Effects of Lignocellulosic in Wood Used as Substrate on the Quality and Yield of Mushrooms
Mercy Badu, Sylvester K. Twumasi, Nathaniel O. Boadi
DOI: 10.4236/fns.2011.27107   PDF    HTML     7,360 Downloads   13,261 Views   Citations


The objective of this study was to find out if the sawdust generated from some of the Ghanaian wood species can be used in the cultivation of pleurotus ostreatus (oyster mushroom) and their subsequent effect on the quality and yield of the mushrooms produced. Sawdust from three Ghanaian wood species (Triplochiton scleraxylon, Ceiba pentandra and Terminalia superba) were collected and their cellulose, hemicelluloses, lignin and nitrogen contents determined using standard methods. Triplochiton scleraxylon gave 46.76%, 15.69%, 27.55%, 0.01% w/w, Ceiba pentandra gave 44.79%, 15.32%, 34.08%, 0.02% w/w and Terminalia superba gave 46.64%, 16.29%, 31.17%, 0.02% w/w of the cellulose, hemicelluloses, lignin and nitrogen content respectively. Compost was then made from each of the wood and used as substrate for the cultivation of pleurotus ostreatus. The highest yield of mushroom was obtained from T. scleraxylon 334g followed by T. superba 277 g and C. pentandra gave the lowest yield of 193 g fresh weight after 3 flushes. The proximate composition of the mushrooms produced gave crude protein ranging 16.33 - 18.20, fat 1.67 - 2.07, carbohydrate 40.86 - 50.53, fibre 4.14 - 6.73 and ash content of 4.40% - 5.80%. The report has shown that the yield and nutritional content of the oyster mushroom on sawdust depends on the chemical constituents such as the cellulose content, the hemicellulose content, the lignin content, the nitrogen content of the particular substrate used. Triplochiton scleraxylon gave the best yield and nutritional content, considering that these substrates are freely available and regarded as “waste”, it can be used to cultivate edible mushrooms to supplement nutritional requirement and source of income to make life better for many people.

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M. Badu, S. Twumasi and N. Boadi, "Effects of Lignocellulosic in Wood Used as Substrate on the Quality and Yield of Mushrooms," Food and Nutrition Sciences, Vol. 2 No. 7, 2011, pp. 780-784. doi: 10.4236/fns.2011.27107.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] D. Cullen and P. J. Kersten, “The Mgcota III, Biochemistry and Molecular Biology,” 2nd Edition, Springer-Verlag Heidelberg, Berlin, 2004, pp. 249-273.
[2] G. Zervakis and A. Philippoussis, “Management of Agro- Industrial Waste through the Cultivation of Edible Mush- Rooms,” Proceedings of the Fourth European Waste Fo-rum Innovation in Waste Management, Millan, 2000, pp. 87-90.
[3] A. Philippousis, G. Zervakis and P. Diamantopoulou, Bioconversion of Lignocellulosic Waste through the Cultivation of the Edible Mustrooms Agrocybe Aegeriha, Volvarialla Volvacea and Pleurotus sp,” World Journal of Microbiology and Biotechnology, Vol. 17, No. 20, 2001, pp. 191-200. doi:10.1023/A:1016685530312
[4] N. Morotroshi, “Chemical Characterisation of Wood and Its Components,” In: D. N.-S. Hon and N. Shiraishi, Eds., Wood Cellulose Chemistry, Marcel Dekker, New York, 1991
[5] T. K. Kirk and T. L. Highly, “Quantitative Changes in Structural Components of Conifer Woods during Decay by White- and Brown-Rot Fungi,” Phytopathology, Vol. 63, 1973, pp. 1338-1342
[6] Z. Bano and S. Rajarathnam, “Pleurotus Mushrooms. Part II, Nutritional Value, Post-Harvest Physiology, Preser- vation and Role as Human Food CRC Critical,” Reviews in Food Science and Nutrition, Vol. 27, No. 2, 1988, pp. 87-158.
[7] P. Roupas, M. Noakes, C. Margetts, J. Keogh and P. Taylor, “Mushrooms and Health National Research Flagships CSIRO,” Global Initiative on Mushrooms and Health Report, June 2010.
[8] Q. A. Mandeel, A. A. Al-Laith and S. A. Mohamed, “Cultivation of Oyster Mushrooms (Pluerotus sp.) on Various Lignocellulosics Wastes,” World Journal of Microbiology and Biotechnology, Vol. 21, No. 4, 2005, pp. 601-607
[9] G. V. Thomas, S. R. Prabhu, M. Z. Reeny and B. M Bopaiah, “Evaluation of Lignocellulosic Biomass from Coconut Palm as Substrate for Cultivation of Pleurotus Sajorcaju (Fr) Singer,” World Journal of Microbiology and Biotechnology, Vol. 14, No. 6, 1998, pp. 879-882. doi:10.1023/A:1008881124903
[10] A. K. Singh, “Cultivation of Oyster Mushroom (Pleurotus sp.) on Sugarcane Residues,” Journal of Mycology and Plant Pathology, Vol. 28, 1998, pp. 240-245.
[11] S. Vetayasuporn, “Bagasse as a Possible Substrate for Pleunotus ostreatus (Fr.) Kummer Cultivation for the Local Mushroom Farms I the Northest of Thailand,” Pakistan Journal of Biology Sciences, Vol. 9, No. 13, 2006, pp. 2512-2515.
[12] S. Vetayasuporn, “The Feasibility of Using Coconut Resi- due as a Substrate for Oyster Mushroom Cultivation,” Biotechnology, Vol. 6, No. 4, 2007, pp. 578-582.
[13] D. Templeton and T. Ehrman, “Determination of Acid- Insoluble Lignin in Biomass,” Laboratory Analytical Procedure No. 003, National Renewable Energy Labora- tory, Golden, CO. 1995.
[14] N. Raghuramulu, M. K. Nair and S. Kalayanasundaram, “A Manual of Laboratory Techniques,” National Institute of Nutrition, ICMR, Hyderabad, India, 1983.
[15] AOAC, “Official Methods of Analysis,” 15th Edition, Association of Official Analytical Chemists, Washington DC, USA, 1990.
[16] M. Dubois, K. A. Giles, J. K. Hamilton, P. A. Rebers and F. Smith, “Calorimetric Methods for Determination of Sugars and Related Substances,” Analytical Chemistry, Vol. 28, No. 3, 1956, pp. 350-356. doi:10.1021/ac60111a017
[17] T. Jefferies, “Biodegradation of Liqnin-Carbohydrate Complex,” Biodegradation, Vol. 1, No. 2-3, 1990, pp. 163-176.
[18] P. Albersheim, “The Primary Cell Wall ‘Plant Biochemis- try’,” Academic Press, New York, 1976, pp. 225-274.
[19] A. J. Clarke, “Biodegradation of Cellulose: Enzymology and Biotechnology,” Technomic, Lancaster, PA, 1997.
[20] B. Keller, “Structural Cell Wall Proteins,” Plant Physiology, Vol. 101, No. 4, 1993, pp. 1127-1130.
[21] S. Vetayasuporn, “Oyster Mushroom Cultivation on Different Cellulose Substrates,” Research Journal of Agriculture and Biological Sciences, Vol. 2, No. 6, 2006, pp. 548-551.
[22] V. A. Aletor, “Compositional Studies On-Edible Tropical Species of Mushrooms,” Food Chemistry, Vol. 54, No. 3, 1995, pp. 265-268. doi:10.1016/0308-8146(95)00044-J
[23] A. Dundar, H. Acay and A. Yildiz, “Yield Performances and Nutritional Contents of Three Oyster Mushroom Species Cultivated on Wheat Stalk,” African Journal of Biotechnology, Vol. 7, No. 19, 2008, pp. 3497-3501.
[24] V. A. Diez and A. Alvarez, “Compositional and Nutritional Studies on Two Wild Mushrooms from Northwest Spain,” Food Chemistry, Vol. 75, No. 4, pp. 417-422. doi:10.1016/S0308-8146(01)00229-1
[25] T. Longvah and Y. G. Deosthale, “Composition and Nutritional Studies on Edible Wild Mushrooms from Northeast India,” Food Chemistry, Vol. 63, No. 3, 1999, pp. 331-334. doi:10.1016/S0308-8146(98)00026-0
[26] A. Yildiz, M. Karakaplan and F. Aydin, “Studies on Pleu- rotus ostreatus (Jacq. Ex Fr.) Kum. Var. salignus (Pers. Ex Fr.) Konr. et Maubl.: Cultivation, Proximate Composition, Organic and Mineral Composition of Carpophores,” Food Chemistry, Vol. 61, No. 1-2, 1998, pp. 127-130. doi:10.1016/S0308-8146(97)00066-6
[27] L. Oi-Wah, “Methods of Chemical Analysis of Mush- Room,” Department of Chemistry, the Chinese University of Hong Kong Shatin, Hong Kong, 1982.
[28] A. Yildiz and M. Karakaplan, “Evaluation of Some Agricultural Wastes for the Cultivation of Edible Mushrooms (Pleurotus ostreatus var salignus),” Journal of Food Science and Technology, Vol. 40, 2003, pp. 290- 292.

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