The Distribution and Expression of BAMBI in Breast Cancer Cell Lines

DOI: 10.4236/oalib.1102147   PDF   HTML   XML   706 Downloads   1,249 Views   Citations

Abstract

Bmp and activin membrane-bound inhibitor (BAMBI) was initially described as a pseudo receptor antagonizing TGF-β receptor activation, thus impairing signaling. This study tries to find the expression and its significance of BAMBI in breast cancer and explore the relation between BAMBI and clinical and pathological factors of breast cancer. The expression of BAMBI was studied using three cell lines model and Immunofluorescence staining. The impact of BAMBI expression on breast cell was estimated by Western blot and Semi-quantitative RT-PCR. It was demonstrated that BAMBI protein expressed mainly in the membrane and the cytoplasm close to the membrane in breast cancer cell lines, and the relationship between the expression of BAMBI and the proliferation and migration of breast cancer for the first time. The order of BAMBI mRNA expression in the three breast cancer lines is MDA-MB-435s > MDA-MB-231 > MCF-7. Respectively the difference was significant (P < 0.05). It agrees with the proliferation and migration of the three cell lines. The relative amount of BAMBI protein in MDA-MB-435s, MDA-MB-231 and MCF-7 was 0.963 ± 0.061, 0.957 ± 0.048, 0.769 ± 0.103, and the difference was significant (P < 0.05). The abnormally up-regulation of BAMBI is related to breast carcinogenesis.

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Wang, H. and Cui, Z. (2015) The Distribution and Expression of BAMBI in Breast Cancer Cell Lines. Open Access Library Journal, 2, 1-7. doi: 10.4236/oalib.1102147.

Conflicts of Interest

The authors declare no conflicts of interest.

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