A Comparison of the Growth Characteristics of Arthrospira platensis in Deep Ocean Water, Surface Ocean Water, and Artificial Ocean Water

Yen Lee^1*, Ting-Mao Yang², Jenn-Hung Hsu³

¹Department of Life Science, National Taitung University, Taiwan.
²Katec R & D Corporation, Taitung Branch, Taiwan.
³Center for General Education, National Taitung University, Taiwan.

DOI: 10.4236/oalib.1101984                

Abstract

The use of deep ocean water (DOW) for cooling purposes, as drinking water (after desalination), and in algae cultures has increased in recent years in Taiwan. This study evaluates the growth performance of Arthrospira platensis in deep ocean water (DOW), surface ocean water (SOW), and artificial ocean water (AOW) with and without a supplementary medium (BG-11). During a 14-day growth period, A. platensis had the shortest generation time (5.22 ± 0.38 days) and the highest average daily biomass yield of 0.083 ± 0.007 g/L (dry weight) in the DOW medium without supplementary BG-11. A. platensis had significant better growth rates when cultured in AOW with BG-11 than in SOW without BG-11. However, A. platensis growth rates did not differ (p > 0.05) when cultured in AOW and DOW with BG-11. The results revealed that DOW without supplementary BG-11 is a superior medium for culturing A. platensis.

Keywords

Deep Ocean Water, A. platensis, Growth Rate, Surface Ocean Water, Artificial Ocean Water

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Chen, S.H. and Liao, S.H. (2012) Policy on Utilization of Taiwan Deep Ocean Water Resources and Industry-Drive Strategy. http://www.water.org.tw/simply/twic/PDF/A2-1Policy%20on%20Utilization%20of%20Taiwan%20Deep%20Ocean%20Water%20Resources%20and%20Industry-Drive%20Strategy.pdf
[2]	Nakasone, T. and Akeda, S. (2012) The Application of Deep Sea Water in Japan. http://www.lib.noaa.gov/retiredsites/japan/aquaculture/proceedings/report28/Nakasone.pdf
[3]	Takahashi, M.M. (2005) Research on Deep Ocean Water (DOW) and Its Resource Utilization Developed in Japan. Sino-Japanese DOW Symposium, University of Chinese Culture, Taipei, 1-6.
[4]	Gauthier, M.A.P. (2012) Ocean Thermal Energy Conversion and the Utilization of Ocean Water. Encyclopedia of Life Support Systems. http://www.eolss.net/Eolss-SampleALLChapters/aspx
[5]	Bae, M.S. and Lee, S.C. (2010) Effect of Deep Sea Water on the Antioxidant Activity and Catechin Content of Green Tea. Journal of Medicinal Plants Research, 4, 1662-1667.
[6]	Chen, Y., Li, Y.R., Hsieh, P.F. and Lee, C.S. (2007) Strategies of Developing Deep Ocean Water Industry—Cluster and Value Network Views. Portland International Conference on Management of Engineering and Technology, Portland, 5-9 August 2007, 351-357. http://dx.doi.org/10.1109/picmet.2007.4349349
[7]	Ramadan, M.F., Asker, M.M.S. and Ibrahim, Z.K. (2007) Functional Bioactive Compounds and Biological Activities of Spirulina platensis Lipids. Czech Journal of Food Sciences, 26, 211-222.
[8]	Ayehunie, S., Belay, A., Baba, T.W. and Ruprecht, R.M. (1998) Inhibition of HIV-1 Replication by an Aqueous Extract of Spirulina platensis (Arthrospira paltensis). Journal of Acquired Immune Deficiency Syndromes, 18, 7-12. http://dx.doi.org/10.1097/00042560-199805010-00002
[9]	Ayachi, S.A., Abed, E., Dhifi, W. and Marzouk, B. (2007) Chlorophylls, Proteins and Fatty Acids Amounts of Arthrospira platensis Growing under Saline Conditions. Pakistan Journal of Biological Sciences, 10, 2286-2291. http://dx.doi.org/10.3923/pjbs.2007.2286.2291
[10]	Reichert, C.C., Reinehr, C.O. and Costa, A.V. (2006) Semicontinuous Cultivation of the Cyanobacterium Spirulina platensis in a Closed Photobioreactor. Brazilian Journal of Chemical Engineering, 23, 23-28. http://dx.doi.org/10.1590/S0104-66322006000100003
[11]	Lodi, A., Binaghi, L., de Faveri, D., Carvalho, J.C.M., Converti, A. and Del Borghi, M. (2005) Fed-Batch Mixtrophic Cultivation of Arthrospira (Spirulina) platensis (Cyanophycea) with Carbon Source Pulse Feeding. Annals of Microbiology, 55, 181-185.
[12]	Tokuşoglu, Ö. and ünal, M.K. (2003) Biomass Nutrient Profiles of Three Microalgae: Spirulina platensis, Chlorella vulgaris, and Isochrisis galbana. Journal of Food Science, 68, 1144-1148. http://dx.doi.org/10.1111/j.1365-2621.2003.tb09615.x
[13]	Chronakis, I.S. (2001) Gelation of Editable Blue-Green Algae Protein Isolate (Spirulina platensis Strain Pacifica): Thermal Transitions, Rheological Properties, and Molecular Forces Involved. Journal of Agricultural and Food Chemistry, 49, 888-898. http://dx.doi.org/10.1021/jf0005059
[14]	El-Sayed, A.B. and Abdel-Maguid, A.A. (2010) Immobilized-Microalga Scenedesmus sp. for Biological Desalination of Red Sea Water: II. Effect on Macronutrients Removal. Journal of American Science, 6, 637-643.
[15]	Sukenik, A. and Shelef, G. (1984) Algal Autoflocculation—Verification and Proposed Mechanism. Biotechnology and Bioengineering, 26, 142-147. http://dx.doi.org/10.1002/bit.260260206
[16]	Mary-Leema, J.T., Kirubagaran, R., Vinithkumar, N.V., Dheenan, P.S. and Karthikayylu, S. (2010) High Value Pigment Production from Arthrospira (Spirulina) platensis Cultured in Seawater. Bioresource Technology, 101, 9221-9227. http://dx.doi.org/10.1016/j.biortech.2010.06.120
[17]	Rippka, R., Deruelles, J., Waterbury, J., Herdman, M. and Stanier, R. (1979) Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Journal of General Microbiology, 111, 1-61. http://dx.doi.org/10.1099/00221287-111-1-1
[18]	Wang, T.Y., Liu, H.C. and Lee, Y. (2013) Use of Anthropic Acclimated Spirulina platensis (Arthrospira platensis) Bio-Adsorption in the Treatment of Swine Farm Wastewater. International Journal of Agriculture and Biology, 15, 107-112.
[19]	Leone, D.E. (1963) Growth of Chlorella pyrenoidosa in Recycled Medium. Journal of Applied Microbiology, 11, 427-429.
[20]	Work, K., Havens, K., Sharfstein, B. and East, T. (2005) How Important Is Bacterial Carbon to Planktonic Grazers in a Turbid, Subtropical Lake? Journal of Plankton Research, 4, 357-372. http://dx.doi.org/10.1093/plankt/fbi013
[21]	Chen, Y.C. and Lee, M.C. (2012) Double-Power Double-Heterostructure Light-Emitting Diodes in Microalgae, Spirulina platensis and Nannochoropsis oculata, Cultures. Journal of Marine Science and Technology, 20, 233-236
[22]	Ungsethapand, T., Peerapornpisal, Y. and Whangchai, N. (2009) Production of Spirulina platensis Using Dry Chick Manure Supplemented with Urea and Sodium Bicarbonate. Maejo International Journal of Science and Technology, 3, 379-387.
[23]	Stal, L.J., Staal, M. and Villbrandt, M. (1999) Nutrient Control of Cyanobacterial Blooms in the Baltic Sea. Aquatic Microbial Ecology, 18, 165-173. http://dx.doi.org/10.3354/ame018165
[24]	GÖksan, T., Ayşeűl, Z. and İlknur, A.K. (2007) The Growth of Spirulina platensis in Different Culture Systems under Greenhouse Condition. Turkish Journal of Biology, 31, 47-52.
[25]	Roth, V. (2006) Doubling Time Calculator. http://www.doubling-time.com/compute.php
[26]	Warr, S.R.C., Reed, R.H., Chudek, J.A., Foster, R. and Steward, W.D.B. (1985) Osmotic Adjustment in Spirulina platensis. Planta, 163, 424-429. http://dx.doi.org/10.1007/BF00395153
[27]	Wu, B., Tseng, C.K. and Xiang, W. (1993) Large-Scale Cultivation of Spirulina in Seawater Based Culture Medium. Botanica Marina, 36, 99-102. http://dx.doi.org/10.1515/botm.1993.36.2.99
[28]	Pushparaj, B., Pelosi, E., Carlozzi, P. and Torzillo, G. (1995) Yield and Biochemical Composition of a Marine Cyanobacterium (Nodularia sp.) in Outdoor Culture. Aquatic Microbial Ecology, 9, 13-16. http://dx.doi.org/10.3354/ame009013
[29]	Sandnes, J.M., Ringstad, T., Wenner, D., Heyerdahl, P.H., Källqvist, T. and Gislerød, H.R. (2006) Real-Time Monitoring and Automatic Density Control of Large-Scale Microalgal Cultures Using Near Infrared (NR) Optical Density Sensors. Journal of Biotechnology, 122, 209-215. http://dx.doi.org/10.1016/j.jbiotec.2005.08.034