yielding. Genotypes with improved boll weights and numbers are candidates for higher yields. CRIMS2 showed good and average stability levels. 644/98/01 was more stable in terms of boll numbers whilst 648/01/4 was more stable on the boll weight.

If seed weight is low, the lint output will be higher for each cotton boll. Varieties with seed weights that are lower have the ability to produce more lint yield as higher percentage of the boll weight will be attributed to the lint. In cases where the seed has more weight, less lint is harvested from the boll. It is important that more dry matter is channelled to the much needed lint than the seed. The seed weights of the varieties were very stable across the two sites. Most genotypes maintained their rankings for each sites. The test varieties were the most stable cultivars on seed weights. 644/98/01 and 648/01/04 had the seeds weights comparable to the least weight. These cultivars indicated to be more suitable for future use on this aspect as they are likely to have more lint yields than seed. [19] indicated that genotypes with large PCA scores have large interaction whilst those with small PCA scores and are closer to zero are considered more stable genotypes. SZ9314 and QM301 had the heaviest seeds and are not well suitable for use in lint production as they tend to partition more of the dry matter to seed than the lint during fibre and seed maturation. Adaptation of the genotypes on the seed weight was maintained across the sites. The test cultivars 917/5/7, 644/98/01 and 648/01/4 displayed the lowest rankings on seed weights and hence they depicted the best seed weight expectation of cotton bolls. CRMS1 showed better stability on seed weights across the two sites. CRIMS1 showed a small and negative interaction. Considering the first PCA scores [19] , 917/5/7 had the largest positive interaction with the environment with a score of 0.3 for staple length. CRIMS1 was more stable in terms of seed weights with QM301 and 648/014 having a similar stability pattern.

3.3. GE Effects on Stability and Adaptation of Cotton Genotypes for Seed Weight

There were no significant interactions (P > 0.05) of genotype × environment on the weights of seed of cotton genotypes planted at two sites. However, significant differences (P < 0.05) were noted on genotypes main effects only. Table 8 shows the results of the effect of genotype × environment on the performance of cotton genotypes on seed weight at Chitekete and Kadoma. The IPCA scores were all below 0.50 and were also not significant (P > 0.05). Mean seed weights ranged from 11.60 mg (917/5/7) to 13.12 mg (QM301) per seed. The mean seed weight was 12.61 mg. QM301 ranked 1 on both sites. The test variety SZ95/7 ranked 4 on both sites in terms of seed weight whilst the other test varieties ranked 9:9; 8:7 and 10:10 for seed index (Table 8).

3.4. GE Effects on Stability and Adaptation of Genotypes for Plant Height

There were no significant GE interactions (P > 0.05) on the performance of cotton genotypes on plant height. There were also no significant differences (P > 0.05) on the environment and genotype main effects on the final plant height of cotton genotypes. Chitekete had taller plants of mean 128.1 cm whilst Kadoma had mean average plant heights of 123.6 cm (Table 9).

The genotypes changed ranks across the environments. QM301 with ranks 2 and 3 for both sites had the smallest IPCA score and was more stable at the two sites.

Table 8. Mean seed weight (mg) of ten cotton genotypes grown in 2 environments means and first PCA scores in 2011/2012.

*Significant (P < 0.05) Means with different letters are significantly different.

Table 9. Mean plant height (cm) of ten cotton genotypes grown in 2 environments and first PCA scores in 2011/2012.

nsnon-significant (P > 0.05) Means with different letters are significantly different.

The results obtained in this study revealed no significant differences amongst genotypes at all sites and on the combined analysis of variance. Changes in rankings of the genotypes over the two sites were not very high. 644/98/01 maintained its relative rank across the two sites. QM301 had a PCA score of 0.03062 and was more stable on plant height. All other genotypes showed IPCA values far away from zero depicting instability. Amongst the test cultivars, SZ95/7 was more adapted to Chitekete with the other test genotypes ranked least in terms of adaptation and stability. The study by [32] indicated an average plant height of 143.3 cm to 180.00 cm, values that are higher than those obtained in this study of 116.7 - 134.4 cm.

3.5. GE Effects on Stability and Adaptation of Genotypes for Crop Earliness Index

The firsts PCA scores and the interactions were not significant, (P > 0.05). The environments had highly significant differences (P < 0.01) between them. Chitekete had the highest maturity index of 78.59% whilst Kadoma had the lowest maturity index of 69.15% (Table 10).

More cotton yield was harvested at Chitekete than Kadoma in the first picking of mature seed cotton. Individual site analysis of variance showed some significant differences (P < 0.05) at Chitekete. Test genotypes ranked 1, 2, 3, and 8 for varieties 644/98/01, 917/5/7, 648/01/04, and SZ95/7 respectively on maturity index. Combined analysis indicated that there were no significant differences

Table 10. Maturity index means (%) and rankings of ten cotton genotypes grown in 2 environments and first PCA scores 2011/2012.

*Significant (P < 0.05) Means with different letters are significantly different.

(P > 0.05) on the genotypes. LS9219 showed the earliest maturity index of 78.41% whilst CRIMS2 had the lowest maturity index of 66.41% (Table 10). 917/5/7 had the least score and was found to be generally more stable across the two sites.

More cotton yield was harvested at Chitekete than Kadoma in the first picking of mature seed cotton. LS9219 showed the earliest maturity index of 78.41% whilst CRIMS2 had the lowest maturity index if 66.41%. Maturity index values from this study are closely related to those by [32] who reported values of 71.0 to 83.0 percent. The temperature ranges of Chitekete as shown in Table 3, indicated that there was more heat units for the crops per day in Chitekete than in Kadoma. Chitekete, though more drier had higher average daily temperatures than Kadoma. This depicted a more closely higher degree days per day as indicated. Kadoma had lower maximum temperatures as compared to Chitekete. Test cultivars 644/98/01 and 648/01/04 ranked 1, and 3 at Chitekete yet 10 and 6 at Kadoma. The results indicated poor stability of these two varieties across the two sites. Adaptation of the test varieties was more inclined to Chitekete than Kadoma. CRIMS2 was more stable for this trait over the two sites. In terms of ranks, it ranked 9 for both sites and its maturity values were rated second on the mean separation.

3.6. GE Effects on Stability and Adaptation of Genotypes for Vegetative Branch Development

The mean number of monopodia per each plant showed no significant interactions (P > 0.05) between the environment and the genotype. The environment had no influence on the number of monopodia branches developed (P > 0.05). For the genotypes, the mean differences of the number of vegetative branches developing were highly significant (P < 0.01). Table 11 show the mean monopodia numbers developed on each plant and the first IPCA scores from AMMI-1 of the cotton genotypes planted in two different environments. The largest population of vegetative branches developed on SZ9314 (3.1 branches) and the least was found on 644/98/01 with a mean of 2.6 branches per plant. The test varieties ranked poorest at both sites, i.e. 6, 7, 8 and 10 rankings for both sites (Table 11).

The largest population of vegetative branches developed on SZ9314 (3.13 branches) and the least was found on 644/98/01 with a mean of 2.6 branches per plant. SZ9314 is a determinate variety which grows very big branches. [32] found the mean monopodia branches to range between 3.13 and 4.13 as well. The genotype with highest mean seed cotton yield, 644/98/01 (2.6 branches) and 648/01/4 (2.833 branches) did not show higher records of vegetative branches. The total numbers of sympodial for the highest yielding genotypes across the sites were significantly lower than that of SZ9314 (3.133 braches). The genotypes produced between two and four monopodia branches agreeing very well with literature on the number of branches developing on G. hirsutum varieties [8] . The number of the vegetative branches affects the reproduction potential of cotton cultivars. Cotton cultivars with less vegetative braches and more of fruiting symbodial

Table 11. Number and rankings of monopodia branches developed on cotton genotypes grown in 2 environments means and first PCA scores in 2011/2012.

**, NS, significant at 0.01%, non-significant respectively. Means with different letters are significantly different.

branches are the most ideal for a cultivated variety. More photosythates were channelled to vegetative growth at the expense of reproduction. It is most ideal to have fewer non fruiting monopodia vegetative branches on cotton. These results are in agreement with [33] about GE interactions.

3.7. GE Effects on Stability and Adaptation of Genotypes for Number of Locules per Mature Cotton Boll

The number of locules per each boll was never influenced by environment or genotype, (P > 0.05), (Table 12). The genotype and environment main effects were also not significant (P > 0.05). Locule number per boll ranged from 4.7 to 5.0. There were also no significant differences (P > 0.05) at individual sites on the number of locules per each boll. Stability and adaptation IPCA scores were relatively lower indicating it as stable character on cotton genotypes. The number of locules per boll on each plant is normally affected by height at which the boll develops. Bolls developing earlier in the season normally have five locules whilst those developing late in the season have four to three locules and are normally found on the upper parts of the stem. The results were contradicting those obtained by [32] who noted an average locule number of 3 - 4 per boll. 917/5/7 maintained rankings over the sites and was more adaptable and stable. The genotypes showed relative stability over the two sites. Test genotypes compared the same with commercial cultivars. Number of locules does not directly influence yield, it indicates the time of development of the harvested bolls. They also indicate

Table 12. Number and rankings of locules developed on cotton genotypes grown in 2 environments means and first PCA scores from2011/2012.

NS: non-significant

the sizes of the bolls. Indeterminate varieties have more bolls with fewer locules than determinate varieties as the later only develops fruits and stops producing more flowers. The results obtained showed that all the varieties used in this study had a mean locule number close to five or five.

3.8. GE Effects on Stability and Adaptation of Genotypes for Number of Fruiting Branches per Plant

There were no significant GE interactions (P > 0.05) on the number of sympodial branches produced by the genotypes. The principal components were also not significant (P > 0.05). There were also no significant differences (P > 0.05) brought by different environments on the number of sympodial developing on each plant. Combined analysis on genotypes, however, showed significant differences (P < 0.05) on the number of sympodial branches produced on them. Analysis of variance at individual sites revealed no significant differences (P > 0.05) amongst genotypes. SZ9314 had the largest mean number of fruiting branches of 7.3 whilst LS9219 had the least mean number of reproductive branches of 5.9 of all the test cultivars, only 644/98/01 had number of branches similar to the best cultivar SZ9314 (Table 13).

SZ9314 had the largest mean number of fruiting branches of 7.3 whilst LS9219 had the least mean number of reproductive branches of 5.9. [32] found that the average number of monopodia per plant ranged between 25 and 30, contradicting with those from this study. The second highest yielding genotype on seed

Table 13. Number and rankings of reproductive branches developed on cotton genotypes grown in 2 environments means and first PCA scores in 2011-12.

*Significant at P<0.05; NS: non-significant. Means with different letters are significantly different.

cotton, 648/01/4 had significantly lower fruiting branches than SZ9314. For the highest yielding variety 644/98/01, the mean number of fruiting branches was comparable to that of SZ9314. The number of reproductive branches developing on each plant was a stable character. IPCA scores were all close to zero. CRIMS2 combined the best was the most stable and adaptable.

4. Conclusions

There was no significant GE interaction on total cotton yield of cotton genotypes grown under the two different environments. There were also no significant GE interactions on seed weight, number of bolls per plant, plant height number of symbodial branches and number of monopodia branches on cotton genotypes. The genotypes showed near to or above average performance across all the environments. Combined analysis revealed that varieties 644/98/01 (4095 kg/ha of seed cotton yield), 648/01/4 (3900 kg/ha), SZ95/7 (3487 kg/ha) and CRIMS2 (3437 kg/ha) were the best varieties on total seed cotton yield across the sites respectively. The best lint yielders were 644/98/01 (1563 k/ha per lint yield), 648/01/4 (1455 kg/a), CRIMS1 (1324 kg/ha) and CRIMS2 (1278 kg/ha). No single genotype exhibited all the best agronomic performance rankings for all the variants tested. The genotype CRIMS2 could be ranked between 1 and 4 for the four most important aspects of total seed cotton yield.

The most adapted genotypes that could be put to future use for Chitekete on seed cotton yield were 648/01/0, 644/98/01, LS9219 and CRIMS2.

Boll weights, plant height, numbers of monopodia developing on cotton plants, number of locules per boll, number of reproductive branches and number of bolls per plant were relatively stable characteristics of cotton genotypes across the two sites. The seed cotton yields, seed weights and maturity index were less stable characteristics across the two environments. On both sites, cultivar CRIMS2 was ranked more stable over a number of characteristics including total seed cotton yield but with lower mean yield of 3437 kg/ha. CRIMS2 can be cultivated in both Chitekete and Kadoma with a realisation of a stable yield performance.

The study also showed that genotype agronomic performance was significantly affected by GE interaction on lint yield, boll weights, and fuzzy seed grade. There were no significant GE interactions on gin outturn percentages on cotton genotypes. The cultivar 648/98/01 ranked best on fibre yield and was a very stable cultivar on this variant.


Current varieties can be grown across Kadoma and Chitekete areas with minimum losses of GE interactions being incurred on total seed cotton yields if the seasons remain relatively the same. Agro-ecological regions 4 and 2a need to be regarded as separate environments when breeding for seed cotton.

The test variety, 644/98/01 must be regarded as a candidate variety for production in cotton producing areas of the country with weather conditions similar to the areas used in this study.

The study of GE interaction in the country need to be further carried out over several sites and seasons to come up with a much defined trend of the GE interaction pattern of cotton genotypes. Though the Additive Main effect and Multiplicative Interaction models used produced yielding results on analysing Genotype × environment interaction, genotype main effects and environment main effects, there is the need to use other statistical methods like regression [34] [35] to further evaluate the stability and adaptability trend of cotton genotypes.


The Cotton Research Institute of Department of Research and Specialist Services for providing resources. The Principal Director of DR & SS.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Mudada, N. , Chitamba, J. , Macheke, T. and Manjeru, P. (2017) Genotype × Environmental Interaction on Seed Cotton Yield and Yield Components. Open Access Library Journal, 4, 1-22. doi: 10.4236/oalib.1103192.


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