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Synthesis and Antitumor Activity of Novel Thienopyrimidine Derivatives Containing Thiosemicarbazide Moiety

DOI: 10.4236/oalib.1102876    968 Downloads   1,556 Views   Citations

ABSTRACT

The present study focused on synthesizing a series of novel derivatives of 4-aryl-1-[2-(3-benzyl-4-oxo(3H)-5,6,7,8-tetrahydro[1]benzothieno[2,3-d]pyrimidin-2-ylsulphanyl) acetyl] thiosemicarbazide 5a-d and evaluating their antitumor activity. The structure of the synthesized compounds has been elucidated on the basis of elemental analyses and spectroscopic methods (IR, 1H-NMR, 13C-NMR and MS). The in vitro cytotoxic activity of the synthesized derivatives was evaluated against two human cell lines: prostate cancer (PC-3) and colon cancer (HCT-116).

Subject Areas: Medicinal Chemistry, Organic Chemistry

1. Introduction

In the last few years, cancer attracted great attention and noticeable progress has been made in the discovery and development of modern anticancer drugs. However, despite advances that have led to the development of new therapies, cancer continues to be a major health problem worldwide due to various factors [1] - [3] . Therefore, discovering newer and safer anticancer agents continues to be a challenge [4] .

In the course of finding novel chemical agents that may serve as innovative antitumor agents, quinazoline derivatives are of particular interest [5] . In the last few years, the thieno[2,3-d]pyrimidine core was evaluated as bioisostere of 4-anilinoquinazoline core which included potent marketed anticancer drugs like Gefitinib (IressaTM) [6] , Erlotinib (TarcevaTM) [7] and Tandutinib (MLN518) (phase II clinical trials) [8] . Recently, large number of thieno[2,3-d]pyrimidine derivatives were found to be active against different cancer types exerting their antitumor activities via different mechanisms [9] - [13] . Consequently, the thieno[2,3-d]pyrimidine ring system constitutes an attractive target for the design of new anticancer drugs through wide structure variations.

On the other hand, thiosemicarbazide derivatives exhibit significant biological activities, ranging from antitumor, fungicidal, bactericidal, anti-inflammatory and antiviral activities [14] - [16] ; therefore, the thiosemicarbazide is a highly efficient pharmacophore in drug molecular design. In the present work, novel thieno[2,3-d] py- rimidine derivatives containing thiosemicarbazide moiety 5a-d were synthesized and evaluated for their in vitro antitumor activity against two human cancer cell lines.

2. Results and Discussion

The synthetic strategies adopted for the synthesis of the intermediate and final compounds are illustrated in Scheme 1. The starting compound, ethyl 2-amino-4,5,6,7-tetrahydro [1] benzothiophene-3-carboxylate (1) was prepared according to the well-known Gewald procedure [17] . Reacting 1 with benzyl isothiocyanate in acetonitrile afforded the corresponding 3-benzyl-2-sulfanylthienopyrimidine derivative 2 [18] . Reacting 2 with ethyl

Scheme 1. Synthesis of compounds 3, 4, 5a-d.

chloroacetate in the presence of KOH followed by acidification with dil. HCl yielded the ester 3 according to Devani et al. [18] . Heating 3 with 99% hydrazine hydrate under reflux yielded the hydrazide derivative 4. The structure of compound 4 was confirmed by IR and 1H-NMR spectroscopy. IR spectrum showed broad band in the range of υ 3296 - 3200 cm−1 which indicated the presence of NH and NH2. Peaks around υ 1674 and 1647 cm−1 are due to the two carbonyl moieties. 1H-NMR spectrum showed the presence of two exchangeable protons overlapped with C6 and C7 of cyclohexanyl protons in the range of δ 1.76 - 1.80 ppm indicating the NH2 in addition to one exchangeable proton at δ 9.33 ppm corresponding to the NH. The mass spectrum displayed the molecular ion at m/z = 400 (0.92%) and the base peak at m/z = 91 (100%). The key intermediate 4 was readily converted to the thiosemicarbazide derivatives 5a-d by heating it with the appropriate aryl isothiocyanates in ethanol under reflux. A special feature in the structure of thiosemicarbazides is the presence of thiourea residue (NH-CS-NH), which can be identified by IR spectral data. Compounds 5a-d exhibited strong absorption in the range of υ 1151 - 1195 cm−1 attributable to C = S. Bands around υ 1680 and 1650 cm−1 accounts for the two carbonyl groups of the pyrimidinone ring and the acetyl thiosemicarbazide respectively. 1H-NMR spectra of 5a-d showed a singlet signal at δ 4.1 ppm assignable to -CH2- flanked between the thienopyrimidin-2-ylsul- phanyl group and carbonyl function. The three D2O exchangeable signals for the NH functions were displayed in the range of δ 9.40 - 10.69 ppm. In addition, the presence of OCH3 group in 5d was confirmed by the presence of a singlet signal at δ 3.77 ppm. Besides, two doublets in the range of δ 6.49 - 6.88 ppm and δ 6.98 - 7.21 ppm were assignable to p-disubstituted phenyl ring in compounds 5b-d.

3. Experimental

All Melting points were determined with Stuart SMP10 apparatus and the values given are uncorrected. IR spectra (KBr, cm−1) were determined on Shimadzu IR 8400s spectrophotometer (Faculty of Pharmacy, Cairo University, Egypt). 1H-NMR and 13C-NMR spectra were recorded on Mercury 300-BB 300 MHz (Microanalytical Center, Cairo University, Egypt) and Bruker 400-BB 400 MHz (Microanalytical Unit, Faculty of Pharmacy, Cairo University, Egypt) spectrophotometers using TMS as an internal standard. Chemical shift values are recorded in ppm on δ scale. Mass spectra were recorded on Hewlett Packard 5988 spectrometer (Microanalytical Center, Cairo University, Egypt). Elemental analyses were carried out at the Regional center for Mycology and Biotechnology, Faculty of Pharmacy, Al Azhar University, Egypt; found values were within ±0.35% of the theoretical ones. Progress of the reactions was monitored by TLC (Thin Layer Chromatography) using aluminum sheets precoated with UV fluorescent silica gel (Merck 60F 254) and visualized using UV lamp. The solvent system used was chloroform: benzene: methanol [9:5:2].

The starting compounds, Ethyl 2-amino-4,5,6,7-tetrahydro[1]benzothiophene-3-carboxylate (1) [17] , 3-ben- zyl-2-sulphanyl-5,6,7,8-tetrahydro[1]benzothieno[2,3-d]pyrimidin-4(3H)-one (2) [18] and 2-Ethoxycarbonyl methylthio-3-benzyl-5,6,7,8-tetrahydro[1]benzothieno[2,3-d]pyrimidin-4(3H)-one (3) [18] were prepared according to reported procedures.

2-(3-benzyl-4-oxo(3H)-5,6,7,8-tetrahydro [1]benzothieno[2,3-d]pyrimidin-2-ylsulphanyl) acetohydrazide (4)

The ester 3 (0.82 g, 0.002 mol) was dissolved in absolute ethanol (25 ml) then hydrazine hydrate (99% - 100%) (0.5 g, 0.48 ml, 0.01 mol) was added and the reaction mixture was stirred in a water bath at 70˚C - 80˚C for 1 h and then for additional 25 h at room temperature. The precipitate formed was filtered, dried and recrystallized from benzene/ absolute ethanol (1:2).

Yield: 53%; mp: 138˚C - 140˚C; IR (KBr, cm−1): 3296 - 3200 (NH and NH2 str.), 3062, 3030 (CH aromatic str.), 2924, 2850 (CH aliphatic str.), 1674, 1647 (2 C = O str.); 1H-NMR (DMSO-d6) δ: 1.76 - 1.80 (m, 6H, 2 CH2 at C-6, C-7 & NH2), 2.72 - 2.78 (m, 2H, CH2 at C-5), 2.85 - 2.87 (m, 2H, CH2 at C-8), 3.91 (s, 2H, SCH2), 5.30 (s, 2H, NCH2C6H5), 7.22 - 7.36 (m, 5H, Ar-H), 9.33 (s, 1H, NH, D2O exchangeable); EIMS (% rel. abundance): 400 (M+, 0.92), 91 (C7H7 ˥+, 100); Anal. Calcd for C19H20N4O2S2 (400.52): C, 56.98; H, 5.03; N, 13.99. Found: C, 57.13; H, 5.09; N, 14.17.

General procedure for synthesis of 4-aryl-1-[2-(3-benzyl-4-oxo(3H)-5,6,7,8-tetrahydro[1]benzothieno[2,3-d] pyrimidin-2-ylsulphanyl) acetyl] thiosemicarbazide (5a-d)

To a solution of the hydrazide 4 (0.4g, 0.001 mol) in absolute ethanol (55 ml), the appropriate isothiocyanate (1.2 mmol) was added and the reaction mixture was heated under reflux for 5 - 8 h. The product precipitated while heating was filtered then stirred with absolute ethanol at room temperature for 30 minutes for purification.

4-Phenyll-1-[2-(3-benzyl-4-oxo(3H)-5,6,7,8-tetrahydro[1]benzothieno[2,3-d]pyrimidin-2-ylsulphanyl)acetyl] thiosemicarbazide (5a)

IR (KBr, cm−1): 3325-3226 (NH str.), 3080, 3040 (CH aromatic str.), 2918, 2848 (CH aliphatic str.), 1683, 1656 (2 C = O str.), 1624 (C = N str.), 1153 (C = S str.); 1H-NMR (DMSO-d6) δ: 1.78 - 1.85 (m, 4H, 2 CH2 at C-6, C-7), 2.72 - 2.80 (m, 2H, CH2 at C-5), 2.85 - 2.88 (m, 2H, CH2 at C-8), 4.10 (s, 2H, SCH2), 5.33 (s, 2H, NCH2C6H5), 7.16-7.44 (m, 10H, Ar-H), 9.50, 9.70, 10.34 (each s, 3H, 3NH, D2O exchangeable); EIMS (% rel. abundance): 535 (M+, 35.80), 91 (C7H7˥+, 100).

4-(4-Bromophenyl)-1-[2-(3-benzyl-4-oxo(3H)-5,6,7,8-tetrahydro[1]benzothieno[2,3-d]pyrimidin-2-ylsulphanyl) acetyl] thiosemicarbazide (5b)

IR (KBr, cm−1): 3194-3107 (NH str.), 3062, 3001 (CH aromatic str.), 2935, 2850 (CH aliphatic str.), 1681, 1650 (2 C = O str.), 1583 (C = C aromatic str.), 1193 (C = S str.); 1H-NMR (DMSO-d6) δ: 1.78 - 1.83 (m, 4H, 2 CH2 at C-6, C-7), 2.70 - 2.79 (m, 2H, CH2 at C-5), 2.82 - 2.86 (m, 2H, CH2 at C-8), 4.10 (s, 2H, SCH2), 5.33 (s, 2H, NCH2C6H5), 6.50 (d, 2H, J = 9 Hz, Ar-H), 7.11 (d, 2H, J = 9 Hz, Ar-H), 7.38 - 7.59 (m, 5H, Ar-H), 10.25, 10.60, 10.69 (each s, 3H, 3NH, D2O exchangeable); EIMS (% rel. abundance): 615 (M+2, 1.62), 613 (M+, 1.83), 295 (C17H15N2OS˥+, 100).

4-(4-Chlorophenyl)-1-[2-(3-benzyl-4-oxo(3H)-5,6,7,8-tetrahydro[1]benzothieno[2,3-d]pyrimidin-2-ylsulphanyl) acetyl] thiosemicarbazide (5c)

IR (KBr, cm−1): 3321 - 3107 (NH str.), 3028, 3010 (CH aromatic str.), 2926, 2850 (CH aliphatic str.), 1689, 1654 (2 C = O str.), 1620 (C = N str.), 1195 (C = S str.); 1H-NMR (DMSO-d6) δ: 1.78 - 1.82 (m, 4H, 2 CH2 at C-6, C-7), 2.70-2.78 (m, 2H, CH2 at C-5), 2.85 - 2.88 (m, 2H, CH2 at C-8), 4.11 (s, 2H, SCH2), 5.32 (s, 2H, NCH2C6H5), 6.54 (d, 2H, J = 8.7 Hz, Ar-H), 7.0 (d, 2H, J = 9 Hz, Ar-H), 7.23 - 7.63 (m, 5H, Ar-H), 9.91, 10.25, 10.42 (each s, 3H, 3NH, D2O exchangeable); 13C NMR (DMSO-d6 ppm) δ: 21.66, 22.36, 24.35, 25.12 (4 CH2 of cyclohexane), 38.67 (SCH2), 40.35 (NCH2C6H5), 118.32 (C4a), 124.43 (C5a), 126.45 (benzyl-C4), 127.81 (benzyl-C2,C6), 127.94 (phenyl-C2, C6), 128.27 (benzyl-C3, C5), 128.48 (phenyl-C3, C5), 131.09 (phenyl-C4), 138.17 (phenyl-C1), 138.27 (C8a), 138.62 (benzyl-C1), 139.94 (C9a), 146.79 (C2), 155.57 (C = O pyrimidinone ring), 156.01 (C = O), 181.43 (C = S); EIMS (% rel. abundance): 571 (M+2, 0.8), 569 (M+, 15.65).

4-(4-methoxyphenyl)-1-[2-(3-benzyl-4-oxo(3H)-5,6,7,8-tetrahydro[1]benzothieno[2,3-d]pyrimidin-2-ylsulphanyl) acetyl] thiosemicarbazide (5d) (Table 1)

IR (KBr, cm−1): 3317-3122 (NH str.), 3030, 3010 (CH aromatic str.), 2927, 2833 (CH aliphatic str.), 1687, 1654 (2 C = O str.), 1622 (C = N str.), 1151 (C = S str.); 1H-NMR (DMSO-d6) δ: 1.77 - 1.81 (m, 4H, 2 CH2 at C-6, C-7), 2.69 - 2.79 (m, 2H, CH2 at C-5), 2.85 - 2.87 (m, 2H, CH2 at C-8), 3.77 (s, 3H, OCH3), 4.10 (s, 2H, SCH2), 5.33 (s, 2H, NCH2C6H5), 6.87 (d, 2H, J = 9 Hz, Ar-H), 7.20 (d, 2H, J = 9 Hz, Ar-H), 7.23 - 7.37 (m, 5H, Ar-H), 9.40, 9.60, 10.32 (each s, 3H, 3NH, D2O exchangeable).; EIMS (% rel. abundance): 565 (M+, 3.47).

4. Biological Activity

Anticancer Screening

The cytotoxic activity of the synthesized compounds was evaluated in vitro against human prostate cancer cell

Table 1. Physical and analytical properties of compounds 5a-d.

line (PC-3) and colon cancer cell line (HCT-116) at five different doses (0, 5, 12.5, 25 & 50 µg/ml). The screening was carried out at the Pharmacology Unit, Cancer Biology Department, National Cancer Institute, Cairo University using Sulforhodamine-B (SRB) assay, applying the method of Skehan et al. [19] as follows:

Cells were plated in 96 multi-well plate (104 cells/well) for 24 h before treatment with the tested compound to allow attachment to the wall of the plate. Different concentrations of the compounds (0, 5, 12.5, 25 and 50 μg /ml) were added to the cell monolayer in triplicate and wells were prepared for each individual dose. Monolayer cells were incubated with the compounds for 48 h at 37˚C in atmosphere of 5% CO2. After 48 h, cells were fixed, washed and stained with Sulforhodamine-B stain. Excess stain was washed with acetic acid and the attached stain was recovered with Tris EDTA buffer. Color intensity was measured in an ELISA reader. The relation between surviving fraction and drug concentration is plotted to get the survival curve of each tumor cell line. For each tested compound, IC50 (the concentration required for 50% inhibition of cell viability) was calculated.

The in vitro cytotoxic activity of the synthesized compounds was evaluated against two human cancer cell lines including cells derived from human prostate cancer (PC-3) and human colon cancer (HCT-116). For comparison purposes, the cytotoxicity of doxorubicin, which is one of the most effective anticancer agents [20] , was evaluated under the same conditions. The relationship between the surviving fraction and drug concentration was plotted to obtain the survival curve of prostate cancer cell line (PC-3) and colon carcinoma cell line (HCT-116). The response parameter calculated was the IC50 value, which corresponds to the concentration required for 50% inhibition of cell viability. Values were calculated from dose-response curves done in triplicates for each compound. The IC50 values in μg/ml and µM are listed in (Table 2 and Table 3) and the results are represented graphically in (Figure 1).

From the analysis of the in vitro observed data, it was found, interestingly, that the bromophenyl derivative 5b was the most active against both PC-3 and HCT-116 cell lines, while the unsubstituted phenyl derivative 5a was the least active against both cell lines. It is clear from the results of cytotoxic screening that the substitution on position 4 of the phenyl ring of thiosemicarbazide moiety is essential for the cytotoxic activity against both cell lines where the substituted derivatives 5b-d exhibited higher activity compared to the phenyl analogue 5a. It

Figure 1. Anticancer activity of the synthesized compounds against PC-3 and HCT-116 cell lines compared to Dox.

Table 2. Results of in vitro cytotoxicity of compounds 5a-d against human prostate cancer cell line (PC-3).

Table 3. Results of in vitro cytotoxicity of compounds 5a-d against human colon cancer cell line (HCT-116).

was observed that replacement of the Br in position 4 of phenyl ring with Cl resulted in decrease in activity where compound 5b (substituted with Br) displayed much more potent anticancer activity than 5c (substituted with Cl) against both cell lines. Moreover, compound 5b exhibited 1.5-fold more potent antitumor activity against PC-3 cell line and 3-fold more potent activity against HCT-116 cell line than DOX. In addition, compound 5c showed moderate activity (IC50 38 µM) against both cell lines. It was found that the 4-OCH3 analogue 5d displayed 1.2-fold more potent activity against PC-3 cell line and 1.3-fold more potent activity against HCT-116 cell line in comparison with DOX.

5. Conclusion

A series of thienopyrimidine derivatives linked to thiosemicarbazide moiety was synthesized, characterized and evaluated for their in vitro anticancer activity against two human cancer cell lines (prostate and colon cancer cell lines). Compounds 5b and 5d showed higher cytotoxic activity against both PC-3 and HCT-116 cell lines compared to DOX. The incorporation of Br in position 4 of phenyl ring in thiosemicarbazide moiety was found to significantly enhance the anticancer activity where compound 5b displayed 1.5-fold more potent activity against PC-3 and 3-fold higher activity against HCT-116 compared to the standard drug DOX. In addition, the incorporation of OCH3 group obviously increased the activity against both cell lines than DOX. The obtained results suggest that thieno[2,3-d]pyrimidines containing thiosemicarbazide scaffold might be suitable candidates for further chemical modification in order to obtain more potent and selective anticancer agents.

Acknowledgements

The authors gratefully acknowledge the department of Cancer Biology, National Cancer Institute, Cairo, Egypt, for carrying out the anticancer screening.

NOTES

*Corresponding author.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Salib, S. , Khalil, O. , Kamel, M. and El-Dash, Y. (2016) Synthesis and Antitumor Activity of Novel Thienopyrimidine Derivatives Containing Thiosemicarbazide Moiety. Open Access Library Journal, 3, 1-7. doi: 10.4236/oalib.1102876.

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